Results 81 to 90 of about 795,270 (311)

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

Biological Procedures Online, 2010
Background Real-time quantitative RT-PCR (RT-qPCR) is a "gold" standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs
Chen Gang, Kronenberger Peter, Teugels Erik, De Grève Jacques   +3 more
doaj   +1 more source

Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR

Advanced Science, 2023
The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic
Kiran Shrestha, Seongryeong Kim, Jiyeon Han, Gabriela Morales Florez, Han Truong, Trung Hoang, Sajjan Parajuli, Tiara AM, Beomsoo Kim, Younsu Jung, Abdurhaman Teyib Abafogi, Yugyeong Lee, Seung Hyun Song, Jinkee Lee, Sungsu Park, Minhee Kang, Hee Jae Huh, Gyoujin Cho, Luke P. Lee   +18 more
doaj   +1 more source

RT-qPCR data and aligned RIP sequences [PDF]

, 2017
RT-qPCR data and aligned RIP ...
Steve J. Perlman (8037953), Ballinger, Matthew J., Matthew J. Ballinger (3319482), Perlman, Steve J.   +3 more
core   +1 more source

RT-qPCR standard curve using Twist RNA. [PDF]

, 2022
Different copy numbers of Twist RNA (5, 10, 100, 1000, and 10000) were used to generate a standard curve using the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit. Amplification and standard curves from both N1 and N2 targets are shown. (TIF)
Prem K. Premsrirut (12646751), Jacqueline L. Bruce (12133417), Bradley W. Langhorst (8725818), Nathan A. Tanner (9400708), Catherine B. Poole (9455853), Jacob Miller (6099845), Guoping Ren (112954), Zhiru Li (295010), William E. Jack (12646742), Clotilde K. S. Carlow (9455856), Reynes A. Moncion (12646748), Richard J. Roberts (8946), Yinhua Zhang (111234), Katell Kunin (12646745), Barry Cohen (12646736), Caileigh V. Cunningham (12646739)   +15 more
core   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Evaluation of the SMARTCHEK Genesystem RT-qPCR assay for the detection of SARS-CoV-2 in clinical samples

BMC Infectious Diseases, 2022
Background The COVID-19 pandemic remains the main public health problem, due to the quick and easy dissemination of the causal agent, SARS-CoV-2 virus, around the world.
Diana Flores-León, Willi Quino, Alejandra E. Cúneo, Junior Caro-Castro, Evans Cucho-Meza, Ronnie G. Gavilan   +5 more
doaj   +1 more source

RT-qPCR v1

Gene expression levels by RT-qPCR
Karyna Tarasova, Sinan Gültekin, Angkana Kidtiwong, iris.gerner Gerner, Florien Jenner   +4 more
openaire   +1 more source

RT-qPCR vs CRISPR in SARS-CoV-2. [PDF]

, 2022
RT-qPCR vs CRISPR in SARS-CoV-2.
Xiao Ge (3909412), Xiaoyu Zhang (62901), Yubo Zhang (224930), Fangyuan Shen (630124), Jinjuan Qiao (14014470), Heng Li (6476)   +5 more
core   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source