Results 11 to 20 of about 7,761,125 (307)

RefPrimeCouch—a reference gene primer CouchApp [PDF]

Database, 2013
To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive.
Silbermann, Jascha, Wernicke, Catrin, Pospisil, Heike, Frohme, Marcus   +3 more
openaire   +4 more sources

Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]

BMC Genomics, 2019
Abstract Background RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before comparing expression levels.
Karen Cristine Gonçalves Dos Santos, Isabel Desgagné-Penix, Hugo Germain   +2 more
openaire   +4 more sources

Reference genes for gene expression studies in the mouse heart [PDF]

Scientific Reports, 2017
AbstractTo be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field.
Ruiz-Villalba, Adrián, Mattiotti, Andrea, Gunst, Quinn D, Cano-Ballesteros, Sara, Van, Maurice JB, Ruijter, JM   +5 more
openaire   +6 more sources

Reference Genes for the Normalization of Gene Expression in Eucalyptus Species [PDF]

Plant and Cell Physiology, 2011
Gene expression analysis is increasingly important in biological research, with reverse transcription-quantitative PCR (RT-qPCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Considering the increased sensitivity, reproducibility and large dynamic range of this method, the requirements for proper
Luisa Abruzzi, de Oliveira, Michèle Claire, Breton, Fernanda Macedo, Bastolla, Sandro da Silva, Camargo, Rogério, Margis, Jeverson, Frazzon, Giancarlo, Pasquali   +6 more
openaire   +2 more sources

Real-Time Quantitative PCR: Primer Design, Reference Gene Selection, Calculations and Statistics. [PDF]

Metabolites, 2023
Real-time quantitative PCR is a technique that can measure the content of the target nucleic acid sequence of interest in a given sample. It is mainly divided into absolute and relative quantitative methods.
Chen Z, Halford NG, Liu C.
europepmc   +2 more sources

Reference genes in real-time PCR [PDF]

Journal of Applied Genetics, 2013
This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Bartłomiej Kozera, Marcin Rapacz
openaire   +2 more sources

Reference genes for measuring mRNA expression [PDF]

Theory in Biosciences, 2012
The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature.
Jitesh Dundas, Maurice H. T. Ling
openaire   +4 more sources

Identification of stable senescence‐associated reference genes [PDF]

Aging Cell, 2019
AbstractCellular senescence is a state of permanent cell cycle arrest activated in response to damaging stimuli. Many hallmarks associated with senescent cells are measured by quantitative real‐time PCR (qPCR). As the selection of stable reference genes for interpretation of qPCR data is often overlooked, we performed a systematic review to understand ...
Alejandra Hernandez‐Segura, Richard Rubingh, Marco Demaria   +2 more
openaire   +2 more sources

Reference materials (RMs) for analysis of the human factor II (prothrombin) gene G20210A mutation [PDF]

, 2005
The Scientific Committee of Molecular Biology Techniques (C-MbT) in Clinical Chemistry of the IFCC has initiated a joint project in co-operation with the European Commission, Joint Research Centre, Institute of Reference Materials and Measurements to ...
Tosetto, Alberto, Visvikis, Sophie, RIEGER Sandra, GANCBERG D, Kitchen, Steve, MARKI-ZAY Janos, MANNHALTER C., Schimmel, Heinz G., VISVIKIS S, Gancberg, David, CORBISIER P, REITSMA Pieter, KITCHEN S, TORDAI A, Rieger, Sandra, Halbmayer, Walter-Michael, REITSMA PH, SPANNAGL M, GANCBERG David, Tordai, Attilia, Opplinger Leibundgut, Elisabeth, SPANNAGL Michael, OPPLIGER LEIBUNDGUT E, NEUMAIER Michael, Oppliger Leibundgut, Elisabeth, MELEGH B, CORBISIER Philippe, OLDENBURG Johannes, RIEGER S, Corbisier, Philippe, OLDENBURG J, COOPER S, Leibundgut, Elisabeth Oppliger, HALBMAYER Walter-Michael, Cooper, Susan, HALBMAYER WM, COOPER Susan, KITCHEN Steve, MRKI ZAY J, Reitsma, Pieter H., Marki-Zay, János, Gemmati, Donato, TOSETTO A, VISVIKIS Sophie, TORDAI Attila, Melegh, Béla, Zadro, Renata, SCHIMMEL HG, Neumaier, Michael, KLEIN Christoph, SCHIMMEL Heinz, TOSETTO Alberto, Oldenburg, Johannes, MANNHALTER Christine, ZADRO R, Mannhalter, Christine, Klein, Christoph L., MELEGH Bela, NEUMAIER M, Spannagl, Michael, KLEIN CL, OPPLIGER LEIBUNDGUT Elisabeth, Cooper, S., GEMMATI Donato, ZADRO Renata   +64 more
core   +2 more sources

With Reference to Reference Genes: A Systematic Review of Endogenous Controls in Gene Expression Studies

PLOS ONE, 2015
The choice of reference genes that are stably expressed amongst treatment groups is a crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have specified that a minimum of two validated reference genes should be used for normalisation.
Joanne R Chapman, Jonas Waldenström
openaire   +4 more sources