Results 91 to 100 of about 952,520 (310)

Gut microbiota alterations in patients with non‐small‐cell lung cancer undergoing chemoradiotherapy

Molecular Oncology, EarlyView.
We investigated the impact of concurrent chemoradiotherapy (CRT) on the gut microbiota in patients with locally advanced non‐small‐cell lung cancer. Overall gut microbiota composition remained largely stable throughout CRT while antibiotic exposure may influence microbial diversity.
Hanne Marte Nymoen, Zhi Zhao, Henrik Horndalsveen, Maria Moksnes Bjaanæs, Bjørn Henning Grønberg, Tarje Halvorsen, Tesfaye Madebo, Marianne Aanerud, Jussi Koivunen, Kersti Oselin, Saulius Cicenas, Nina Helbekkmo, Jarkko Ahvonen, Maria Silvoniemi, Tine Norman Alver, Tonje Dalen, Åsa Kristina Öjlert, Saima Jamil Farooqi, Åslaug Helland, Vilde Drageset Haakensen   +19 more
wiley   +1 more source

Analysis of ZAP70 expression in adult acute lymphoblastic leukaemia by real time quantitative PCR

Molecular Cytogenetics, 2012
Background ZAP70 gene expression is associated with poor prognosis in B-cell lymphoproliferative disorders especially chronic lymphocytic leukaemia (CLL) but its role in adult B-ALL has not been established.
Chakupurakal Geothy, Bell Andrew, Griffiths Mike, Wandroo Farooq, Moss Paul   +4 more
doaj   +1 more source

Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

The EuroBiotech Journal, 2021
A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2.
Tuncel Gulten, Ergoren Mahmut Cerkez, Baddal Buket, Tulay Pinar, Arikan Ayse, Guler Emrah, Ozverel Cenk Serhan, Kaya Suer H., Sayan Murat, Sanlidag Tamer,   +10 more
doaj   +1 more source

[Considerations for normalisation of RT-qPCR in oncology].

Annales de biologie clinique, 2008
Gene expression analysis has many applications in the management of cancer, including diagnosis, prognosis, and therapeutic care. In this context, the reverse transcription quantitative polymerase chain reaction (RT-qPCR) has become the "gold standard" for mRNA quantification.
Ho-Pun-Cheung, Alexandre, Cellier, Dominic, Lopez-Crapez, Evelyne   +2 more
openaire   +2 more sources

A role for eIF5A in translational control of stress‐inducible proteins in multiple myeloma cell lines

Molecular Oncology, EarlyView.
Loss of eIF5A rewires thapsigargin‐mediated endoplasmic reticulum stress responses in multiple myeloma cells by selective translation of mRNA transcripts, consequently dampening thapsigargin‐induced unfolded protein response (UPR) activation and shifting the balance from induction of apoptosis toward cell survival and proliferation.
Demi Both, Ingrid A. M. Derks, Raphaël Vanleyssem, Marine Leclercq, Najla El Hachem, Pierre Close, Eric Eldering, Marco Vincent Haselager   +7 more
wiley   +1 more source

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR.

PLoS ONE
IntroductionDengue fever is a growing global concern with an estimated 100-400 million infections every year and rising mortality over the past decade. In 2017, 40,000 deaths were attributed to dengue. Real-time reverse transcription PCR (RT-qPCR) is the
Vilayouth Phimolsannousith, Malavanh Vongsouvath, Padthana Kiedsathid, Manivanh Vongsouvath, Elizabeth A Ashley, Audrey Dubot-Pérès   +5 more
doaj   +1 more source

Simultaneous detection of human norovirus GI, GII and SARS-CoV-2 by a quantitative one-step triplex RT-qPCR

Frontiers in Microbiology
BackgroundThere are many similarities in the clinical manifestations of human norovirus and SARS-CoV-2 infections, and nucleic acid detection is the gold standard for diagnosing both diseases. In order to expedite the identification of norovirus and SARS-
Hua-Wei Yi, Hua-Wei Yi, Xian-Mo Wang, Xian-Mo Wang, Xin Tan, Cai-Zhi Ding, Chang-Li Zhang, Chang-Li Zhang, Jia-Hao Wu, Jia-Hao Wu, Qi Li, Qi Li, Chen-Qi Xin, Chen-Qi Xin, Wen Fan, Wen Fan   +15 more
doaj   +1 more source

RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings

Bio-Protocol, 2020
Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions.
Tianran Jia, Brandon Le
doaj   +1 more source

RT-qPCR vs CRISPR in SARS-CoV-2.

, 2022
RT-qPCR vs CRISPR in SARS-CoV-2.
Xiao Ge (3909412), Xiaoyu Zhang (62901), Yubo Zhang (224930), Fangyuan Shen (630124), Jinjuan Qiao (14014470), Heng Li (6476)   +5 more
core   +1 more source

RNA Extraction and RT-qPCR v1

, 2022
This protocol is used to extract RNA from mouse brain tissue and quantify with RT-qPCR.
openaire   +1 more source