Results 71 to 80 of about 795,270 (311)

EDNRB‐dependent endothelin signaling reduces proliferation and promotes proneural‐to‐mesenchymal transition in gliomas

Molecular Oncology, EarlyView.
Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau, Leonor Garcia, Hugo Arnold, Antonija Hanžek, Maialen Arrieta, Laurent R Gauthier, Christine Granotier‐Beckers, François D Boussin, Amaury Herbet, Marie Hautière, Valentin Asei‐Ceschino, Clémentin Jacques, Laura Brard, Thomas Harnois, Valérie Coronas, Bruno Constantin, Aurélien Chatelier, Jean Chemin, Serge Urbach, Martial Seveno, Szimonetta Hideg, Chantal Ripoll, Kasandra Aguilar‐Cázarez, Min Zheng, Guo‐Hao Huang, Sheng‐Qing Lv, Lei Zhang, Philippe Rondard, Laurent Prezeau, Jean‐Philippe Pin, Hugues Duffau, Luc Bauchet, Valérie Rigau, Franck Denat, Charles Truillet, Didier Boquet, Jean‐Philippe Hugnot   +36 more
wiley   +1 more source

Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR

Journal of Clinical Virology Plus
Background: Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward.
David Alejandro Cabrera Gaytán, Porfirio Felipe Hernández Bautista, Clara Esperanza Santacruz Tinoco, Julio Elias Alvarado Yaah, Yu-Mei Anguiano Hernández, Bernardo Martínez Miguel, Alfonso Vallejos Parás, Lumumba Arriaga Nieto, Nancy Sandoval Gutiérrez, Concepción Grajales Muñiz, Alejandro Moctezuma Paz, Leticia Jaimes Betancourt   +11 more
doaj   +1 more source

Discovery and Diagnosis of a New Sobemovirus Infecting Cyperus esculentus Showing Leaf Yellow Mosaic and Dwarfism Using Small-RNA High Throughput Sequencing

Plants, 2022
C. esculentus is a profitable crop in Valencia, Spain, but the emergence of a disease causing of leaf yellow mosaic, dwarfism, and a drastic decrease in tuber production has become a problem. The small-RNA high-throughput sequencing (HTS) of a diseased C.
Luis Rubio, Francisco J. J. Guinot-Moreno, Carmen Sanz-López, Luis Galipienso   +3 more
doaj   +1 more source

RT-qPCR. [PDF]

, 2019
mRNA expression of induced-pluripotent stem cell markers OCT4 (A), SOX2 (B), NANOG (C), KLF4 (D) and c-MYC (E) detected by RT-qPCR. Data displayed as the fold-change of gene expression in tumor samples relative to their patient-matched normal colon ...
Swee T. Tan (5120171), Tinte Itinteang (5120168), Susrutha K. Wickremesekera (5645231), Matthew J. Munro (7354253), Lifeng Peng (709111), Reginald W. Marsh (5645237)   +5 more
core   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR. [PDF]

PLoS ONE, 2016
Epigenetic markers based on differential methylation of DNA sequences are used in cancer screening and diagnostics. Detection of abnormal methylation at specific loci by real-time quantitative polymerase chain reaction (RT-qPCR) has been developed to ...
Lele Song, Yuemin Li, Jia Jia, Guangpeng Zhou, Jianming Wang, Qian Kang, Peng Jin, Jianqiu Sheng, Guoxiang Cai, Sanjun Cai, Xiaoliang Han   +10 more
doaj   +1 more source

RT-qPCR validation of CHRNA5 RNAi microarray data. [PDF]

, 2018
A. RT-qPCR validation of selected genes modulated by siRNA-1 in MCF7 cells. B. RT-qPCR validation of selected genes in siRNA-2 and siRNA-3 treated MCF7 cells. C.
Ozlen Konu (412799), Sahika Cingir Koker (6082748), Huma Shehwana (6082751), Ermira Jahja (1753669), Ayse Gokce Keskus (6082754)   +4 more
core   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Comparing the Effectiveness of Colorimetric Reverse Transcription-Loop-Mediated Isothermal Amplification (SCoV-2 Detection Kit L1) with that of Reverse Transcription-Quantitative Polymerase Chain Reaction in SARS-CoV-2 Detection

Journal of Health Science and Medical Research (JHSMR)
Objective: To determine the diagnostic sensitivity and specificity of reverse transcription-loop-mediated isothermal amplification (RT-LAMP) compared to those of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for coronavirus ...
Aekkaraj Nualla-ong, Rongrit Oplod, Phattharaphon Rattanaareeyakorn, Sommanpat Surasombatpattana, Pisud Siripaitoon, Narongdet Kositpantawong, Siripen Kanchanasuwan, Sorawit Chittrakarn, Boonsri Charoenmak, Monchana Jullangkoon, Arnon Chukamnerd, Sarunyou Chusri   +11 more
doaj   +1 more source

Efficacy of bacterial ribosomal RNA-targeted reverse transcription-quantitative PCR for detecting neonatal sepsis: a case control study

BMC Pediatrics, 2010
Background Neonatal sepsis is difficult to diagnose and pathogens cannot be detected from blood cultures in many cases. Development of a rapid and accurate method for detecting pathogens is thus essential.
Sato Hiroaki, Shoji Hiromichi, Komatsu Mitsutaka, Matsunaga Nobuaki, Nagata Satoru, Hisata Ken, Fujimori Makoto, Yamashiro Yuichiro, Asahara Takashi, Nomoto Koji, Shimizu Toshiaki   +10 more
doaj   +1 more source