Results 101 to 110 of about 952,520 (310)

NAPRT loss promotes lung tumor initiation and growth through AKT signaling independently of NAD+ biosynthesis

Molecular Oncology, EarlyView.
Loss of NAPRT promotes lung tumor initiation and growth through a noncanonical mechanism, independent of its role in NAD+ biosynthesis. Mechanistically, NAPRT depletion activates the mTORC2‐driven AKT/β‐catenin signaling axis to enhance clonogenic and invasive phenotypes. Furthermore, lung‐specific Naprt deletion significantly increases tumor burden in
Myung Joon Oh, Ji Yun Jang, Yoon Jeon, Gi‐Cheon Kim, Hwanho Lee, Jooyoung Lee, Ju‐Hwa Kim, Hakhyun Kim, Minjee Kim, Ho‐Keun Kwon, Ho Lee, Hyun Seok Kim   +11 more
wiley   +1 more source

Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study

JMIR Public Health and Surveillance
BackgroundMany countries have applied the wastewater surveillance of the COVID-19 pandemic to their national public health monitoring measures.
Annika Länsivaara, Kirsi-Maarit Lehto, Rafiqul Hyder, Erja Sinikka Janhonen, Anssi Lipponen, Annamari Heikinheimo, Tarja Pitkänen, Sami Oikarinen   +7 more
doaj   +1 more source

Canine distemper virus detection by different methods of One-Step RT-qPCR

Ciência Rural, 2016
: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical ...
Claudia de Camargo Tozato, Vívian Ferreira Zadra, Caroline Rodrigues Basso, João Pessoa Araújo Junior   +3 more
doaj   +1 more source

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

Modified Polyadenylation-Based RT-qPCR Increases Selectivity of Amplification of 3′-MicroRNA Isoforms

Frontiers in Genetics, 2018
MicroRNA (miRNA) detection by reverse transcription (RT) quantitative real-time PCR (RT-qPCR) is the most popular method currently used to measure miRNA expression. Although the majority of miRNA families are constituted of several 3′-end length variants
Charlotte Nejad, Charlotte Nejad, Geneviève Pépin, Geneviève Pépin, Mark A. Behlke, Michael P. Gantier, Michael P. Gantier   +6 more
doaj   +1 more source

RT-qPCR data and aligned RIP sequences

, 2017
RT-qPCR data and aligned RIP ...
Steve J. Perlman (8037953), Ballinger, Matthew J., Matthew J. Ballinger (3319482), Perlman, Steve J.   +3 more
core   +1 more source

RT-qPCR validation.

, 2021
Scatter plot shows gene expression fold changes calculated from RT-qPCR data and RNA-seq data. The correlation coefficient (R2) value is = 0.98. The fold changes are from the comparison between the R-treated and the W-treated C.
Theerayut Toojinda (4018604), Atchara Paemanee (171376), Wanapinun Nawae (669298), Sithichoke Tangphatsornruang (399948), Nattapol Narong (11767446), Wirulda Pootakham (680893), Yanisa Ketngamkum (11767449), Kanokwan Romyanon (11767452), Thippawan Yoocha (680898)   +8 more
core   +1 more source

Human GAPDH RT-qPCR v1 [PDF]

, 2018
RNA from matched FFPE, PFPE and cryo preserved human tissues, stored for up to seven years at 22°C and 4°C, examined for integrity and usability in quantitative RT-PCR
openaire   +1 more source

p190A/ARHGAP35 and p190B/ARHGAP5 proteins in endometrial cancer: a novel cancer‐relevant paralog interplay

Molecular Oncology, EarlyView.
This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault, Capucine Heraud, Mélissa Correia de Oliveira, Véronique Neaud, Valérie Valesco, Valérie Prouzet‐Mauleon, Béatrice Turcq, Jean‐William Dupuy, Anne‐Aurélie Raymond, Sabrina Croce, Frédéric Saltel, Valérie Lagrée, Violaine Moreau   +12 more
wiley   +1 more source

RT-qPCR validation.

, 2019
(A) RT-qPCR validation of six miRNAs selected from the miRNA sequencing data. N = 6 per group, *P P < 0.05 vs. control group.
Cancan Lai (6954593), Hao Chen (5190), Weiwei Bao (5484116), Mingfang Huang (496505), Xiaobiao Zou (6954590), Zhurong Luo (496500), Zhiqing Wang (830081), Donglin Liu (24711), Ping Tan (1356951)   +8 more
core   +1 more source