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Molecules and Cells: RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature ...
Dajeong Bong +2 more
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Tutorial: Guidelines for Single-Cell RT-qPCR [PDF]
Cells, 2021 Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha +2 more
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Rapid and accurate quantification of isomiRs by RT-qPCR
Scientific Reports, 2022 Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we
Sandra Franco +4 more
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COVID-19 diagnosis by RT-qPCR in alternative specimens [PDF]
Memorias do Instituto Oswaldo Cruz, 2021 BACKGROUND The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent ...
Cássia Cristina Alves Gonçalves +22 more
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RT-qPCR primer sequences. [PDF]
, 2023 The primer sequences for RT-qPCR targeting each indicated gene. Baf155, Brg1/Brm-associated factor 155; CISH, cytokine inducible SH2 containing protein; F, forward; Glut, glucose transporter; LDHA, lactate dehydrogenase A; MyHC, myosin heavy chain; Pgk ...
Ji-Yun Seo (400939) +13 more
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RT-qPCR and ChIP-qPCR primers. [PDF]
, 2021 RT-qPCR and ChIP-qPCR primers.
Hilary T. Brewis (11798006) +5 more
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Selection of stable reference genes for RT–qPCR in Salmo trutta
Aquaculture Reports, 2022 To identify suitable reference genes for Salmo trutta, real-time fluorescent quantitative PCR (RT–qPCR) technology was used to determine the expression of eight candidate genes in 11 tissues (heart, liver, spleen, head kidney, muscle, brain, gills, ovary,
Shuaijie Sun +10 more
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A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System. [PDF]
PLoS ONE, 2016 Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR).
Coralie Coudray-Meunier +5 more
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RT-qPCR-based confirmation of RNA-sequencing results using both LNA GapmeRs against TUG1. [PDF]
, 2022 HUVECs were transfected with two LNA GapmeRs against TUG1—LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl and expression levels of (A) VAMP4, (B) TOR1AIP2, (C) KAT6B and (D) ABCA1 were measured after 48 hours by RT-qPCR. Expression is relative to P0 (n = 4; SEM;
Tamer Ali (632879) +14 more
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