Results 61 to 70 of about 495,188 (306)

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR

Journal of Clinical Virology Plus
Background: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward.
David Alejandro Cabrera Gaytán, Porfirio Felipe Hernández Bautista, Clara Esperanza Santacruz Tinoco, Julio Elias Alvarado Yaah, Yu-Mei Anguiano Hernández, Bernardo Martínez Miguel, Alfonso Vallejos Parás, Lumumba Arriaga Nieto, Nancy Sandoval Gutiérrez, Concepción Grajales Muñiz, Alejandro Moctezuma Paz, Leticia Jaimes Betancourt   +11 more
doaj   +1 more source

Detection of MicroRNAs in Dried Serum Blots [PDF]

, 2010
MicroRNAs are short RNAs which can be utilized as biomarkers for a variety of conditions. They are detectable in serum, and changes in the levels of circulating microRNAs have been associated with different diseases.
Reema Mallick, Sai Yendamuri, Santosh K. Patnaik   +2 more
core   +2 more sources

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Efficacy of bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR for detecting neonatal sepsis: a case control study

BMC Pediatrics, 2010
Background Neonatal sepsis is difficult to diagnose and pathogens cannot be detected from blood cultures in many cases. Development of a rapid and accurate method for detecting pathogens is thus essential.
Sato Hiroaki, Shoji Hiromichi, Komatsu Mitsutaka, Matsunaga Nobuaki, Nagata Satoru, Hisata Ken, Fujimori Makoto, Yamashiro Yuichiro, Asahara Takashi, Nomoto Koji, Shimizu Toshiaki   +10 more
doaj   +1 more source

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR. [PDF]

PLoS ONE, 2016
Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to ...
Lele Song, Yuemin Li, Jia Jia, Guangpeng Zhou, Jianming Wang, Qian Kang, Peng Jin, Jianqiu Sheng, Guoxiang Cai, Sanjun Cai, Xiaoliang Han   +10 more
doaj   +1 more source

Biological subtyping of early breast cancer : a study comparing RT-qPCR with immunohistochemistry [PDF]

, 2016
The biological subtype of breast cancer influences the selection of systemic therapy. Distinction between luminal A and B cancers depends on consistent assessment of Ki-67, but substantial intra-observer and inter-observer variability exists when ...
Auvinen, Paivi, Eidt, Sebastian, Heikkila, Paivi, Isola, Jorma, Joensuu, Heikki, Jyrkkio, Sirkku, Kellokumpu-Lehtinen, Pirkko-Liisa, Laible, Mark, Lakis, Sotiris, Sahin, Ugur, Schlombs, Kornelia, Sihto, Harri, Turpeenniemi-Hujanen, Taina, Weber, Stefan, Wirtz, Ralph M.   +14 more
core   +2 more sources

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing

Plants, 2022
C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C.
Luis Rubio, Francisco J. J. Guinot-Moreno, Carmen Sanz-López, Luis Galipienso   +3 more
doaj   +1 more source

Innate immune system activation in zebrafish and cellular models of Diamond Blackfan Anemia. [PDF]

, 2018
Deficiency of ribosomal proteins (RPs) leads to Diamond Blackfan Anemia (DBA) associated with anemia, congenital defects, and cancer. While p53 activation is responsible for many features of DBA, the role of immune system is less defined.
Bibikova, Elena, Danilova, Nadia, Lin, Shuo, Sakamoto, Kathleen M, Wilkes, Mark, Youn, Min-Young   +5 more
core   +2 more sources