Results 141 to 150 of about 917,459 (311)

A Magneto‐Mechanically Activated Nerve Guidance Conduit Promotes Peripheral Nerve Regeneration via TIMP1‐Mediated Membrane Tension Transfer

Advanced Functional Materials, EarlyView.
This study presents a magneto‐mechanical strategy that incorporates FP@MSCs into an aligned PCL/GelMA nerve guidance conduit. Magnetic stimulation increases membrane tension in FP@MSCs, triggering cytoskeletal remodeling, Schwann cell‐like differentiation, and TIMP1 secretion. TIMP1 activates ITGB1/CD63–FAK signaling in NE‐4C cells, increasing membrane
Xinyu Zhu, Yumin Wu, Xiang Gao, Tianlu Wang, Wanting Jia, Xueying Mei, Huanzhong Ji, Zongxi Wu, Yujie Liang, Xiang Li, Kan Li, Jingyuan Li, Guangsen Zheng, Guiqing Liao, Siyong Gao   +14 more
wiley   +1 more source

Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential

Advanced Healthcare Materials, EarlyView.
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz, Manuel P. Kainz, Niklas Haider, Theresa Rienmüller, Kerstin Lenk, Rupert Kargl, Michele Terzano, Gerhard Sommer, Gerhard A. Holzapfel, Julia Fuchs   +9 more
wiley   +1 more source

Comparison of MN-RT-qPCR and PRN results.

, 2019
Comparison of MN-RT-qPCR and PRN results.
Susette Audet (7251428), Esmeralda Alvarado-Facundo (436103), Judy A. Beeler (7251434), William J. Moss (7251431)   +3 more
core   +1 more source

RT-qPCR (primary cells) v1

This protocol describes a qPCR-based method for analyzing Atp13a4 expression in primary astrocytes and neurons. Using a 96-well format, reactions are prepared with Fast SYBR Green Master Mix, specific forward and reverse primers, and nuclease-free water.
Shiyi Wang, Sarah van Veen
openaire   +1 more source

Engineered Microvascular Model of the Blood–Brain–Tumor Barrier Reveals Endothelial Remodeling in Diffuse Midline Glioma

Advanced Healthcare Materials, EarlyView.
We developed a patient‐derived, functional microfluidic model of the diffuse midline glioma (DMG) blood–brain–tumor barrier (BBTB) comprised of endothelial cells, astrocytes, pericytes, and tumor cells. The system forms perfusable microvasculature, reveals the BBTB retains vascular integrity, identifies DMG‐specific transcriptomic changes distinct from
Kimberly R. Bennett, Yann S. Vanrobaeys, Tessy Teboh, Zakiya Smith, Anderson Scott, Cynthia Hajal, Paula T. Hammond, Joelle P. Straehla   +7 more
wiley   +1 more source

RT-PCR and qPCR primers of examined coding genes.

, 2017
RT-PCR and qPCR primers of examined coding genes.
Jianbo Fu (3836437), Fengxin Gao (3836434), Xiaoning Zhang (369283), Peng Zhang (2071), Yuqing Wang (110531), Xuhui Zeng (169847)   +5 more
core   +1 more source

New Method for Lawsonia intracelullaris Quantification Based on Optical Density by Spectrophotometry

Microorganisms
Studies investigating the pathogenesis of Lawsonia intracellularis often require bacterial quantification in suspension. However, due to the organism’s fastidious growth requirements—being both intracellular and microaerophilic—traditional quantification
Mirtha E. Suarez-Duarte, Ricardo P. Laub, Renato L. Santos, Carlos E. R. Pereira, Talita P. Resende, Matheus D. Araujo, Paula A. Correia, Jessica C. R. Barbosa, Roberto M. C. Guedes   +8 more
doaj   +1 more source

Conjugated Porphyrin Polymer With Superior Charge Transfer for Photodynamic‐Immunomodulatory Prevention of CIED Infection in Diabetes

Advanced Healthcare Materials, EarlyView.
Under NIR irradiation, PTCPP generates ROS to eliminate MRSA biofilms and promote M1 macrophage polarization for phagocytosis. After bacterial clearance, the material is phagocytosed by macrophages, shifting their polarization to M2 phenotype via metabolic reprogramming, which synergistically enhances anti‐infection therapy and wound healing.
Honghao Ma, Jinguo Xu, Rongrong Gu, Chuanhui Dong, Te Chen, Yi Mu, Jing Zhu, Yating Wang, Wen Wang, Yingying Zhang, Hongyu Ma, Xiaochen Wang, Sheng Ye, Cheng Cheng   +13 more
wiley   +1 more source

RT-qPCR analysis of SoRL2a.

a-e, RT-qPCR analysis of SoRL2a in male and female plants from the dioecious line 03–009, and monoecious plants from line NIL-M; f, RT-qPCR analysis of SoRL2a in leaves, and female and male flowers of monoecious plants.
Yutaka Suzuki (1379), Atsushi Toyoda (24202), Takayuki Osabe (6933074), Babil Pachakkil (9943091), Keisuke Tanaka (810441), Akane Haseda (3316443), Yuki Sudo (163292), Hideki Hirakawa (103059), Kaoru Yamano (18018230), Keisuke Iwabuchi (18018233), Yasuyuki Onodera (3316440)   +10 more
core   +1 more source

RT-PCR and qPCR primers of examined lncRNA genes.

, 2017
RT-PCR and qPCR primers of examined lncRNA genes.
Jianbo Fu (3836437), Fengxin Gao (3836434), Xiaoning Zhang (369283), Peng Zhang (2071), Yuqing Wang (110531), Xuhui Zeng (169847)   +5 more
core   +1 more source