Results 51 to 60 of about 494,714 (299)

Putative Internal Control Genes in Bovine Milk Small Extracellular Vesicles Suitable for Normalization in Quantitative Real Time-Polymerase Chain Reaction

Membranes, 2021
Bovine milk small extracellular vesicles (sEVs) contain many biologically important molecules, including mRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used method for quantifying mRNA in tissues and cells. However, the use,
Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima   +6 more
doaj   +1 more source

Deficiency of Capicua disrupts bile acid homeostasis [PDF]

, 2018
Capicua (CIC) has been implicated in pathogenesis of spinocerebellar ataxia type 1 and cancer in mammals; however, the in vivo physiological functions of CIC remain largely unknown.
Choi, N, Fryer, JD, Hwang, D, Jung, HY, Kang, H, Kim, E, KIM, KYONG TAI, Kim, S, Lee, J, LEE, YOONTAE, Park, S, Yoe, J, Zoghbi, HY   +12 more
core   +2 more sources

Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay

Diagnostic Pathology, 2017
Background Fusion genes generated from chromosomal translocation play an important role in hematological malignancies. Detection of fusion genes currently employ use of either conventional RT-PCR methods or fluorescent in situ hybridization (FISH), where
Xiaodong Lyu, Xianwei Wang, Lina Zhang, Zhenzhu Chen, Yu Zhao, Jieying Hu, Ruihua Fan, Yongping Song   +7 more
doaj   +1 more source

One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples [PDF]

PeerJ, 2016
Background. Group A rotavirus (RVA) infection is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live-attenuated rotavirus vaccines, RotaTeq® and Rotarix®, has dramatically reduced RVA associated AGE and ...
Rashi Gautam, Slavica Mijatovic-Rustempasic, Mathew D. Esona, Ka Ian Tam, Osbourne Quaye, Michael D. Bowen   +5 more
doaj   +2 more sources

Identification of a Proliferation Gene Cluster Associated with HPV E6/E7 Expression Level and Viral DNA Load in Invasive Cervical Carcinoma

, 2005
Specific HPV DNA sequences are associated with more than 90% of invasive carcinomas of the uterine cervix. Viral E6 and E7 oncogenes are key mediators in cell transformation by disrupting TP53 and RB pathways. To investigate molecular mechanisms involved
Anne de La Rochefordière, Christophe Rosty, Dafna Tsafrir, Eytan Domany, F Ferlay, François Radvanyi, Ilan Tsafrir, Jean Paul Thiery, Jérôme Couturier, Martine Peter, Michal Sheffer, Nicolas Stransky, Patricia de Crémoux, Rémy Salmon, Thierry Dorval, Xavier Sastre-Garau   +15 more
core   +1 more source

Circ-ZNF609 regulates G1-S progression in rhabdomyosarcoma [PDF]

, 2019
Circular RNAs (circRNAs) represent a class of covalently closed RNAs, derived from non-canonical splicing events, which are expressed in all eukaryotes and often conserved among different species.
Bozzoni, Irene, Colantoni, Alessio, Dattilo, Dario, DI TIMOTEO, Gaia, Dominici, Carlo, Legnini, Ivano, Megiorni, Francesca, Morlando, Mariangela, Rossi, Francesca, Santini, Tiziana   +9 more
core   +1 more source

qRT-PCR sample preparation v1

, 2023
Protocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
openaire   +1 more source

Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.

PLoS ONE, 2020
To accurately evaluate expression levels of target genes, stable internal reference genes is required for normalization of quantitative real-time PCR (qRT-PCR) data.
Xu Su, Liuyang Lu, Yashe Li, Congai Zhen, Guilei Hu, Kun Jiang, Yawei Yan, Yanbo Xu, Geng Wang, Mingwang Shi, Xiling Chen, Baizhong Zhang   +11 more
doaj   +1 more source

Protocol for the assessment of viral retention capability of membranes [PDF]

, 2011
A series of experiments has been carried out to determine the Log removal value (LRV) of MS2 bacteriophages suspended in various buffers (osmosed water, tap water, aqueous solutions of NaCl and phosphate buffer solution) during filtration through hollow ...
Aimar, Pierre, Bergé, Mathieu, Causserand, Christel, Causserand, Christel, Furiga, Aurélie, Pierre, Gwenaelle, Roques, Christine   +6 more
core   +2 more sources

Targeted modulation of IGFL2‐AS1 reveals its translational potential in cervical adenocarcinoma

Molecular Oncology, EarlyView.
Cervical adenocarcinoma patients face worse outcomes than squamous cell carcinoma counterparts despite similar treatment. The identification of IGFL2‐AS1's differential expression provides a molecular basis for distinguishing these histotypes, paving the way for personalized therapies and improved survival in vulnerable populations globally.
Ricardo Cesar Cintra, Lucca Paolo Hsu Helmich, Daniel Rodrigues de Bastos, Laura Sichero, Patrícia Savio de Araujo‐Souza, Fabio Passetti, Luisa Lina Villa   +6 more
wiley   +1 more source