Results 1 to 10 of about 490,106 (140)

Real time polymerase chain reaction for detecting SARS-COV-2 in Indonesia: are the results reliable?

Universa Medicina, 2020
Since it was first reported in Wuhan, China in December 2019, coronavirus disease 2019 (COVID-19) has been considered as a pandemic. It is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a member of the Coronavirus family.
Monica Dwi Hartanti
doaj   +1 more source

Reference gene selection for quantitative real-time polymerase chain reaction analysis [PDF]

Invertebrate Survival Journal, 2017
Bombyx mori nucleopolyhedrovirus (BmNPV) is the most serious viral disease in silkworms. To investigate the mechanisms of the immune responses of B.
Z Nie, P Lu, X Chen, Q Wang, X Meng, S Lu, X Dong, K Chen   +7 more
doaj  

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Emerging Infectious Diseases, 2004
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV).
Shannon L. Emery, Dean D. Erdman, Michael D. Bowen, Bruce R. Newton, Jonas M. Winchell, Richard F. Meyer, Suxiang Tong, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Paul A. Rota, Bettina Bankamp, Luis E. Lowe, Tom G. Ksiazek, William J. Bellini, Larry J. Anderson   +15 more
doaj   +1 more source

Real-time Polymerase Chain Reaction in the Diagnosis of Acute Postoperative Endophthalmitis [PDF]

American Journal of Ophthalmology, 2012
To evaluate the efficacy of quantitative real-time polymerase chain reaction (qPCR) in the diagnosis of postoperative bacterial endophthalmitis among patients who underwent cataract surgery at a tertiary care center.Prospective experimental study.This was a single-center study of 64 eyes of 64 patients presenting with clinical signs and symptoms of ...
Cornelia Reena, Joseph, Prajna, Lalitha, Kavitha R, Sivaraman, Kim, Ramasamy, Umesh Chandra, Behera   +4 more
openaire   +4 more sources

Real Time Polymerase Chain Reaction : Perangkat Diagnostic Alternatif untuk Melacak Virus Nipah (REAL TIME POLYMERASE CHAIN REACTION : AN ALTERNATIVE DIAGNOSTIC TOOL TO DETECT NIPAH VIRUS)

Jurnal Veteriner, 2014
Nipah is a dangerous zoonotic disease with a high social, economical and psychological impact. Fruitbat Pteropus sp. is one of the nipah virus  reservoir host.
Indrawati Sendow, Atik Ratnawati, Raden Mas Abdul Adjid, Muharam Saepulloh   +3 more
doaj  

New Method for Differentiation of Granuloviruses (Betabaculoviruses) Based on Real-Time Polymerase Chain Reaction (Real-Time PCR)

Viruses, 2019
Baculoviridae is a highly diverse family of rod-shaped viruses with double-stranded DNA. To date, almost 100 species have had their complete genomic sequences deposited in the GenBank database, a quarter of which comprises granuloviruses (GVs).
Martyna Krejmer-Rabalska, Lukasz Rabalski, Michael D. Jukes, Marlinda Lobo de Souza, Sean D. Moore, Boguslaw Szewczyk   +5 more
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Development of a real time polymerase chain reaction for quantitation of Schistosoma mansoni DNA

Memorias do Instituto Oswaldo Cruz, 2006
This report describes the development of a SYBR Green I based real time polymerase chain reaction (PCR) protocol for detection on the ABI Prism 7000 instrument.
Ana Lisa do Vale Gomes, Fábio L Melo, Roberto P Werkhauser, Frederico GC Abath   +3 more
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Detection and quantification in real-time polymerase chain reaction [PDF]

, 2013
The estimation of the concentration of an infectious agent in the environment is a key step to trigger an alert when there is a bio- logical threat. This concentration can be obtained trough a quantitative polymerase chain reaction (qPCR). Nevertheless, standard real-time pro- cedure do not address detection delay which is a main concern in alert ...
Keita, Abou, Hérault, Romain, Calbrix, Colas, Canu, Stéphane   +3 more
openaire   +1 more source

Performance of Robust Regression Methods in Real-Time Polymerase Chain Reaction Calibration

The International Journal of Biological Markers, 2014
The ordinary least squares (OLS) method is routinely used to estimate the unknown concentration of nucleic acids in a given solution by means of calibration.
Annalisa Orenti, Ettore Marubini
doaj   +1 more source

Detection of Porcine DNA in Processed Beef Products Using Real Time – Polymerase Chain Reaction

Indonesian Journal of Halal Research, 2019
Meat is one of food materials which has protein source and mostlyconsumed by non-vegetarian. Consuming halal food is an obligation for every Muslim. Meat processed products usually contaminated by pork.
Triayu Septiani
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