Results 51 to 60 of about 917,459 (311)

Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR

Advanced Science, 2023
The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic
Kiran Shrestha, Seongryeong Kim, Jiyeon Han, Gabriela Morales Florez, Han Truong, Trung Hoang, Sajjan Parajuli, Tiara AM, Beomsoo Kim, Younsu Jung, Abdurhaman Teyib Abafogi, Yugyeong Lee, Seung Hyun Song, Jinkee Lee, Sungsu Park, Minhee Kang, Hee Jae Huh, Gyoujin Cho, Luke P. Lee   +18 more
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

RT-qPCR Primer Pairs.

, 2016
RT-qPCR Primer Pairs.
Vittoria Roncalli (524400), Michelle J. Jungbluth (3153261), Petra H. Lenz (524399)   +2 more
core   +1 more source

RT-qPCR and qPCR assay details and performance for the viral targets.

, 2023
RT-qPCR and qPCR assay details and performance for the viral targets.
Kata Farkas (3174156), Jasmine M. S. Grimsley (14475076), Natasha Alex-Sanders (14475073), Nick Woodhall (14475082), Igor Pântea (14475079), Davey L. Jones (7368803), Matthew J. Wade (3821401), Rachel Williams (372509)   +7 more
core   +1 more source

Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR

Plant Methods, 2022
Background Seeds were an important medium for long-distance transmission of plant viruses. Therefore, appropriate, more sensitive methods for detecting low concentrations of virus-infected in seeds were crucial to ensure the quality of seed lots. In this
Yin Xinying, Li Xin, Yang Lili, Zheng Qiuyue, Piao Yongzhe, Cao Jijuan   +5 more
doaj   +1 more source

Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing

Plants, 2022
C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C.
Luis Rubio, Francisco J. J. Guinot-Moreno, Carmen Sanz-López, Luis Galipienso   +3 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

RT-qPCR validation.

, 2021
Scatter plot shows gene expression fold changes calculated from RT-qPCR data and RNA-seq data. The correlation coefficient (R2) value is = 0.98. The fold changes are from the comparison between the R-treated and the W-treated C.
Theerayut Toojinda (4018604), Atchara Paemanee (171376), Wanapinun Nawae (669298), Sithichoke Tangphatsornruang (399948), Nattapol Narong (11767446), Wirulda Pootakham (680893), Yanisa Ketngamkum (11767449), Kanokwan Romyanon (11767452), Thippawan Yoocha (680898)   +8 more
core   +1 more source

Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR

Journal of Clinical Virology Plus
Background: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward.
David Alejandro Cabrera Gaytán, Porfirio Felipe Hernández Bautista, Clara Esperanza Santacruz Tinoco, Julio Elias Alvarado Yaah, Yu-Mei Anguiano Hernández, Bernardo Martínez Miguel, Alfonso Vallejos Parás, Lumumba Arriaga Nieto, Nancy Sandoval Gutiérrez, Concepción Grajales Muñiz, Alejandro Moctezuma Paz, Leticia Jaimes Betancourt   +11 more
doaj   +1 more source

VLP RT-qPCR v1

, 2017
VLP RT-qPCR
Motoko Tanaka, Brook C Barajas, Jaisri Lingappa   +2 more
openaire   +1 more source