Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction [PDF]
Journal of Computational Biology, 2005 Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful data from this method depend on fitting data to theoretical curves that allow computation of mRNA levels.
Sheng, Zhao, Russell D, Fernald
openaire +4 more sources
On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]
, 2007 We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core +1 more source
Revista Panamericana de Salud Pública, 2022 Objective. To evaluate molecular tools to detect low-level parasitemia and the five species of Plasmodium that infect humans for use in control and elimination programs, and in reference laboratories. Methods. We evaluated 145 blood samples from patients
Mariana Aschar +8 more
doaj +1 more source
A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test [PDF]
, 2014 A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species.
De Craeye, Stéphane +6 more
core +7 more sources
Comparison of the direct fluorescence assay and real-time polymerase chain reaction for the detection of influenza virus A and B in immunocompromised patients [PDF]
, 2013 OBJECTIVE: This study evaluated the diagnostic performance of two methods for the detection of influenza virus in immunocompromised transplant patients.
Bellei, Nancy Cristina Junqueira +5 more
core +4 more sources
Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction [PDF]
, 2012 We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively.
Ballhause, M. +16 more
core +5 more sources
Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction
Taiwanese Journal of Obstetrics & Gynecology, 2009 Objective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation ...
Horng-Jyh Tsai, MD, PhD, FACOG
doaj +3 more sources
Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]
, 2011 Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els +3 more
core +3 more sources
Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction [PDF]
, 2005 Aim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.
Chen, Y +9 more
core +1 more source
Downregulation of plasma MiR-142-3p and MiR-26a-5p in patients with colorectal carcinoma [PDF]
, 2015 Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy.
Asadi, J. +9 more
core +1 more source