Results 21 to 30 of about 490,106 (140)

Ultrasensitive molecular tests for Plasmodium detection: applicability in control and elimination programs and reference laboratories

open access: yesRevista Panamericana de Salud Pública, 2022
Objective. To evaluate molecular tools to detect low-level parasitemia and the five species of Plasmodium that infect humans for use in control and elimination programs, and in reference laboratories. Methods. We evaluated 145 blood samples from patients
Mariana Aschar   +8 more
doaj   +1 more source

Prenatal Group B Streptococcus Test Using Real-Time Polymerase Chain Reaction

open access: yesTaiwanese Journal of Obstetrics & Gynecology, 2009
Objective: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation ...
Chi-Feng Wei   +7 more
doaj   +3 more sources

Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction [PDF]

open access: yesJournal of Computational Biology, 2005
Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful data from this method depend on fitting data to theoretical curves that allow computation of mRNA levels.
Sheng Zhao, Russell D. Fernald
openaire   +2 more sources

Real-time polymerase chain reaction in recurrent cytomegalovirus anterior uveitis

open access: yesAmerican Journal of Ophthalmology Case Reports, 2019
Purpose: Cytomegalovirus (CMV) has been reported to cause anterior uveitis in the immunocompetent people. Recurrence of this viral uveitis poses a mangement dilemma.
Vinita Girish Rao   +2 more
doaj   +1 more source

DETECTION OF CAMPYLOBACTER SPP. WITH REAL-TIME POLYMERASE CHAIN REACTION

open access: yesВетеринария сегодня, 2019
Bacteria of Campylobacter genus are ones of the main zoonotic pathogens causing human and animal diseases. Campylobacter organisms are microaerophiles and, therefore, require low oxygen concentration (3–5%) and high carbon dioxide concentration (3–10 ...
G. S. Skitovich   +3 more
doaj   +1 more source

Identification of Cronobacter in infant formula using real-time polymerase chain reaction

open access: yesZhongguo shipin weisheng zazhi, 2023
ObjectiveTo establish a real-time quantitative polymerase chain reaction (PCR) method for rapid and accurate identification of Cronobacter spp. in food samples and artificially contaminated samples.MethodsPrimers and probes were designed based on the ...
WANG Qinglong   +10 more
doaj   +1 more source

Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2018
Introduction: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum.
Rubén E. Varela-M   +2 more
doaj   +1 more source

Low-Cost, Real-Time Polymerase Chain Reaction System with Integrated RNA Extraction

open access: yesSensors, 2023
Rapid, easy-to-use, and low-cost systems for biological sample testing are important for point-of-care diagnostics and various other health applications.
Tchamie Kadja   +2 more
doaj   +1 more source

Real-time polymerase chain reaction in acute retinal necrosis following encephalitis

open access: yesIndian Journal of Ophthalmology, 2018
Acute retinal necrosis (ARN) following herpes simplex encephalitis (HSE) in an immunocompetent patient is a rare condition. Quantitative real-time polymerase chain reaction (qPCR) has made it possible to identify and quantify viral genome.
Vinita Rao   +2 more
doaj   +1 more source

Rapid and accurate diagnosis of infection by real-time polymerase chain reaction [PDF]

open access: yesIntestinal Research, 2018
Background/AimsThe incidence and severity of Clostridium difficile infection (CDI) have increased worldwide, resulting in a need for rapid and accurate diagnostic methods.MethodsA retrospective study was conducted to compare CDI diagnosis methods between
Pil Hun Song   +9 more
doaj   +1 more source

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