Results 51 to 60 of about 1,161,528 (204)

Standardization of Gene Expression Quantification by Absolute Real-Time qRT-PCR System Using a Single Standard for Marker and Reference Genes

Biomarker Insights, 2010
In the last decade, genome-wide gene expression data has been collected from a large number of cancer specimens. In many studies utilizing either microarray-based or knowledge-based gene expression profiling, both the validation of candidate genes and ...
Yi-Hong Zhou, Vinay R. Raj, Eric Siegel, Liping Yu   +3 more
doaj   +2 more sources

A quantitative real time PCR method to analyze T cell receptor Vβ subgroup expansion by staphylococcal superantigens

Journal of Translational Medicine, 2010
Background Staphylococcal enterotoxins (SEs), SE-like (SEl) toxins, and toxic shock syndrome toxin-1 (TSST-1), produced by Staphylococcus aureus, belong to the subgroup of microbial superantigens (SAgs).
Terman David S, Park Joo Youn, Seo Keun Seok, Bohach Gregory A   +3 more
doaj   +1 more source

Combination of conventional immunohistochemistry and qRT-PCR to detect ALK rearrangement [PDF]

Diagnostic Pathology, 2014
Compared with FISH and qRT-PCR analyses, immunohistochemistry (IHC) is the preferred screening test in most pathology practices for ALK-rearrangement detection. With 100% sensitivity and 98% specificity, the VENTANA ALK (D5F3) IHC assay has been approved in the EU and some Asian countries for ALK-rearrangement detection.
Shan, Ling, Lian, Fang, Guo, Lei, Yang, Xin, Ying, Jianming, Lin, Dongmei   +5 more
openaire   +2 more sources

qRT-PCR using standard curves.

, 2021
(A) qRT-PCR standard curves for the individual standard RNAs. The molar amount of the respective RNA fragment introduced into the qRT-PCR reaction is given on the x-axis in logarithmic scale.
Nadine Biedenkopf (2834705), Lennart Kämper (11608114), Stephan Becker (146493), Roland K. Hartmann (9320167), Marcus Lechner (618023), Paul Klemm (6262502), Jana-Christin Demper (9339743), Julia Schlereth (2834699), Simone Bach (9339740), Friedemann Weber (45518), Andreas Schoen (11608111)   +10 more
core   +1 more source

Modeling qRT-PCR dynamics with application to cancer biomarker quantification [PDF]

Statistical Methods in Medical Research, 2017
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is widely used for molecular diagnostics and evaluating prognosis in cancer. The utility of mRNA expression biomarkers relies heavily on the accuracy and precision of quantification, which is still challenging for low abundance transcripts.
Inna, Chervoneva, Boris, Freydin, Terry, Hyslop, Scott A, Waldman   +3 more
openaire   +2 more sources

qRT-PCR and sequencing.

, 2016
qRT-PCR validation of differentially expressed genes in disease and normal samples of pulmonary artery for 12 genes.
Huabin Cao (2824973), Caiying Zhang (2824976), Fei Yang (46361), Yu Zhuang (2824982), Xiaoquan Guo (2824988), Yalu Song (2824991), Tiancheng Wang (2406016), Ping Liu (156158), Huayuan Lin (2824985), Guoliang Hu (2824979), Qingyang Xiao (686993)   +10 more
core   +1 more source

qRT-PCR primers and associated genes.

, 2016
qRT-PCR primers and associated genes.
Michael A. Cotten (2634094), David L. Denlinger (555367), David S. Kang (2634097), Cheolho Sim (79085)   +3 more
core   +1 more source

Screening for qRT-PCR Internal Reference Genes in Clarias fuscus

Progress in Fishery Sciences
Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most widely used molecular techniques, which allows the detection and quantification of gene expression due to its high sensitivity, specificity, and reproducibility.
Yijun SHEN, Yian ZHU, Zhihua YANG, Minghui YE, Dayan ZHOU, Guangli LI, Changxu TIAN   +6 more
doaj   +1 more source

Covid-19 Detection using qRT-PCR - A Review

Journal for Research in Applied Sciences and Biotechnology, 2022
In the ongoing Covid pandemic, the monitoring of SARSnCoV-2 with the help of viral loads/viral kinetics has become more essential via the RT-PCR technique. However, the interpretations of qRT-PCR technique results are made as qualitative and quantitative or semi-quantitative, and day by day, this interpretation is becoming more important.
Yagyavalkya Sharma, Lalita Sharma, Priya Agrawal, Megha Gupta, Harsh Vardhan   +4 more
openaire   +1 more source

Differential Expression of , , and Genes in Various Adipose Tissues and Muscle from Yanbian Yellow Cattle and Yan Yellow Cattle [PDF]

Asian-Australasian Journal of Animal Sciences, 2014
The objective of this study was to investigate the correlation between cattle breeds and deposit of adipose tissues in different positions and the gene expressions of peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FASN ...
Shuang Ji, Runjun Yang, Chunyan Lu, Zhengyan Qiu, Changguo Yan, Zhihui Zhao   +5 more
doaj   +1 more source