Results 151 to 160 of about 952,520 (310)

RT-qPCR target and reference gene primers for RNAseq validation.

, 2018
RT-qPCR target and reference gene primers for RNAseq validation.
Candice Chu (4976636), Cynthia Balog-Alvarez (3476054), Xue Yu (275844), James J. Cai (266155), Mary Nabity (4976633), Candice Brinkmeyer-Langford (3476048), Joe N. Kornegay (520803)   +6 more
core   +1 more source

RT-qPCR (cell lines) v1

This protocol describes the use of RT-qPCR to assess msAtp13a4 mRNA expression following lentiviral knockdown in C8-D1A cells.
Sarah van Veen, Joris Van Asselberghs, Peter Vangheluwe   +2 more
openaire   +1 more source

Integrated PANoptosis Profiling Identifies Immunosuppressive Subtypes and a Prognostic Signature With Functional Validation of MLKL in Glioblastoma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian, Minghui Zhao, Yuanbo Hu, Kaiyue Wang, Haiguang Liu, Zetong Bai, Kebin Zheng   +6 more
wiley   +1 more source

PCGF1 Promotes the Tumorigenesis of Malignancies Through Upregulating CCDC34 in Glioma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Polycomb group factor 1 (PCGF1) and coiled‐coil domain‐containing protein 34 (CCDC34) have been implicated in tumorigenesis in multiple malignancies. However, their biological functions and prognostic significance in glioma remain poorly defined.
Yuanbing Chen, Jianbing Xiong, Ziran Ou, Tianhao Zhao, Hui Li, Jun Huang, Zhi Tian, Wuyang Cao   +7 more
wiley   +1 more source

RT-qPCR analysis of SoRL2a.

a-e, RT-qPCR analysis of SoRL2a in male and female plants from the dioecious line 03–009, and monoecious plants from line NIL-M; f, RT-qPCR analysis of SoRL2a in leaves, and female and male flowers of monoecious plants.
Yutaka Suzuki (1379), Atsushi Toyoda (24202), Takayuki Osabe (6933074), Babil Pachakkil (9943091), Keisuke Tanaka (810441), Akane Haseda (3316443), Yuki Sudo (163292), Hideki Hirakawa (103059), Kaoru Yamano (18018230), Keisuke Iwabuchi (18018233), Yasuyuki Onodera (3316440)   +10 more
core   +1 more source

RT-qPCR.

, 2019
mRNA expression of induced-pluripotent stem cell markers OCT4 (A), SOX2 (B), NANOG (C), KLF4 (D) and c-MYC (E) detected by RT-qPCR. Data displayed as the fold-change of gene expression in tumor samples relative to their patient-matched normal colon ...
Swee T. Tan (5120171), Tinte Itinteang (5120168), Susrutha K. Wickremesekera (5645231), Matthew J. Munro (7354253), Lifeng Peng (709111), Reginald W. Marsh (5645237)   +5 more
core   +1 more source

RT-qPCR (primary cells) v1

This protocol describes a qPCR-based method for analyzing Atp13a4 expression in primary astrocytes and neurons. Using a 96-well format, reactions are prepared with Fast SYBR Green Master Mix, specific forward and reverse primers, and nuclease-free water.
Shiyi Wang, Sarah van Veen
openaire   +1 more source

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

New Method for Lawsonia intracelullaris Quantification Based on Optical Density by Spectrophotometry

Microorganisms
Studies investigating the pathogenesis of Lawsonia intracellularis often require bacterial quantification in suspension. However, due to the organism’s fastidious growth requirements—being both intracellular and microaerophilic—traditional quantification
Mirtha E. Suarez-Duarte, Ricardo P. Laub, Renato L. Santos, Carlos E. R. Pereira, Talita P. Resende, Matheus D. Araujo, Paula A. Correia, Jessica C. R. Barbosa, Roberto M. C. Guedes   +8 more
doaj   +1 more source

Remote Activation of Wnt Signaling and Cell Proliferation by E‐Cadherin Magnetomechanical Stimulation

Advanced Functional Materials, EarlyView.
A pioneering magnetogenetic toolkit enables non‐invasive, precise spatiotemporal modulation of intracellular Wnt/β‐catenin signaling. Functionalized magnetic nanoparticles (MNPs@E/EC15) target cellular E‐cadherins to generate localized magnetomechanical forces under low‐intensity magnetic fields.
Christian Castro‐Hinojosa, Pablo Martínez‐Vicente, Lucía G. Recaredo, Susel Del Sol‐Fernández, Pilar Gomollón‐Zueco, Yilian Fernández‐Afonso, Raluca M. Fratila, María Moros   +7 more
wiley   +1 more source