Results 141 to 150 of about 952,520 (310)

Linear-hairpin variable primer RT-qPCR for MicroRNA

Chemical Science, 2019
Here, we present a highly specific, sensitive and cost-effective system to quantify microRNA (miRNA) expression based on two-step RT-qPCR with EvaGreen detection chemistry, called linear-hairpin variable primer RT-qPCR.
Lin Lan, Qiuping Guo, Hemin Nie, Chang Zhou, Qingyun Cai, Jin Huang, Xiangxian Meng   +6 more
openaire   +3 more sources

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Rapid, sensitive and effective diagnostic tools for foot-and-mouth disease virus in Africa

Onderstepoort Journal of Veterinary Research, 2014
Speed is paramount in the diagnosis of highly infectious diseases, such as foot-and-mouth disease (FMD), as well as for emerging diseases; however, simplicity is required if a test is to be deployed in the field.
Christopher J. Kasanga, Wataru Yamazaki, Valerie Mioulet, Donald P. King, Misheck Mulumba, Ezekia Ranga, Jimis Deve, Cornelius Mundia, Patrick Chikungwa, Laureta Joao, Philemon N. Wambura, Mark M. Rweyemamu   +11 more
doaj   +1 more source

Specificity of MN-RT-qPCR assay using polyclonal antisera.

, 2019
Specificity of MN-RT-qPCR assay using polyclonal antisera.
Susette Audet (7251428), Esmeralda Alvarado-Facundo (436103), Judy A. Beeler (7251434), William J. Moss (7251431)   +3 more
core   +1 more source

1 Step RT- QPCR v1

description of how to do a 1 step rt ...
Chelsie Steele, Yujie Fan
openaire   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Dactolisib outperforms Rapamycin in potentiating apoptosis induced by XPO1, Bcl‐2 and Mcl‐1 blockade in preclinical glioblastoma models

FEBS Open Bio, EarlyView.
The PAM pathway and its impact on cellular metabolism. Color‐coded key symbols represent Rapamycin (R, orange, targeting mTOR) and Dactolisib (D, green, targeting PI3K/mTOR), indicating their sites of action. Created with BioRenders.com. Glioblastoma resists therapy through interconnected survival programs.
Kai Zhao, Leonie Meyer, Madita Braun, Katharina Otte, Celine Bitter, Maite Pawlik, Hartmann Raifer, Frederik Helmprobst, Axel Pagenstecher, Hans Urban, Michael C. Burger, Michael W. Ronellenfitsch, Joachim P. Steinbach, Jarmila Jedelská, Shamim Bagheri, Behrooz H. Yousefi, Pietro Di Fazio, Markus Luster, Andreas H. Mahnken, Andreas Neubauer, Andreas Burchert, Christopher Nimsky, Jörg W. Bartsch, Tillmann Rusch   +23 more
wiley   +1 more source

Digestive enzymes RT-qPCR

, 2017
The raw data (expressed as Ct-values) from RT-qPCR on genes for digestive proteases and amylases and maltases, measured in larvae of Control and Selected populations, developing at the poor diet at two stages, (early and late 3rd instar).
van der Meer, Jan R., Jan R. van der Meer (1331262), Tadeusz J. Kawecki (334590), Berra Erkosar (5017292), Kolly, Sylvain, Kawecki, Tadeusz J., Sylvain Kolly (691615), Erkosar, Berra   +7 more
core   +1 more source

Blood RNA Biomarker Signatures for Early Diagnosis and Prognosis in Ischemic and Hemorrhagic Stroke: The IBIS‐CT1 Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the expression of nine blood RNA biomarkers in a clinical trial based on genes previously identified in an experimental monkey model of stroke for diagnosis feasibility and prognostication. Methods IBIS‐CT1 was a prospective longitudinal study enrolling patients with ischemic stroke (IS) or intracerebral hemorrhage (ICH ...
Salomé Retailleau, Marie Bruguet, Irina Viakhireva‐Dovganyuk, François‐Mathias Merrien, Denis Marechal, Aurore Jourdain, Philippe Goas, Jordan Coris, Julien Asselineau, Maëlys Consigny, Gérald Le Gac, Serge Timsit   +11 more
wiley   +1 more source

Chronic Myeloid Leukaemia with isolated massive thrombocytosis and BCR‐ABL1 detection failure using RT‐MLPA (positive RT‐qPCR)

eJHaem, 2021
Lucie Coster, Véronique Mansat‐De MAS, Laetitia Largeaud, Sophie Voisin, Jill Corre, Martin Gauthier   +5 more
doaj   +1 more source