Results 41 to 50 of about 1,108,829 (262)

Experimental assessment of robust reference genes for qRT-PCR in lung cancer studies

Frontiers in Oncology, 2023
Stable internal reference genes are crucial for quantitative real-time PCR (qRT-PCR) analyses in lung cancer studies. Widely used reference genes are mostly chosen by intuition or from pan-cancer transcriptome data and lack experimental validation by qRT-
Wei Gu, Wei Gu, Yubin Wang, Ran Xu, Jiamin Li, Jingjie Jin, Jing Zhao, Yang Chen, Yuanzhi Lu, Gong Zhang   +9 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

qRT-PCR primers.

, 2016
qRT-PCR primers.
Marc P. Stemmler (176442), Tim Pieters (3074160), Dagmar Wirth (48093), Vanessa Andries (74590), Danny Huylebroeck (124349), Kelly Lemeire (3074157), Jody J. Haigh (426911), Riet De Rycke (416524), Jolanda van Hengel (253211), Agata Stryjewska (469825), Frans van Roy (3074154), Lieven Haenebalcke (734219), Geert Berx (12850), Steven Goossens (426910), Tino Hochepied (493717)   +14 more
core   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

A quantitative real time PCR method to analyze T cell receptor Vβ subgroup expansion by staphylococcal superantigens

Journal of Translational Medicine, 2010
Background Staphylococcal enterotoxins (SEs), SE-like (SEl) toxins, and toxic shock syndrome toxin-1 (TSST-1), produced by Staphylococcus aureus, belong to the subgroup of microbial superantigens (SAgs).
Terman David S, Park Joo Youn, Seo Keun Seok, Bohach Gregory A   +3 more
doaj   +1 more source

Putative Internal Control Genes in Bovine Milk Small Extracellular Vesicles Suitable for Normalization in Quantitative Real Time-Polymerase Chain Reaction

Membranes, 2021
Bovine milk small extracellular vesicles (sEVs) contain many biologically important molecules, including mRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used method for quantifying mRNA in tissues and cells. However, the use,
Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima   +6 more
doaj   +1 more source

qRT-PCR assays for Jamestown Canyon virus quantification.

, 2023
qRT-PCR assays for Jamestown Canyon virus quantification.
Anastacia M. Diaz (16934310), Fhallon Ware-Gilmore (11172140), Elizabeth A. McGraw (7853180), Matthew J. Jones (555280), Heverton L. C. Dutra (6714785), Meng-Jia Lau (7827683), Brianna P. McNulty (16934307)   +6 more
core   +1 more source

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

Circulating microRNA signatures of cachexia and cancer in Canis familiaris as a comparative oncology model for human disease

Molecular Oncology, EarlyView.
Circulating microRNAs as biomarkers of cachexia and sex‐specific cancer in senior dogs. In 25 client‐owned dogs, four circulating miRNAs (miR‐15a, miR‐15b, miR‐16, miR‐140) were downregulated in cachexia, with miR‐16 the strongest individual biomarker (AUC = 0.899).
Soon‐Seok Park, Kyongman An, Gyeonghwa Kim, Soo‐Nyun Choi, Hong‐Ki Lee, Keun Hur, Kyu‐Shik Jeong   +6 more
wiley   +1 more source

Differential Expression of , , and Genes in Various Adipose Tissues and Muscle from Yanbian Yellow Cattle and Yan Yellow Cattle [PDF]

Asian-Australasian Journal of Animal Sciences, 2014
The objective of this study was to investigate the correlation between cattle breeds and deposit of adipose tissues in different positions and the gene expressions of peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FASN ...
Shuang Ji, Runjun Yang, Chunyan Lu, Zhengyan Qiu, Changguo Yan, Zhihui Zhao   +5 more
doaj   +1 more source