Results 1 to 10 of about 494,714 (299)

A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity

Microorganisms, 2020
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to limit the spread of the global pandemic we are facing.
Sara Petrillo, Giovanna Carrà, Paolo Bottino, Elisa Zanotto, Maria Chiara De Santis, Jean Piero Margaria, Alessandro Giorgio, Giorgia Mandili, Miriam Martini, Rossana Cavallo, Davide Barberio, Fiorella Altruda   +11 more
doaj   +3 more sources

Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence [PDF]

The Plant Pathology Journal, 2018
Strawberry Fusarium wilt disease, caused by Fusarium oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades in
Sung Won Hong, Da-Ran Kim, Ji Su Kim, Gyeongjun Cho, Chang Wook Jeon, Youn-Sig Kwak   +5 more
doaj   +3 more sources

No control genes required: Bayesian analysis of qRT-PCR data.

PLoS ONE, 2013
BackgroundModel-based analysis of data from quantitative reverse-transcription PCR (qRT-PCR) is potentially more powerful and versatile than traditional methods.
Mikhail V Matz, Rachel M Wright, James G Scott   +2 more
doaj   +5 more sources

qRT-PCR with saliva samples [PDF]

BioTechniques, 2012
Kristie Nybo
doaj   +3 more sources

Identification of Suitable Reference Genes for qRT-PCR Normalization in Kiwifruit

Horticulturae, 2022
Reference genes are used for the correction of qRT-PCR data, and it is necessary to investigate the optimum reference gene under certain conditions. The expression levels of seven traditional reference genes ACT1, ACT2, GAPDH, 18S rRNA, UBQ, TUB and CYP ...
Yuanjie Zhou, Hui Xia, Xinling Liu, Zhiyi Lin, Yuqi Guo, Honghong Deng, Jin Wang, Lijin Lin, Qunxian Deng, Xiulan Lv, Kunfu Xu, Dong Liang   +11 more
doaj   +3 more sources

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets

Emerging Microbes and Infections, 2020
Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method.
Xinjin Liu, Jiangpeng Feng, Qiuhan Zhang, Dong Guo, Lu Zhang, Tao Suo, Wenjia Hu, Ming Guo, Xin Wang, Zhixiang Huang, Yong Xiong, Guozhong Chen, Yu Chen, Ke Lan   +13 more
doaj   +3 more sources

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus. [PDF]

Front Microbiol
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus.
Li L, Guo X, Wang S.
europepmc   +2 more sources

Identification and Evaluation of qRT-PCR Reference Genes in Melanaphis sacchari. [PDF]

Insects
Appropriate reference genes must be selected for accurate qRT-PCR data to conduct a thorough gene expression analysis in the sorghum aphid (Melanaphis sacchari, Hemiptera, Aphididae). This approach will establish a foundation for gene expression analysis and determines the efficacy of RNA interference in the sorghum aphid.
Zou K, Wang T, Guan M, Liu Y, Li J, Liu Y, Du J, Wu D.   +7 more
europepmc   +4 more sources

Real-Time qPCR/qRT-PCR Methods: Standard Curves

BioTechniques, 2010
Kristie Nybo
doaj   +2 more sources

Evaluation of droplet digital qRT-PCR (dd qRT-PCR) for quantification of SARS CoV-2 RNA in stool and urine specimens of COVID-19 patients

Frontiers in Medicine, 2023
IntroductionThere have been a few reports of viral load detection in stool and urine samples of patients with coronavirus disease 2019 (COVID-19), and the transmission of the virus through faecal oral route.
Manohar Shinde, Mallika Lavania, Jatin Rawal, Nutan Chavan, Pooja Shinde   +4 more
doaj   +1 more source