Results 91 to 100 of about 795,270 (311)
RT-qPCR validation of DEGs and DEPs. [PDF]
, 2019 A shown the informations of the 16 candidated genes. B shown the result of the RT-qPCR validation of the candidated genes.
Aiying Lei (3514988) +7 more
core +1 more source
MITF maintains genome stability in nonmelanocyte lineages
Molecular Oncology, EarlyView.MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir +13 more
wiley +1 more source
Heterogeneous expression of EPCAM in human circulating tumour cells from patient-derived xenografts
Biomarker Research, 2018 Background We aim to characterize the heterogeneous circulating tumour cells (CTCs) in peripheral blood, independently of physical or immunological purification, by using patient-derived xenografts (PDXs) models.
Chiara Agnoletto +9 more
doaj +1 more source
Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization
Molecular Oncology, EarlyView.In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu +13 more
wiley +1 more source
RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings
Bio-Protocol, 2020 Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions.
Tianran Jia, Brandon Le
doaj +1 more source
RT-qPCR analysis of eel brain samples. [PDF]
, 2019 (A) RT-qPCR analysis of follicle stimulating hormone (fsh), luteinizing hormone (lh), and growth hormone (gh) in cultured and wild female eel brain samples.
Miyuki Mekuchi (2503714) +3 more
core +1 more source
Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer
Molecular Oncology, EarlyView.Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola +11 more
wiley +1 more source
Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR.
PLoS ONEIntroductionDengue fever is a growing global concern with an estimated 100-400 million infections every year and rising mortality over the past decade. In 2017, 40,000 deaths were attributed to dengue. Real-time reverse transcription PCR (RT-qPCR) is the
Vilayouth Phimolsannousith +5 more
doaj +1 more source
Frontiers in MicrobiologyBackgroundThere are many similarities in the clinical manifestations of human norovirus and SARS-CoV-2 infections, and nucleic acid detection is the gold standard for diagnosing both diseases. In order to expedite the identification of norovirus and SARS-
Hua-Wei Yi +15 more
doaj +1 more source