Results 41 to 50 of about 494,714 (299)

MiR-33a Controls hMSCS Osteoblast Commitment Modulating the Yap/Taz Expression Through EGFR Signaling Regulation [PDF]

, 2019
Mesenchymal stromal cells (hMSCs) display a pleiotropic function in bone regeneration. The signaling involved in osteoblast commitment is still not completely understood, and that determines the failure of current therapies being used.
Alessandro, Riccardo, Bellavia, Daniele, Carina, Valeria, Conigliaro, Alice, Costa, Viviana, De Luca, Angela, Fini, Milena, Giavaresi, Gianluca, Raimondi, Lavinia, Salamanna, Francesca   +9 more
core   +1 more source

M gene targeted qRT-PCR approach for SARS-CoV-2 virus detection

Scientific Reports, 2023
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is the gold standard method for SARS-CoV-2 detection, and several qRT-PCR kits have been established targeting different genes of the virus.
Md. Murshed Hasan Sarkar, Showti Raheel Naser, Sanjana Fatema Chowdhury, Md. Salim Khan, Md. Ahashan Habib, Shahina Akter, Tanjina Akhtar Banu, Barna Goswami, Iffat Jahan, Maksudur Rahman Nayem, Md. Akibul Hassan, Md. Imran Khan, Mohammad Fazle Alam Rabbi, Chowdhury Rafiqul Ahsan, Md. Ibrahim Miah, Afzalun Nessa, S. M. Rashed Ul Islam, Mohammed Atiqur Rahman, Md. Aftab Ali Shaikh, Md. Sharfuddin Ahmed   +19 more
doaj   +1 more source

Experimental assessment of robust reference genes for qRT-PCR in lung cancer studies

Frontiers in Oncology, 2023
Stable internal reference genes are crucial for quantitative real-time PCR (qRT-PCR) analyses in lung cancer studies. Widely used reference genes are mostly chosen by intuition or from pan-cancer transcriptome data and lack experimental validation by qRT-
Wei Gu, Wei Gu, Yubin Wang, Ran Xu, Jiamin Li, Jingjie Jin, Jing Zhao, Yang Chen, Yuanzhi Lu, Gong Zhang   +9 more
doaj   +1 more source

Statistical calibration of qRT-PCR, microarray and RNA-Seq gene expression data with measurement error models

, 2014
The accurate quantification of gene expression levels is crucial for transcriptome study. Microarray platforms are commonly used for simultaneously interrogating thousands of genes in the past decade, and recently RNA-Seq has emerged as a promising ...
Kuczek, Thomas, Sun, Zhaonan, Zhu, Yu
core   +1 more source

Overexpression of long non-coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR-205-EGLN2 pathway. [PDF]

, 2019
Growing evidence suggests that long non-coding RNAs NORAD and miR-205 play a significant role in regulating cancer progression and metastasis. In this study, high expression of NORAD was firstly observed in melanoma tissues and human malignant melanoma ...
Cao, Ke, Chen, Xiang, Chen, Yong, Li, Jingjing, Liu, Yan, Sun, Lichun, Tang, Jintian, Wang, Aijun, Xiong, Wei, Zhou, Jianda, Zhou, Xiao   +10 more
core   +1 more source

Development of a qRT - PCR Diagnostic Kit for the Detection of FCoV

Cyprus Journal of Medical Sciences
BACKGROUND/AIMS: In recent years, feline infectious peritonitis (FIP), which led to the death of more than 3,000 cats on the island of Cyprus, has become a significant concern. FIP is a fatal disease in cats caused by feline coronavirus (FCoV).
Gülten Tuncel Dereboylu, Gökçe Akan, Melis Kalaycı, Cenk Serhan Özverel, Tamer Şanlıdağ   +4 more
doaj   +1 more source

Evaluation of the performance of a multiplex reverse transcription polymerase chain reaction kit as a potential diagnostic and surveillance kit for rotavirus in Kenya

Tropical Diseases, Travel Medicine and Vaccines, 2019
Background Diarrhea is a serious concern worldwide, especially in developing countries. Rotavirus is implicated in approximately 400,000 infant deaths annually.
Cliff Odhiambo Philip, Margaret Koech, Nancy Kipkemoi, Ronald Kirera, Janet Ndonye, Abigael Ombogo, Mary Kirui, Erick Kipkirui, Brook Danboise, Christine Hulseberg, Stacey Bateman, Alexander Flynn, Brett Swierczewski, Esther Magiri, Elizabeth Odundo   +14 more
doaj   +1 more source

Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma [PDF]

, 2016
BACKGROUND: Growing evidence is showing that metastatic cell populations are able to transfer their characteristics to less malignant cells. Exosomes (EXOs) are membrane vesicles of endocytic origin able to convey their cargo of mRNAs, microRNAs (miRs),
Bellenghi, Maria, Carè, Alessandra, Coscia, Carolina, Errico, Maria Cristina, Felicetti, Federica, Feo, Alessandra De, Pagani, Elena, Pasquini, Luca, Pedini, Francesca, Puglisi, Rossella   +9 more
core   +5 more sources

Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch. [PDF]

, 2020
Male factor infertility is a problem in today's society but many underlying causes are still unknown. The generation of a conditional Sertoli cell (SC)-specific connexin 43 (Cx43) knockout mouse line (SCCx43KO) has provided a translational model ...
Behr, Rüdiger, Brehm, Ralph HJ, Distl, Ottmar, Hilbold, Erika, Hoedemaker, Martina, Jung, Klaus, Langeheine, Marion, Metzger, Julia, Rajkovic, Aleksandar, Rode, Kristina, Wilkening, Sandra   +10 more
core   +1 more source

Single cell high-throughput qRT-PCR protocol

, 2021
Abstract Single cell high-throughput qRT-PCR protocol combines high sensitivity technique of single cell qPCR with high-throughput qPCR technology that can generate data from 96 samples and 96 genes in a single experiment. It can be adapted for various sample types- cell culture, tissue samples and extracted RNA (10 pg) and measured on ...
Sirisha Achanta, Rajanikanth Vadigepalli
openaire   +1 more source