Results 91 to 100 of about 495,188 (306)
CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh +12 more
wiley +1 more source
BackgroundMany countries have applied the wastewater surveillance of the COVID-19 pandemic to their national public health monitoring measures.
Annika Länsivaara +7 more
doaj +1 more source
Canine distemper virus detection by different methods of One-Step RT-qPCR
: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical ...
Claudia de Camargo Tozato +3 more
doaj +1 more source
Post-weaning blood transcriptomic differences between Yorkshire pigs divergently selected for residual feed intake [PDF]
Meta data for RNA-seq and RT-qPCR assays.
Dekkers, Jack +5 more
core +13 more sources
Linear-hairpin variable primer RT-qPCR for MicroRNA
Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR.
Lin Lan +6 more
openaire +3 more sources
Improving the standardization of mRNA measurement by RT-qPCR [PDF]
Human health and safety depend on reliable measurements in medical diagnosis and on tests that support the selection and evaluation of therapeutic intervention and newly discovered molecular biomarkers must pass a rigorous evaluation process if they are to be of benefit to patients.
Rebecca Sanders +3 more
openaire +3 more sources
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau +8 more
wiley +1 more source
Heterogeneous expression of EPCAM in human circulating tumour cells from patient-derived xenografts
Background We aim to characterize the heterogeneous circulating tumour cells (CTCs) in peripheral blood, independently of physical or immunological purification, by using patient-derived xenografts (PDXs) models.
Chiara Agnoletto +9 more
doaj +1 more source
Evaluation of eleven reference genes for Reverse Transcriptase Quantitative PCR of rubber tree under water deficit [PDF]
Reverse Transcriptase Quantitative PCR (RTqPCR) is a powerful technique in order to detect low abundance of mRNA in the plant cell. The measurement of transcript abundance is relative to the control of expression such as housekeeping genes.
Leclercq, Julie +2 more
core
Impact of antibiotics on the proliferation and differentiation of human adipose-derived mesenchymal stem cells [PDF]
Adipose tissue is a promising source of mesenchymal stem cells. Their potential to differentiate and regenerate other types of tissues may be affected by several factors. This may be due to in vitro cell-culture conditions, especially the supplementation
Gola, Joanna +6 more
core +4 more sources

