Results 51 to 60 of about 1,108,829 (262)
, 2016 qRT-PCR validation of differentially expressed genes in disease and normal samples of pulmonary artery for 12 genes.
Huabin Cao (2824973) +10 more
core +1 more source
Molecular Oncology, EarlyView.LUNAR is a liver‐specific long noncoding RNA (lncRNA) that is highly expressed in normal liver but becomes epigenetically silenced in hepatocellular carcinoma through promoter hypermethylation. Loss of LUNAR is associated with NOTCH activation, epithelial–mesenchymal transition, and metastasis, whereas restoring LUNAR restrains metastatic progression ...
Se Ha Jang +9 more
wiley +1 more source
Technical validation of microarray data by quantitative RT-PCR (QRT-PCR)
, 2011 Average Log2 expression of relative mRNA copy numbers derived from three replicate microarray experiments and QRT-PCR of migratory (rim) over stationary (core) glioma cells (error bars = SD).
Dominique B Hoelzinger (89231) +16 more
core +1 more source
CEACAM1 participation in breast cancer progression
Molecular Oncology, EarlyView.In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin +3 more
wiley +1 more source
Screening for qRT-PCR Internal Reference Genes in Clarias fuscus
Progress in Fishery SciencesQuantitative real-time polymerase chain reaction (qRT-PCR) is one of the most widely used molecular techniques, which allows the detection and quantification of gene expression due to its high sensitivity, specificity, and reproducibility.
Yijun SHEN +6 more
doaj +1 more source
qRT-PCR validation of the RNA-Seq data.
, 2020 The qRT-PCR data are shown as the means ± standard error (SE) of three replicates. FPKM values from the RNA-Seq analysis are shown as the means and SE of three replicates.
Jinquan Li (112721) +4 more
core +1 more source
Molecular Oncology, EarlyView.This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault +12 more
wiley +1 more source
FEBS Open Bio, EarlyView.In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara +3 more
wiley +1 more source
qRT-PCR validation of RNA-seq data.
, 2021 Correlation of fold changes determined by RNA-Seq and qRT-PCR data (A). Relative expression levels of ALS1 (B) and ALS2 (C).
Yaling Geng (11356084) +8 more
core +1 more source
Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy
FEBS Open Bio, EarlyView.This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan +11 more
wiley +1 more source