Results 101 to 110 of about 495,188 (306)

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Analysis of ZAP70 expression in adult acute lymphoblastic leukaemia by real time quantitative PCR

Molecular Cytogenetics, 2012
Background ZAP70 gene expression is associated with poor prognosis in B-cell lymphoproliferative disorders especially chronic lymphocytic leukaemia (CLL) but its role in adult B-ALL has not been established.
Chakupurakal Geothy, Bell Andrew, Griffiths Mike, Wandroo Farooq, Moss Paul   +4 more
doaj   +1 more source

WetLab-2 RT-qPCR procedure v2

, 2017
Procedure used for RT-qPCR in the WetLab-2 ...
Jimmy Jung, Macarena Parra
openaire   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Diagnostic value of reverse transcription-quantitative PCR in skin pus specimens for HIV-negative talaromycosis marneffei

Guangxi Yike Daxue xuebao
Objective To evaluate the diagnostic value of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) detection of skin pus specimens in HIV-negative patients with talaromycosis marneffei (TSM).
Lan HUANG, Qihua HUANG, Dongyan ZHENG, Weixuan WU, Kaisu PAN, Yanqing ZHENG, Wanqing LIAO, Cunwei CAO   +7 more
doaj   +1 more source

Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms

Frontiers in Genetics, 2018
MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3′-end length variants
Charlotte Nejad, Charlotte Nejad, Geneviève Pépin, Geneviève Pépin, Mark A. Behlke, Michael P. Gantier, Michael P. Gantier   +6 more
doaj   +1 more source

Visualization of positive and negative sense viral RNA for probing the mechanism of direct-acting antivirals against hepatitis C virus [PDF]

, 2019
RNA viruses are highly successful pathogens and are the causative agents for many important diseases. To fully understand the replication of these viruses it is necessary to address the roles of both positive-strand RNA ((+)RNA) and negative-strand RNA ((
Bassit, Leda C, Huber, Andrew D, Ji, Juan, Lan, Shuiyun, Liu, Dandan, Michailidis, Eleftherios, Ndongwe, Tanyaradzwa P, Puray-Chavez, Maritza N, Ralston, Robert, Rice, Charles M, Saeed, Mohsan, Sarafianos, Stefan G, Schinazi, Raymond F, Tedbury, Philip R   +13 more
core   +2 more sources

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Detection of Chikungunya Virus RNA in Oral Fluid and Urine: An Alternative Approach to Diagnosis?

Viruses
To evaluate whether oral fluids (OF) and urine can serve as alternative, non-invasive samples to diagnose chikungunya virus (CHIKV) infection via RT-qPCR, we employed the same RNA extraction and RT-qPCR protocols on paired serum, OF and urine samples ...
Leile Camila Jacob-Nascimento, Moyra M. Portilho, Rosângela O. Anjos, Patrícia S. S. Moreira, Christine Stauber, Scott C. Weaver, Uriel Kitron, Mitermayer G. Reis, Guilherme S. Ribeiro   +8 more
doaj   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source