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Brief guide to RT-qPCR [PDF]

Molecules and Cells
: RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature ...
Dajeong Bong, Jooyeon Sohn, Seung-Jae V. Lee   +2 more
doaj   +4 more sources

Tutorial: Guidelines for Single-Cell RT-qPCR [PDF]

Cells, 2021
Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha, Mikael Kubista, Lukas Valihrach   +2 more
doaj   +3 more sources

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells [PDF]

Biological Procedures Online, 2010
Background Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs
Chen Gang, Kronenberger Peter, Teugels Erik, De Grève Jacques   +3 more
doaj   +2 more sources

A Portable Dual-Mode Microfluidic Device Integrating RT-qPCR and RT-LAMP for Rapid Nucleic Acid Detection in Point-of-Care Testing [PDF]

Biosensors
Point-of-care testing (POCT) has emerged as a vital diagnostic approach in emergency medicine, primary care, and resource-limited environments because of its convenience, affordability, and capacity to provide immediate results.
Baihui Zhang, Xiao Li, Mengjie Huang, Maojie Jiang, Leilei Du, Peng Yin, Xuan Fang, Xiangyu Jiang, Feihu Qi, Yanna Lin, Fuqiang Ma   +10 more
doaj   +2 more sources

Rapid and accurate quantification of isomiRs by RT-qPCR

Scientific Reports, 2022
Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we
Sandra Franco, Raquel Pluvinet, Jose Francisco Sanchez-Herrero, Lauro Sumoy, Miguel Angel Martinez   +4 more
doaj   +5 more sources

TaqMan RT-qPCR for tRNA half quantification. [PDF]

Methods Enzymol
When quantifying tRNA-derived short non-coding RNAs (sncRNAs), two key considerations must be addressed. First, sequencing analyses have revealed significant heterogeneity in the lengths and terminal sequences of tRNA-derived sncRNAs. Second, within the total RNA fraction, these sncRNAs coexist with more abundant mature tRNAs and their precursors (pre ...
Shigematsu M, Kawamura T, Kirino Y.
europepmc   +3 more sources

Intratumoral heterogeneity of microRNA expression in rectal cancer [PDF]

, 2016
Introduction: An increasing number of studies have investigated microRNAs (miRNAs) as potential markers of diagnosis, treatment and prognosis.
A Esquela-Kerscher, AHM Eriksen, Anders Jakobsen, Ane Lindegaard Appelt, Anne Haahr Mellergaard Eriksen, Boye Schnack Nielsen, BS Nielsen, CJ Wang, D Hanahan, D Hanahan, EA Kheirelseid, EM Laurila, Flemming Brandt Sørensen, GH Heppner, HJ Peltier, J Peveling-Oberhag, JG Kuremsky, KH Chang, KN Knudsen, M Agostini, M Raychaudhuri, M Svoboda, O Slaby, O Slaby, PA Davoren, Rajvir Dahiya, Rikke Fredslund Andersen, S Jorgensen, Scarpati G Della Vittoria, SH Lim, T Li, Torben Frøstrup Hansen, U Drebber, W Filipowicz, Z Wotschofsky   +34 more
core   +24 more sources

A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System. [PDF]

PLoS ONE, 2016
Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR).
Coralie Coudray-Meunier, Audrey Fraisse, Sandra Martin-Latil, Sabine Delannoy, Patrick Fach, Sylvie Perelle   +5 more
doaj   +1 more source

Selection of stable reference genes for RT–qPCR in Salmo trutta

Aquaculture Reports, 2022
To identify suitable reference genes for Salmo trutta, real-time fluorescent quantitative PCR (RT–qPCR) technology was used to determine the expression of eight candidate genes in 11 tissues (heart, liver, spleen, head kidney, muscle, brain, gills, ovary,
Shuaijie Sun, Zhitong Wang, Dongdong Yuan, Mengke Ni, Huifen Xu, Wanliang Wang, Chi Zhang, Meiqun Chen, Lamu ZhaXi, Jianshe Zhou, Ming Li   +10 more
doaj   +1 more source

In Situ Capture RT-qPCR Method for Detection of Human Norovirus in Food and Environmental Samples

Proceedings, 2020
Human noroviruses (HuNoVs) are the major cause of non-bacterial acute gastroenteritis worldwide. RT-qPCR is a widely used method to detect HuNoVs. However, the method is unable to extract a virus from environmental samples and to discriminate between ...
Peng Tian
doaj   +1 more source