Results 101 to 110 of about 795,270 (311)

Canine distemper virus detection by different methods of One-Step RT-qPCR

Ciência Rural, 2016
: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical ...
Claudia de Camargo Tozato, Vívian Ferreira Zadra, Caroline Rodrigues Basso, João Pessoa Araújo Junior   +3 more
doaj   +1 more source

Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

The EuroBiotech Journal, 2021
A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2.
Tuncel Gulten, Ergoren Mahmut Cerkez, Baddal Buket, Tulay Pinar, Arikan Ayse, Guler Emrah, Ozverel Cenk Serhan, Kaya Suer H., Sayan Murat, Sanlidag Tamer,   +10 more
doaj   +1 more source

[Considerations for normalisation of RT-qPCR in oncology].

Annales de biologie clinique, 2008
Gene expression analysis has many applications in the management of cancer, including diagnosis, prognosis, and therapeutic care. In this context, the reverse transcription quantitative polymerase chain reaction (RT-qPCR) has become the "gold standard" for mRNA quantification.
Ho-Pun-Cheung, Alexandre, Cellier, Dominic, Lopez-Crapez, Evelyne   +2 more
openaire   +2 more sources

RT-qPCR Validation of transcriptomic data in Karishma cultivar. [PDF]

, 2019
(A) R-based heat map of 10 selected DEGs for RT-qPCR. (B) Relative expression levels of selected ten genes and 18S as an internal reference detected by RT-qPCR in control and symptomatic leaves.
Lukas A. Mueller (235813), Imran Amin (61194), Shahid Mansoor (219615), Muhammad Asif (466310), Bharat Mishra (3539426), Rubab Zahra Naqvi (6312032), M. Shahid Mukhtar (183563), Susan Strickler (4662394), Syed Shan-e-Ali Zaidi (2805160)   +8 more
core   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study

JMIR Public Health and Surveillance
BackgroundMany countries have applied the wastewater surveillance of the COVID-19 pandemic to their national public health monitoring measures.
Annika Länsivaara, Kirsi-Maarit Lehto, Rafiqul Hyder, Erja Sinikka Janhonen, Anssi Lipponen, Annamari Heikinheimo, Tarja Pitkänen, Sami Oikarinen   +7 more
doaj   +1 more source

Analysis of ZAP70 expression in adult acute lymphoblastic leukaemia by real time quantitative PCR

Molecular Cytogenetics, 2012
Background ZAP70 gene expression is associated with poor prognosis in B-cell lymphoproliferative disorders especially chronic lymphocytic leukaemia (CLL) but its role in adult B-ALL has not been established.
Chakupurakal Geothy, Bell Andrew, Griffiths Mike, Wandroo Farooq, Moss Paul   +4 more
doaj   +1 more source

RNA Extraction and RT-qPCR v1

, 2022
This protocol is used to extract RNA from mouse brain tissue and quantify with RT-qPCR.
openaire   +1 more source

Bland-Altman plots of PRN vs. MN-RT-qPCR assays. [PDF]

, 2019
Neutralization assays were performed with low-passage Edmonston MV using a panel of human serum samples (N = 136). The concentrations were log transformed prior to the analysis.
Susette Audet (7251428), Esmeralda Alvarado-Facundo (436103), Judy A. Beeler (7251434), William J. Moss (7251431)   +3 more
core   +1 more source

Inhibition of cyclin‐dependent kinases 12/13 using CT7439 as a treatment for colorectal cancer with CDK12 upregulation

Molecular Oncology, EarlyView.
The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington, Divya L. Dayanidhi, Mohammad Zokaasadi, John B. Mantyh, Pelumi D. Olawuni, Gabrielle Rupprecht, Jeremy M. Force, Shannon McCall, Ashwani K. Bahl, David S. Hsu, Jason A. Somarelli   +10 more
wiley   +1 more source