Results 51 to 60 of about 731,111 (296)

Identification of Exiguobacterium acetylicum by a double real- time polymerase chain reaction assay

浙江大学学报. 农业与生命科学版, 2017
Exiguobacterium sp. has been figured out to be the main spoilage bacteria on many kinds of food, and it has the potential to be the main spoiler. Bacteria Exiguobacterium have already been isolated from extreme environment and they have evolved and ...
GAO Cancan, LIU Jiamei, LI Junjie, LU Zhaoxin, LÜ Fengxia, ZHANG Chong, ZHAO Haizhen, BIE Xiaomei   +7 more
doaj   +1 more source

Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives [PDF]

, 2015
This is a final draft version of the publication following acceptance by the journal.Quantitative polymerase chain reaction (qPCR) is a method of amplifying and detecting small samples of genetic material in real time and is in routine use across many ...
Karteris, E, Rogers-Broadway, KR
core   +1 more source

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

Data representing applicability of developed growth hormone 1 (GH1) gene detection method for detecting Atlantic salmon (Salmo salar) at high specificity to processed salmon commodities

Data in Brief, 2019
This article is referred to the research article entitled “Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction” by Soga et al.
Keisuke Soga, Kosuke Nakamura, Takumi Ishigaki, Shinya Kimata, Kiyomi Ohmori, Masahiro Kishine, Junichi Mano, Reona Takabatake, Kazumi Kitta, Hiroyuki Nagoya, Kazunari Kondo   +10 more
doaj   +1 more source

Real time polymerase chain reaction for detecting SARS-COV-2 in Indonesia: are the results reliable?

Universa Medicina, 2020
Since it was first reported in Wuhan, China in December 2019, coronavirus disease 2019 (COVID-19) has been considered as a pandemic. It is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a member of the Coronavirus family.
Monica Dwi Hartanti
doaj   +1 more source

Clinical profile of children with influenza a and b infections admitted to a tertiary care hospital in South India

International Journal of Advanced Medical and Health Research, 2021
Background: The influenza pandemics have caused serious morbidity and mortality around the world. Only a few studies have described the clinical profile of both influenza A and B infections among children and its seasonal variations.
Janani Arul, Satheesh Ponnarmeni, Sharmila Ferdinamarie, Rahul Dhodapkar, Peter Prasanth Kumar Kommu   +4 more
doaj   +1 more source

ERM®-AD623: Certified Reference Material for the Quantification of BCR-ABL1 Transcripts [PDF]

, 2015
The JRC’s Institute for Reference Materials and Measurements (IRMM) has released the certified reference material (CRM) ERM-AD623 for the calibration of quantitative real time polymerase chain reaction (qPCR) assays measuring the BCR-ABL1 transcript ...
DEPREZ LIESBET
core  

Colorectal cancer‐derived FGF19 is a metabolically active serum biomarker that exerts enteroendocrine effects on mouse liver

Molecular Oncology, EarlyView.
Meta‐transcriptome analysis identified FGF19 as a peptide enteroendocrine hormone associated with colorectal cancer prognosis. In vivo xenograft models showed release of FGF19 into the blood at levels that correlated with tumor volumes. Tumoral‐FGF19 altered murine liver metabolism through FGFR4, thereby reducing bile acid synthesis and increasing ...
Jordan M. Beardsley, Michael W. Rohr, Joseph A. Goode, Sai Preethi Nakkina, Jignesh G. Parikh, Deborah A. Altomare   +5 more
wiley   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Emerging Infectious Diseases, 2004
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV).
Shannon L. Emery, Dean D. Erdman, Michael D. Bowen, Bruce R. Newton, Jonas M. Winchell, Richard F. Meyer, Suxiang Tong, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Paul A. Rota, Bettina Bankamp, Luis E. Lowe, Tom G. Ksiazek, William J. Bellini, Larry J. Anderson   +15 more
doaj   +1 more source