Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis [PDF]
Frontiers in Genetics, 2020 Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis.
Yao Lv +9 more
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Reference Gene Selection for Quantitative Gene Expression Analysis in Argynnis hyperbius [PDF]
InsectsArgynnis hyperbius (Lepidoptera: Nymphalidae), as an important environmental indicator species, has shown a gradual decline in its species richness amid intensifying climate change and increasing environmental pressures.
Hong-Juan Xin +6 more
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qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Litsea coreana) [PDF]
Current Issues in Molecular BiologyTo identify stable reference genes for qRT-PCR analysis across different developmental stages and color variations of tender leaves in Litsea coreana, seven candidate reference genes were selected based on existing transcriptome data.
Qianli Dai +10 more
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EF1α and αTUB Are Stable Reference Gene Pairs for RT-qPCR-Based Gene Expression Studies in Salix suchowensis Under Nitrogen Treatment Conditions [PDF]
PlantsSalix suchowensis is an ideal model organism for investigating nitrogen (N) transport mechanisms due to its low N-input requirements. Accurate quantification of gene expression is essential for elucidating these processes, with quantitative real-time PCR
Lei Huang +8 more
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PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic–pituitary–gonadal axis in sows [PDF]
Animal Bioscience, 2022 Objective The quantitative reverse transcription polymerase chain reaction (qPCR) is the most accurate and reliable technique for analysis of gene expression.
Hwan-Deuk Kim +10 more
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iRGvalid: A Robust in silico Method for Optimal Reference Gene Validation
Frontiers in Genetics, 2021 BackgroundAppropriate reference genes are critical to accurately quantifying relative gene expression in research and clinical applications. Numerous efforts have been made to select the most stable reference gene(s), but a consensus has yet to be ...
Zhongxu Zhu, Keqin Gregg, Wenli Zhou
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The Gene Ontology's Reference Genome Project: a unified framework for functional annotation across species. [PDF]
PLoS Computational Biology, 2009 The Gene Ontology (GO) is a collaborative effort that provides structured vocabularies for annotating the molecular function, biological role, and cellular location of gene products in a highly systematic way and in a species-neutral manner with the aim ...
Reference Genome Group of the Gene Ontology Consortium
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Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]
BMC Genomics, 2019 Abstract Background RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before comparing expression levels.
Karen Cristine Gonçalves Dos Santos +2 more
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Infectious Agents and Cancer, 2022 Background Sexually transmitted infections (STIs) are prevalent throughout the world and impose a significant burden on individual health and public health systems.
Zhihai Ma +12 more
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Reference genes in real-time PCR [PDF]
Journal of Applied Genetics, 2013 This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Kozera, Bartłomiej, Rapacz, Marcin
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