Results 121 to 130 of about 795,270 (311)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

RT-qPCR analysis of SoRL2a. [PDF]

a-e, RT-qPCR analysis of SoRL2a in male and female plants from the dioecious line 03–009, and monoecious plants from line NIL-M; f, RT-qPCR analysis of SoRL2a in leaves, and female and male flowers of monoecious plants.
Yutaka Suzuki (1379), Atsushi Toyoda (24202), Takayuki Osabe (6933074), Babil Pachakkil (9943091), Keisuke Tanaka (810441), Akane Haseda (3316443), Yuki Sudo (163292), Hideki Hirakawa (103059), Kaoru Yamano (18018230), Keisuke Iwabuchi (18018233), Yasuyuki Onodera (3316440)   +10 more
core   +1 more source

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]

, 2012
Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Wagner, Carola, Pfaffl, Michael W., Meyer, Swanhild U., Kaiser, Sebastian, Thirion, Christian   +4 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Unmasking The Hidden Clues: Shedding Light On The Potential Of Novel Biomarkers In Endometrial Cancer, Comparing RT-Qpcr And Immunohistochemical Analysis In Clinical Specimens

Journal of Rawalpindi Medical College
Objective: The objective of this study was to compare the diagnostic accuracy of RT-qPCR in endometrial cancer using novel biomarkers in clinical specimens, keeping immunohistochemical analysis as the gold standard.
Sampana Fatima, Ayman Sabora, Iqra Anwar, Seher Naeem, Muhammad Abrar   +4 more
doaj   +1 more source

RT-qPCR test results of 10 unigenes in Acipenser sinensis. [PDF]

, 2019
RT-qPCR test results of 10 unigenes in Acipenser ...
Binzhong Wang (5788106), Jianbo Jian (642712), Yong Gao (29929), Kan Xiao (623589), Xueqing Liu (426267), Jing Yang (13969), Hejun Du (1958428), Jianwei Chen (281404), Jinquan Xia (3454094), Lei Chen (54296)   +9 more
core   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Specificity of MN-RT-qPCR assay using polyclonal antisera. [PDF]

, 2019
Specificity of MN-RT-qPCR assay using polyclonal antisera.
Susette Audet (7251428), Esmeralda Alvarado-Facundo (436103), Judy A. Beeler (7251434), William J. Moss (7251431)   +3 more
core   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source