Results 61 to 70 of about 795,270 (311)
Primer PICKR: literature-mined scoring platform for robust RT–qPCR primers
Nature CommunicationsReliable reverse-transcription quantitative PCR (RT–qPCR) depends on well-designed primers, yet undocumented or poorly validated sequences continue to compromise reproducibility. Existing resources catalog only modest sets of empirically verified primers
Thomas G. Molley +4 more
doaj +1 more source
Custom RT-qPCR-array for glaucoma filtering surgery prognosis. [PDF]
PLoS ONE, 2017 Excessive subconjunctival scarring is the main reason of failure of glaucoma filtration surgery. We analyzed conjunctival and systemic gene expression patterns after non penetrating deep sclerectomy (NPDS). To find expression patterns related to surgical
Iñaki Rodriguez-Agirretxe +4 more
doaj +1 more source
Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province [PDF]
Iraqi Journal of Veterinary Sciences, 2020 Avian reoviruses can infect birds without any clinical signs of infection, the infection may associate with different manifestations including viral arthritis/tenosynovitis and malabsorption syndrome.
Fanar Isihak
doaj +1 more source
Digestive enzymes RT-qPCR [PDF]
, 2017 The raw data (expressed as Ct-values) from RT-qPCR on genes for digestive proteases and amylases and maltases, measured in larvae of Control and Selected populations, developing at the poor diet at two stages, (early and late 3rd instar).
van der Meer, Jan R. +7 more
core +1 more source
RT-qPCR primer sequences used in the present study. [PDF]
, 2023 RT-qPCR primer sequences used in the present study.
Joong-Woon Lee (15332874) +8 more
core +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Plant Methods, 2022 Background Seeds were an important medium for long-distance transmission of plant viruses. Therefore, appropriate, more sensitive methods for detecting low concentrations of virus-infected in seeds were crucial to ensure the quality of seed lots. In this
Yin Xinying +5 more
doaj +1 more source
Molecular Oncology, EarlyView.Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano +6 more
wiley +1 more source
High-throughput microfluidic single-cell RT-qPCR [PDF]
Proceedings of the National Academy of Sciences, 2011 A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run.
Adam K, White +8 more
openaire +2 more sources
RT-qPCR assay (example of a real case). [PDF]
, 2022 RT-qPCR assay (example of a real case).
Antonella Pacini (14059507) +9 more
core +1 more source