Results 121 to 130 of about 952,520 (310)

RT-qPCR validation of DEGs and DEPs.

, 2019
A shown the informations of the 16 candidated genes. B shown the result of the RT-qPCR validation of the candidated genes.
Aiying Lei (3514988), Rui Wang (52434), Wanwen Liang (3514985), Ming Chen (115604), Qiong Yang (28822), Ting Huang (190932), Yu Liu (6938), Liping Li (120326)   +7 more
core   +1 more source

RT-qPCR results.

, 2023
RT-qPCR average Cq for 20 DSLD horse skin samples and 18 control horse skin samples. The Cq values were average of total 20 DSLD horse skin samples and 18 normal horse skin samples. Each sample was run twice in triplicates.
Jennifer Hope Roberts (16548590), Jaroslava Halper (16548602), Karen Blumenshine (16548596), Amy McLean (16548593), Florent David (7932980), Eva Müller-Alander (16548599), Jian Zhang (1682)   +6 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

RT-qPCR validation.

, 2016
Sugarcane unigenes selected for RT-qPCR analysis of 5-DAI and 200-DAI samples: longifolia-like protein (comp200950_c0_seq1); auxin transporter (comp205699_c0_seq1); SAM (comp194455_c0_seq1); invertase (comp201528_c0_seq1); trehalose 6P synthase ...
Silvana Creste (753890), Karen S. Aitken (753888), Alessandra C. Palhares (3095286), João P. Kitajima (3095289), Claudia B. Monteiro-Vitorello (466165), Patricia D. C. Schaker (753880), Marie-Anne Van Sluys (55590), Lucas M. Taniguti (753879), Leila P. Peters (753882), Maria L. C. Vieira (753891)   +9 more
core   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

RT-qPCR Validation of transcriptomic data in Karishma cultivar.

, 2019
(A) R-based heat map of 10 selected DEGs for RT-qPCR. (B) Relative expression levels of selected ten genes and 18S as an internal reference detected by RT-qPCR in control and symptomatic leaves.
Lukas A. Mueller (235813), Imran Amin (61194), Shahid Mansoor (219615), Muhammad Asif (466310), Bharat Mishra (3539426), Rubab Zahra Naqvi (6312032), M. Shahid Mukhtar (183563), Susan Strickler (4662394), Syed Shan-e-Ali Zaidi (2805160)   +8 more
core   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

RT-PCR and qPCR primers of examined lncRNA genes.

, 2017
RT-PCR and qPCR primers of examined lncRNA genes.
Jianbo Fu (3836437), Fengxin Gao (3836434), Xiaoning Zhang (369283), Peng Zhang (2071), Yuqing Wang (110531), Xuhui Zeng (169847)   +5 more
core   +1 more source

RT-qPCR Detection of Senescence-Associated Circular RNAs [PDF]

, 2016
Primary cells that reach the end of their replicative potential, encounter sublethal stress, or experience the activation of certain oncogenes cease proliferation and enter a state of long-term growth arrest named senescence. The senescent process has been implicated in a variety of age-related diseases and also in the pathogenesis of cancer ...
Amaresh C, Panda, Kotb, Abdelmohsen, Myriam, Gorospe   +2 more
openaire   +2 more sources