Results 41 to 50 of about 795,270 (311)
Brazilian Journal of Infectious Diseases, 2023 Introdução/objetivos: As gastroenterites agudas (GEA) são doenças que se constituem como a segunda maior causa de morte em crianças de todo mundo, especialmente em crianças menores de cinco anos, ocorrendo principalmente em países de baixa e média renda.
Ana Luiza da Mota Raminho +5 more
doaj +1 more source
International Journal of Statistics in Medical Research, 2012 We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.
Perkins, James R. +2 more
openaire +2 more sources
RT-qPCR Testing of SARS-CoV-2: A Primer [PDF]
International Journal of Molecular Sciences, 2020 Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR)
Stephen A. Bustin, Tania Nolan
openaire +3 more sources
Validation of extracellular miRNA quantification in blood samples using RT‐qPCR
FASEB BioAdvances, 2019 Extracellular microRNAs (miRs) have been proposed as important blood‐based biomarkers for several diseases. Contrary to proteins and other RNA classes, miRs are stable and easily detectable in body fluids.
Maria Fauth +4 more
doaj +1 more source
RT-qPCR expression of chosen genes from RNA-Seq. [PDF]
, 2022 Testis expression of 15 genes measured with RNA-Seq and RT-qPCR of dominant fish compared to subordinate fish. Values are expressed as log2fold change +/- SEM. Significant differences for RT-qPCR data was determined via ANOVA. * = p< 0.05.
Berta Levavi-Sivan (2456017) +3 more
core +1 more source
Journal of Integrative Agriculture, 2014 Two microRNA (miRNA) quantification methods, namely, poly(A) reverse transcription (RT)-quantitative real-time polymerase chain reaction (qPCR) and stem-loop RT-qPCR, have been developed for quantifying miRNA expression. In the present study, five miRNAs,
Ran HAN +3 more
doaj +1 more source