Results 41 to 50 of about 1,161,528 (204)
Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets
Emerging Microbes and Infections, 2020 Different primers/probes sets have been developed all over the world for the nucleic acid detection of SARS-CoV-2 by quantitative real time polymerase chain reaction (qRT-PCR) as a standard method.
Xinjin Liu +13 more
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Experimental assessment of robust reference genes for qRT-PCR in lung cancer studies
Frontiers in Oncology, 2023 Stable internal reference genes are crucial for quantitative real-time PCR (qRT-PCR) analyses in lung cancer studies. Widely used reference genes are mostly chosen by intuition or from pan-cancer transcriptome data and lack experimental validation by qRT-
Wei Gu +9 more
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, 2023 Protocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
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PLoS ONE, 2020 To accurately evaluate expression levels of target genes, stable internal reference genes is required for normalization of quantitative real-time PCR (qRT-PCR) data.
Xu Su +11 more
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, 2017 qRT- PCR for each gene was carried out using a thermal cycler (Bio-Rad, Hercules, CA, USA) and amplification conditions were 40 cycles of 30s at 95°C, 3 s at 95°C, and 30 s at 60°C.
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Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
Diagnostic Pathology, 2017 Background Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where
Xiaodong Lyu +7 more
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One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples [PDF]
PeerJ, 2016 Background. Group A rotavirus (RVA) infection is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live-attenuated rotavirus vaccines, RotaTeq® and Rotarix®, has dramatically reduced RVA associated AGE and ...
Rashi Gautam +5 more
doaj +2 more sources
Membranes, 2021 Bovine milk small extracellular vesicles (sEVs) contain many biologically important molecules, including mRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used method for quantifying mRNA in tissues and cells. However, the use,
Md. Matiur Rahman +6 more
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qRT-PCR validation of RNA-seq data.
, 2021 Correlation of fold changes determined by RNA-Seq and qRT-PCR data (A). Relative expression levels of ALS1 (B) and ALS2 (C).
Yaling Geng (11356084) +8 more
core +1 more source