Housekeeping gene stability in Pesudomonas aeruginosa PAO1 under the pressure of commonly used antibiotics in molecular microbiology assays [PDF]
Frontiers in Microbiology, 2023 Pseudomonas aeruginosa is an opportunistic human pathogen notorious for its remarkable capacity of multi-drug resistance, and has become one of the most important model bacteria in clinical bacteriology research.
Lingning Meng +8 more
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What are housekeeping genes? [PDF]
PLoS Computational Biology, 2022 The concept of "housekeeping gene" has been used for four decades but remains loosely defined. Housekeeping genes are commonly described as "essential for cellular existence regardless of their specific function in the tissue or organism", and "stably ...
Chintan J Joshi +4 more
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Un-biased housekeeping gene panel selection for high-validity gene expression analysis [PDF]
Scientific Reports, 2022 Differential gene expression normalised to a single housekeeping (HK) is used to identify disease mechanisms and therapeutic targets. HK gene selection is often arbitrary, potentially introducing systematic error and discordant results.
Ana I. Casas +9 more
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Virology Journal, 2012 Background One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection.
Kuchipudi Suresh V +10 more
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Housekeeping gene validation for RT-qPCR studies on synovial fibroblasts derived from healthy and osteoarthritic patients with focus on mechanical loading. [PDF]
PLoS ONE, 2019 Selection of appropriate housekeeping genes is essential for the validity of data normalization in reverse transcription quantitative PCR (RT-qPCR). Synovial fibroblasts (SF) play a mediating role in the development and progression of osteoarthritis (OA)
Ute Nazet +5 more
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Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp [PDF]
Scientific ReportsFew studies have investigated the subcellular effects of low temperature on gene expression in shrimp and most other crustaceans. Before gene expression analysis is conducted, suitable housekeeping genes (HKGs) must be confirmed to account for ...
Yen-Po Chen +4 more
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BMC Molecular and Cell Biology, 2023 Background Hypoxic culture conditions have been used to study the impact of oxygen deprivation has on gene expression in a number of disease models. However, hypoxia response elements present in the promoter regions of some commonly used housekeeping ...
Huan Ting Ong +2 more
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Evaluation and validation of housekeeping genes as reference for gene expression studies in pigeonpea (Cajanus cajan) under drought stress conditions. [PDF]
PLoS ONE, 2015 Gene expression analysis using quantitative real-time PCR (qRT-PCR) is a very sensitive technique and its sensitivity depends on the stable performance of reference gene(s) used in the study.
Pallavi Sinha +5 more
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A deep insight into the sialome of male and female aedes aegypti mosquitoes [PDF]
, 2016 Only adult female mosquitoes feed on blood, while both genders take sugar meals. Accordingly, several compounds associated with blood feeding (i.e. vasodilators, anti-clotting, anti-platelets) are found only in female glands, while enzymes associated ...
ARCA', Bruno +3 more
core +11 more sources
Horticulturae, 2021 Quinoa is a gluten-free food crop that contains all the essential amino acids and vitamins. The selection of proper housekeeping and tissue-specific genes is the crucial prerequisite for gene expression analysis using the common approach, real-time ...
Bing He +6 more
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