Results 71 to 80 of about 952,520 (310)

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR

Journal of Clinical Virology Plus
Background: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward.
David Alejandro Cabrera Gaytán, Porfirio Felipe Hernández Bautista, Clara Esperanza Santacruz Tinoco, Julio Elias Alvarado Yaah, Yu-Mei Anguiano Hernández, Bernardo Martínez Miguel, Alfonso Vallejos Parás, Lumumba Arriaga Nieto, Nancy Sandoval Gutiérrez, Concepción Grajales Muñiz, Alejandro Moctezuma Paz, Leticia Jaimes Betancourt   +11 more
doaj   +1 more source

RT-qPCR T1RsT2Rs_English

, 2013
RT-qPCR ...
Saito, Shouichiro, Sae Gonda (3265965), Shuichi Matsumura (53739), Hiroo Imai (135922), Yasuhiro Go (143414), Shouichiro Saito (485034), Go, Yasuhiro, Imai, Hiroo, Gonda, Sae, Matsumura, Shuichi   +9 more
core   +1 more source

Inhibition of cyclin‐dependent kinases 12/13 using CT7439 as a treatment for colorectal cancer with CDK12 upregulation

Molecular Oncology, EarlyView.
The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington, Divya L. Dayanidhi, Mohammad Zokaasadi, John B. Mantyh, Pelumi D. Olawuni, Gabrielle Rupprecht, Jeremy M. Force, Shannon McCall, Ashwani K. Bahl, David S. Hsu, Jason A. Somarelli   +10 more
wiley   +1 more source

Efficacy of bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR for detecting neonatal sepsis: a case control study

BMC Pediatrics, 2010
Background Neonatal sepsis is difficult to diagnose and pathogens cannot be detected from blood cultures in many cases. Development of a rapid and accurate method for detecting pathogens is thus essential.
Sato Hiroaki, Shoji Hiromichi, Komatsu Mitsutaka, Matsunaga Nobuaki, Nagata Satoru, Hisata Ken, Fujimori Makoto, Yamashiro Yuichiro, Asahara Takashi, Nomoto Koji, Shimizu Toshiaki   +10 more
doaj   +1 more source

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR. [PDF]

PLoS ONE, 2016
Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to ...
Lele Song, Yuemin Li, Jia Jia, Guangpeng Zhou, Jianming Wang, Qian Kang, Peng Jin, Jianqiu Sheng, Guoxiang Cai, Sanjun Cai, Xiaoliang Han   +10 more
doaj   +1 more source

RT-qPCR gustducin_English

, 2013
RT-qPCR ...
Saito, Shouichiro, Sae Gonda (3265965), Shuichi Matsumura (53739), Hiroo Imai (135922), Yasuhiro Go (143414), Shouichiro Saito (485034), Go, Yasuhiro, Imai, Hiroo, Gonda, Sae, Matsumura, Shuichi   +9 more
core   +1 more source

High-throughput microfluidic single-cell RT-qPCR [PDF]

Proceedings of the National Academy of Sciences, 2011
A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run.
Adam K, White, Michael, VanInsberghe, Oleh I, Petriv, Mani, Hamidi, Darek, Sikorski, Marco A, Marra, James, Piret, Samuel, Aparicio, Carl L, Hansen   +8 more
openaire   +2 more sources

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

Biological Procedures Online, 2010
Background Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs
Chen Gang, Kronenberger Peter, Teugels Erik, De Grève Jacques   +3 more
doaj   +1 more source