Results 91 to 100 of about 917,459 (311)

RNA Extraction and RT-qPCR v1

, 2022
This protocol is used to extract RNA from mouse brain tissue and quantify with RT-qPCR.
openaire   +1 more source

Gut microbiota alterations in patients with non‐small‐cell lung cancer undergoing chemoradiotherapy

Molecular Oncology, EarlyView.
We investigated the impact of concurrent chemoradiotherapy (CRT) on the gut microbiota in patients with locally advanced non‐small‐cell lung cancer. Overall gut microbiota composition remained largely stable throughout CRT while antibiotic exposure may influence microbial diversity.
Hanne Marte Nymoen, Zhi Zhao, Henrik Horndalsveen, Maria Moksnes Bjaanæs, Bjørn Henning Grønberg, Tarje Halvorsen, Tesfaye Madebo, Marianne Aanerud, Jussi Koivunen, Kersti Oselin, Saulius Cicenas, Nina Helbekkmo, Jarkko Ahvonen, Maria Silvoniemi, Tine Norman Alver, Tonje Dalen, Åsa Kristina Öjlert, Saima Jamil Farooqi, Åslaug Helland, Vilde Drageset Haakensen   +19 more
wiley   +1 more source

RT-qPCR T1RsT2Rs_English

, 2013
RT-qPCR ...
Saito, Shouichiro, Sae Gonda (3265965), Shuichi Matsumura (53739), Hiroo Imai (135922), Yasuhiro Go (143414), Shouichiro Saito (485034), Go, Yasuhiro, Imai, Hiroo, Gonda, Sae, Matsumura, Shuichi   +9 more
core   +1 more source

Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study

JMIR Public Health and Surveillance
BackgroundMany countries have applied the wastewater surveillance of the COVID-19 pandemic to their national public health monitoring measures.
Annika Länsivaara, Kirsi-Maarit Lehto, Rafiqul Hyder, Erja Sinikka Janhonen, Anssi Lipponen, Annamari Heikinheimo, Tarja Pitkänen, Sami Oikarinen   +7 more
doaj   +1 more source

Canine distemper virus detection by different methods of One-Step RT-qPCR

Ciência Rural, 2016
: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical ...
Claudia de Camargo Tozato, Vívian Ferreira Zadra, Caroline Rodrigues Basso, João Pessoa Araújo Junior   +3 more
doaj   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

RT-qPCR TRPM5_English

, 2013
RT-qPCR ...
Saito, Shouichiro, Sae Gonda (3265965), Shuichi Matsumura (53739), Hiroo Imai (135922), Yasuhiro Go (143414), Shouichiro Saito (485034), Go, Yasuhiro, Imai, Hiroo, Gonda, Sae, Matsumura, Shuichi   +9 more
core   +1 more source

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]

, 2012
Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Wagner, Carola, Pfaffl, Michael W., Meyer, Swanhild U., Kaiser, Sebastian, Thirion, Christian   +4 more
core   +1 more source

Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms

Frontiers in Genetics, 2018
MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3′-end length variants
Charlotte Nejad, Charlotte Nejad, Geneviève Pépin, Geneviève Pépin, Mark A. Behlke, Michael P. Gantier, Michael P. Gantier   +6 more
doaj   +1 more source

Human GAPDH RT-qPCR v1 [PDF]

, 2018
RNA from matched FFPE, PFPE and cryo preserved human tissues, stored for up to seven years at 22°C and 4°C, examined for integrity and usability in quantitative RT-PCR
openaire   +1 more source