Results 31 to 40 of about 490,106 (140)

Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003

Emerging Infectious Diseases, 2004
During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated ...
Junhui Zhai, Thomas Briese, Erhei Dai, Xiaoyi Wang, Xin Pang, Zongmin Du, Haihong Liu, Jin Wang, Hongxia Wang, Zhaobiao Guo, Zeliang Chen, Lingxiao Jiang, Dongsheng Zhou, Yanping Han, Omar Jabado, Gustavo Palacios, W. Ian Lipkin, Ruifu Yang   +17 more
doaj   +1 more source

Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia

Revista Brasileira de Hematologia e Hemoterapia, 2015
Introduction: Minimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia.
Francisco Danilo Ferreira Paula, Silvana Maria Elói-Santos, Sandra Guerra Xavier, Mônica Aparecida Ganazza, Patricia Yoshioka Jotta, José Andrés Yunes, Marcos Borato Viana, Juliana Godoy Assumpção   +7 more
doaj   +1 more source

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains [PDF]

Archives of Razi Institute, 2019
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents.
S. Alamian, T. Zahraei Salehi, K. Aghaiypour Kolyani, M. Esmaelizad, A. Etemadi   +4 more
doaj   +1 more source

Performance and Cost-effectiveness of Pooling COVID-19 Samples Using Real time Polymerase Chain Reaction Test

Journal of Nepal Health Research Council, 2022
Background: Rapid detection of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) by real-time polymerase chain reaction (RT-PCR) is the most reliable method used worldwide.
Ira Shrestha, Shreekrishna Maharjan, Shital Bhandary, Sunita Prajapati, Ajay Chaurasiya, Rojina Bakhunchhe   +5 more
doaj   +1 more source

Real-Time Polymerase Chain Reaction Detection of Fishmeal in Feedstuffs [PDF]

Journal of AOAC INTERNATIONAL, 2010
Abstract A SYBR<sup/> Green PCR system was developed for detection of fishmeal in feedstuffs. The real-time PCR method combines the use of fish-specific primers that amplify an 87 base pair (bp) fragment of the mitochondrial 12S ribosomal RNA gene from fish species, and a positive control primer pair that amplifies a 99 bp ...
Irene, Martín, Teresa, García, Maria, Rojas, Nicolette, Pegels, Miguel Angel, Pavón, Pablo E, Hernández, Isabel, González, Rosario, Martín   +7 more
openaire   +2 more sources

Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction

Вопросы вирусологии, 2020
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko, Svetlana V. Koteneva, Tatyana I. Glotova, Alexander G. Glotov   +3 more
doaj   +1 more source

Clinical profile of children with influenza a and b infections admitted to a tertiary care hospital in South India

International Journal of Advanced Medical and Health Research, 2021
Background: The influenza pandemics have caused serious morbidity and mortality around the world. Only a few studies have described the clinical profile of both influenza A and B infections among children and its seasonal variations.
Janani Arul, Satheesh Ponnarmeni, Sharmila Ferdinamarie, Rahul Dhodapkar, Peter Prasanth Kumar Kommu   +4 more
doaj   +1 more source

Multiplex Real-Time Polymerase Chain Reaction on Sputum for the Diagnosis of Pneumocystis jirovecii Pneumonia in Children: A Retrospective Study

Infection and Drug Resistance, 2021
Juan Jiang,1,2 Xia Wang,3 Jian He,3 Donglei Liao,3 Xiaolu Deng3 1Department of Respiratory Medicine (National Key Clinical Specialty), Xiangya Hospital, Central South University, Changsha, People’s Republic of China; 2Hunan Provincial Clinical Research ...
Jiang J, Wang X, He J, Liao D, Deng X
doaj  

Identification of Exiguobacterium acetylicum by a double real- time polymerase chain reaction assay

浙江大学学报. 农业与生命科学版, 2017
Exiguobacterium sp. has been figured out to be the main spoilage bacteria on many kinds of food, and it has the potential to be the main spoiler. Bacteria Exiguobacterium have already been isolated from extreme environment and they have evolved and ...
GAO Cancan, LIU Jiamei, LI Junjie, LU Zhaoxin, LÜ Fengxia, ZHANG Chong, ZHAO Haizhen, BIE Xiaomei   +7 more
doaj   +1 more source

Data representing applicability of developed growth hormone 1 (GH1) gene detection method for detecting Atlantic salmon (Salmo salar) at high specificity to processed salmon commodities

Data in Brief, 2019
This article is referred to the research article entitled “Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction” by Soga et al.
Keisuke Soga, Kosuke Nakamura, Takumi Ishigaki, Shinya Kimata, Kiyomi Ohmori, Masahiro Kishine, Junichi Mano, Reona Takabatake, Kazumi Kitta, Hiroyuki Nagoya, Kazunari Kondo   +10 more
doaj   +1 more source