Results 41 to 50 of about 490,106 (140)

A kinetic model of quantitative real‐time polymerase chain reaction [PDF]

open access: yesBiotechnology and Bioengineering, 2005
AbstractReal‐time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined.
Sarika, Mehra, Wei-Shou, Hu
openaire   +2 more sources

Problem‐solving test: Real‐time polymerase chain reaction [PDF]

open access: yesBiochemistry and Molecular Biology Education, 2009
AbstractTerms to be familiar with before you start to solve the test: polymerase chain reaction, DNA amplification, electrophoresis, breast cancer, HER2 gene, genomic DNA, in vitro DNA synthesis, template, primer, Taq polymerase, 5′→3′ elongation activity, 5′→3′ exonuclease activity, deoxyribonucleoside triphosphates, DNA structure, proofreading ...
openaire   +2 more sources

Real-time polymerase chain reaction for diagnosis and management of HIV-induced uveitis

open access: yesIndian Journal of Ophthalmology, 2018
Intraocular (IO) inflammation in patients with Human immune deficiency virus (HIV) infection can be due to opportunistic infections, immune recovery uveitis, drugs used in the management or a primary manifestation of HIV itself. We studied the role of RT-
Jayapratha R Selvaraj   +6 more
doaj   +1 more source

DNA extraction and polymerase chain reaction

open access: yesJournal of Cytology, 2019
DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
doaj   +1 more source

A comparison of three diagnostic platforms for the detection of influenza A and B in children

open access: yesBiomedical and Biotechnology Research Journal, 2018
Background: Viral flu is the predominant cause of hospitalization in young children, which invariably leads to enhanced morbidity and mortality in children in developing countries. Initial treatment of viral flu is based on presumptive diagnosis. Bedside
Furqan Kabir   +6 more
doaj   +1 more source

Diagnosed tuberculous meningitis using cerebrospinal fluid polymerase chain reaction in patients hospitalized with the diagnosis of meningitis in referral hospitals in Isfahan

open access: yesJournal of Research in Medical Sciences, 2015
Background: Tuberculosis (TB) remains one of the leading infectious diseases throughout the world. Among various forms of extrapulmonary TB, tuberculous meningitis (TBM) is the most severe form and remains a major global health problem with a high ...
Kiana Shirani   +5 more
doaj  

The Effect of Resveratrol on Expression of microRNA34a in U87MG Cancerous Cells Line

open access: yesمجله دانشکده پزشکی اصفهان, 2017
Background: Glioblastoma multiform (GBM) is known as the most current and invasive kind of central nervous system tumors. In addition to existing therapy methods, using epigenetic factors specially investigation of microRNAs expression level has been ...
Narges Shahsavani, Mahta Mazaheri
doaj  

real-time polymerase chain reaction

open access: yes
Citation: 'real-time polymerase chain reaction' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.09720 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms ...
openaire   +1 more source

A rapid low-cost real-time PCR for the detection of klebsiella pneumonia carbapenemase genes

open access: yesAnnals of Clinical Microbiology and Antimicrobials, 2012
Background Klebsiella pneumonia carbapenemases (KPCs) are able to hydrolyze the carbapenems, which cause many bacteria resistance to multiple classes of antibiotics, so the rapid dissemination of KPCs is worrisome.
Wang Lijun, Gu Haitong, Lu Xinxin
doaj   +1 more source

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