Results 81 to 90 of about 495,188 (306)

Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

The EuroBiotech Journal, 2021
A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2.
Tuncel Gulten, Ergoren Mahmut Cerkez, Baddal Buket, Tulay Pinar, Arikan Ayse, Guler Emrah, Ozverel Cenk Serhan, Kaya Suer H., Sayan Murat, Sanlidag Tamer,   +10 more
doaj   +1 more source

The Long Noncoding RNA HEAL Regulates HIV-1 Replication through Epigenetic Regulation of the HIV-1 Promoter. [PDF]

, 2019
A major challenge in finding a cure for HIV-1/AIDS is the difficulty in identifying and eradicating persistent reservoirs of replication-competent provirus.
Chang, Kungyen, Chao, Ti-Chun, Karris, Maile Ann Young, Kaul, Marcus, Li, Zhonghan, Qin, Yue, Rana, Tariq M, Sanchez, Ana, Singh, Gatikrushna, Tiwari, Shashi Kant, Yau, Edwin, Zhang, Qiong   +11 more
core   +1 more source

RT-qPCR v1

, 2017

openaire   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR.

PLoS ONE
IntroductionDengue fever is a growing global concern with an estimated 100-400 million infections every year and rising mortality over the past decade. In 2017, 40,000 deaths were attributed to dengue. Real-time reverse transcription PCR (RT-qPCR) is the
Vilayouth Phimolsannousith, Malavanh Vongsouvath, Padthana Kiedsathid, Manivanh Vongsouvath, Elizabeth A Ashley, Audrey Dubot-Pérès   +5 more
doaj   +1 more source

Simultaneous detection of human norovirus GI, GII and SARS-CoV-2 by a quantitative one-step triplex RT-qPCR

Frontiers in Microbiology
BackgroundThere are many similarities in the clinical manifestations of human norovirus and SARS-CoV-2 infections, and nucleic acid detection is the gold standard for diagnosing both diseases. In order to expedite the identification of norovirus and SARS-
Hua-Wei Yi, Hua-Wei Yi, Xian-Mo Wang, Xian-Mo Wang, Xin Tan, Cai-Zhi Ding, Chang-Li Zhang, Chang-Li Zhang, Jia-Hao Wu, Jia-Hao Wu, Qi Li, Qi Li, Chen-Qi Xin, Chen-Qi Xin, Wen Fan, Wen Fan   +15 more
doaj   +1 more source

RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings

Bio-Protocol, 2020
Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions.
Tianran Jia, Brandon Le
doaj   +1 more source

Development and characterization of a new human hepatic cell line [PDF]

, 2015
The increasing demand and hampered use of primary human hepatocytes for research purposes have urged scientists to search for alternative cell sources, such as immortalized hepatic cell lines.
Berx, Geert, De Craene, Bram, De Kock, Joey, Ramboer, Eva, Rogiers, Vera, Vanhaecke, Tamara, Vinken, Mathieu   +6 more
core   +3 more sources

RT-qPCR v1

Gene expression levels by RT-qPCR
Karyna Tarasova, Sinan Gültekin, Angkana Kidtiwong, iris.gerner Gerner, Florien Jenner   +4 more
openaire   +1 more source

RNA Extraction and RT-qPCR v1

, 2022
This protocol is used to extract RNA from mouse brain tissue and quantify with RT-qPCR.
openaire   +1 more source