Results 11 to 20 of about 8,117,731 (311)
RefPrimeCouch—a reference gene primer CouchApp [PDF]
Database, 2013 To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive.
Silbermann, Jascha +3 more
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Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]
BMC Genomics, 2019 Abstract Background RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before comparing expression levels.
Karen Cristine Gonçalves Dos Santos +2 more
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Reference Genes for the Normalization of Gene Expression in Eucalyptus Species [PDF]
Plant and Cell Physiology, 2011 Gene expression analysis is increasingly important in biological research, with reverse transcription-quantitative PCR (RT-qPCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Considering the increased sensitivity, reproducibility and large dynamic range of this method, the requirements for proper
Luisa Abruzzi, de Oliveira +6 more
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Reference genes for gene expression studies in the mouse heart [PDF]
Scientific Reports, 2017 AbstractTo be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field.
Ruiz-Villalba, Adrián +5 more
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Reference genes for measuring mRNA expression [PDF]
Theory in Biosciences, 2012 The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature.
Jitesh Dundas, Maurice H. T. Ling
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Insects, 2021 Bactrocera dorsalis is a major pest that causes serious damage to many fruits. Although phytosanitary treatment methods have been developed for Bactrocera control, there is a lack of information related to the gene expression pattern of B.
Yue Cao +5 more
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Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis
Frontiers in Genetics, 2020 Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis.
Yao Lv +9 more
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Identification of stable senescence‐associated reference genes [PDF]
Aging Cell, 2019 AbstractCellular senescence is a state of permanent cell cycle arrest activated in response to damaging stimuli. Many hallmarks associated with senescent cells are measured by quantitative real‐time PCR (qPCR). As the selection of stable reference genes for interpretation of qPCR data is often overlooked, we performed a systematic review to understand ...
Alejandra Hernandez‐Segura +2 more
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Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)
Insects, 2022 Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) is a widely used tool for measuring gene expression; however, its accuracy relies on normalizing the data to one or more stable reference genes.
Ying-Na Pan +6 more
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Applied Sciences, 2022 Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a powerful technique for studying gene expression. The key to quantitative accuracy depends on the stability of the reference genes used for data normalization under different ...
Yajing Wu +7 more
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