Results 11 to 20 of about 2,311,393 (299)
RefPrimeCouch—a reference gene primer CouchApp [PDF]
Database, 2013 To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive.
Silbermann, Jascha +3 more
openaire +3 more sources
Insects, 2021 The tomato leaf miner, Tuta absoluta is a destructive pest of tomato. The leaf-mining activities of its larvae can cause significant yield losses. Real-time quantitative polymerase chain reaction (RT-qPCR) is commonly used to measure gene expression, and
Xin Yan +4 more
doaj +1 more source
Reference genes for measuring mRNA expression [PDF]
Theory in Biosciences, 2012 The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature.
Dundas, J, Ling, M
openaire +3 more sources
Identification of stable senescence‐associated reference genes [PDF]
Aging Cell, 2019 AbstractCellular senescence is a state of permanent cell cycle arrest activated in response to damaging stimuli. Many hallmarks associated with senescent cells are measured by quantitative real‐time PCR (qPCR). As the selection of stable reference genes for interpretation of qPCR data is often overlooked, we performed a systematic review to understand ...
Alejandra Hernandez‐Segura +2 more
openaire +2 more sources
Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes
Frontiers in Physiology, 2021 Reverse transcription quantitative real-time PCR is the most commonly used method to detect gene expression levels. In experiments, it is often necessary to correct and standardize the expression level of target genes with reference genes.
Wei Na +24 more
doaj +1 more source
Reference-based QUantification Of gene Dispensability (QUOD) [PDF]
Plant Methods, 2020 Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level.
Frey, Katharina +2 more
openaire +5 more sources
Reference genes for gene expression studies in the mouse heart [PDF]
Scientific Reports, 2017 AbstractTo be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field.
Ruiz-Villalba, Adrián +5 more
openaire +6 more sources
Animals, 2021 As the largest chamber of the ruminant stomach, the rumen not only serves as the principal absorptive surface and nutrient transport pathway from the lumen into the animal, but also plays an important short-chain fatty acid (SCFA) metabolic role in ...
Juan Zhao +11 more
doaj +1 more source
Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]
, 2020 The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes +5 more
core +1 more source
Insects, 2021 Bactrocera dorsalis is a major pest that causes serious damage to many fruits. Although phytosanitary treatment methods have been developed for Bactrocera control, there is a lack of information related to the gene expression pattern of B.
Yue Cao +5 more
doaj +1 more source