Results 1 to 10 of about 1,108,829 (262)

A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity

Microorganisms, 2020
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to limit the spread of the global pandemic we are facing.
Sara Petrillo, Giovanna Carrà, Paolo Bottino, Elisa Zanotto, Maria Chiara De Santis, Jean Piero Margaria, Alessandro Giorgio, Giorgia Mandili, Miriam Martini, Rossana Cavallo, Davide Barberio, Fiorella Altruda   +11 more
doaj   +3 more sources

Identification of Suitable Reference Genes for qRT-PCR Normalization in Kiwifruit

Horticulturae, 2022
Reference genes are used for the correction of qRT-PCR data, and it is necessary to investigate the optimum reference gene under certain conditions. The expression levels of seven traditional reference genes ACT1, ACT2, GAPDH, 18S rRNA, UBQ, TUB and CYP ...
Yuanjie Zhou, Hui Xia, Xinling Liu, Zhiyi Lin, Yuqi Guo, Honghong Deng, Jin Wang, Lijin Lin, Qunxian Deng, Xiulan Lv, Kunfu Xu, Dong Liang   +11 more
doaj   +3 more sources

Identification of stable miRNA references for qRT-PCR normalization in ovine fasciolosis [PDF]

BMC Genomics
Background Fasciola hepatica (F. hepatica) is a widely prevalent trematode responsible for parasitic infections and remains a major target for control interventions worldwide.
Diana M. Barrero-Torres, Guillem Herrera-Torres, Paula V. Huertas-Abril, Nieves Abril, José Pérez, Pablo J. Rufino-Moya, José M. Suárez-Cárdenas, Sara Zaldívar-López, Álvaro Martínez-Moreno, M. Teresa Ruiz-Campillo, Verónica Molina-Hernández   +10 more
doaj   +2 more sources

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus [PDF]

Frontiers in Microbiology
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus.
Lulu Li, Xiaofan Guo, Shouming Wang
doaj   +2 more sources

qRT-PCR primer sequences.

, 2016
qRT-PCR primer sequences.
Claudia Fabbi (3178194), Stefano Forte (93983), Raffaella Giuffrida (3178185), Rosario Gulino (496644), Giovanna Calabrese (496645), Elisa Figallo (3178182), Francesco Fullone (3178191), Cristina Colarossi (129957), Rosario Giuffrida (3178188), Rosalba Parenti (496646), Debora Lo Furno (669799), Lorenzo Memeo (129955)   +11 more
core   +3 more sources

Evaluation of droplet digital qRT-PCR (dd qRT-PCR) for quantification of SARS CoV-2 RNA in stool and urine specimens of COVID-19 patients

Frontiers in Medicine, 2023
IntroductionThere have been a few reports of viral load detection in stool and urine samples of patients with coronavirus disease 2019 (COVID-19), and the transmission of the virus through faecal oral route.
Manohar Shinde, Mallika Lavania, Jatin Rawal, Nutan Chavan, Pooja Shinde   +4 more
doaj   +1 more source

Quantification of Pathogenicity Genes Expression in Pectobacterium carotovorum by qRT-PCR [PDF]

Journal of Agricultural Chemistry and Biotechnology, 2021
Bacterial soft rot disease is one of the most important and widespread bacterial diseases that cause destructive losses to the crop in a matter of days. The disease causes severe many vegetable crops including potato.
K. A. Amein, Zynab Morsy, K. A. Abo Elyousr, A. E. Elfarash   +3 more
doaj   +1 more source

An Improved Duplex Real-Time Quantitative RT-PCR Assay with a Canine Endogenous Internal Positive Control for More Sensitive and Reliable Detection of Canine Parainfluenza Virus 5

Veterinary Sciences, 2023
A duplex real-time quantitative reverse transcription-polymerase chain reaction (dqRT-PCR) assay was successfully developed to simultaneously detect canine parainfluenza virus 5 (CPIV5) and a canine endogenous internal positive control (EIPC) in canine ...
Gyu-Tae Jeon, Hye-Ryung Kim, Yeun-Kyung Shin, Oh-Kyu Kwon, Hae-Eun Kang, Oh-Deog Kwon, Choi-Kyu Park   +6 more
doaj   +1 more source

Clinical Value of S-poly (T) plus Real-time PCR in Detection of Liver Cancer-related miR-199

Zhongliu Fangzhi Yanjiu, 2020
Objective To explore the advantages and disadvantages of S-poly(T) plus qRT-PCR and Poly (A) qRT-PCR methods in detecting peripheral blood serum miR-199.
WU Juan, ZHANG Yaqin, WU Yunbin, CHEN Min, YU Zhou, CAO Yingping   +5 more
doaj   +1 more source

Spit or Swab? The Diagnostic Accuracy of Saliva-Based Testing as a Sars-Cov-2 Surveillance Tool

Philippine Journal of Pathology, 2022
Background. Nasopharyngeal swab/oropharyngeal swab (NPS/OPS) qRT-PCR is the gold standard for detecting SARS-CoV-2. However, it has its own limitations including cost and invasiveness.
Raymundo Lo, Farrah Kristine Santiago, Grig Misiona, Melani Sionzon, Danielle Anne Gonong, Jill Jaime, Jan Roman Ayco, Aquiles Joseph Lira, Erik Francis Rarugal, Jon Carlo Crisostomo, Manuel Bautista Jr., Kenneth Chrysostom Ibarra, Eunice Jane Ciriaco, Arielle Trish Zamora, Kimberly Abundo, Emmylou Tarnate, Jonathan Nario, Jan Joseph Hernandez, Kathleen Mae Montes, Ma. Anabella Recio   +19 more
doaj   +1 more source