Results 1 to 10 of about 917,459 (311)
Tutorial: Guidelines for Single-Cell RT-qPCR [PDF]
Cells, 2021 Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha +2 more
doaj +3 more sources
Primer PICKR: literature-mined scoring platform for robust RT–qPCR primers [PDF]
Nature CommunicationsReliable reverse-transcription quantitative PCR (RT–qPCR) depends on well-designed primers, yet undocumented or poorly validated sequences continue to compromise reproducibility. Existing resources catalog only modest sets of empirically verified primers
Thomas G. Molley +4 more
doaj +2 more sources
Molecules and Cells: RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature ...
Dajeong Bong +2 more
doaj +3 more sources
Rapid and accurate quantification of isomiRs by RT-qPCR
Scientific Reports, 2022 Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we
Sandra Franco +4 more
doaj +5 more sources
COVID-19 diagnosis by RT-qPCR in alternative specimens [PDF]
Memorias do Instituto Oswaldo Cruz, 2021 BACKGROUND The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent ...
Cássia Cristina Alves Gonçalves +22 more
doaj +4 more sources
Selection of stable reference genes for RT–qPCR in Salmo trutta
Aquaculture Reports, 2022 To identify suitable reference genes for Salmo trutta, real-time fluorescent quantitative PCR (RT–qPCR) technology was used to determine the expression of eight candidate genes in 11 tissues (heart, liver, spleen, head kidney, muscle, brain, gills, ovary,
Shuaijie Sun +10 more
doaj +1 more source
A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System. [PDF]
PLoS ONE, 2016 Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR).
Coralie Coudray-Meunier +5 more
doaj +1 more source
, 2023 The primer sequences for RT-qPCR targeting each indicated gene. Baf155, Brg1/Brm-associated factor 155; CISH, cytokine inducible SH2 containing protein; F, forward; Glut, glucose transporter; LDHA, lactate dehydrogenase A; MyHC, myosin heavy chain; Pgk ...
Ji-Yun Seo (400939) +13 more
core +2 more sources
Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection
International Journal of Infectious Diseases, 2021 Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study
Masato Inaba +7 more
doaj +1 more source
In Situ Capture RT-qPCR Method for Detection of Human Norovirus in Food and Environmental Samples
Proceedings, 2020 Human noroviruses (HuNoVs) are the major cause of non-bacterial acute gastroenteritis worldwide. RT-qPCR is a widely used method to detect HuNoVs. However, the method is unable to extract a virus from environmental samples and to discriminate between ...
Peng Tian
doaj +1 more source