Results 131 to 140 of about 952,520 (310)
Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound
FEBS Open Bio, EarlyView.We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda +4 more
wiley +1 more source
RT-qPCR primers used in the study entitled 'Tissue-specific profiling of age-dependent miRNAomic changes in Caenorhabditis elegans'
Yidong Shen (6957248)
core +1 more source
Comparison of MN-RT-qPCR and PRN results.
, 2019 Comparison of MN-RT-qPCR and PRN results.
Susette Audet (7251428) +3 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
RT-qPCR test results of 10 unigenes in Acipenser sinensis.
, 2019 RT-qPCR test results of 10 unigenes in Acipenser ...
Binzhong Wang (5788106) +9 more
core +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
RT-PCR and qPCR primers of examined coding genes.
, 2017 RT-PCR and qPCR primers of examined coding genes.
Jianbo Fu (3836437) +5 more
core +1 more source
Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR
, 2019 The identification of NADPH oxidase (NOX) isoforms in tissues is essential for interpreting experiments and for next step decisions regarding cell lines, animal models, and targeted drug design. Two basic methods, immunoblotting and reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), are important to monitor NOX protein and ...
Diebold, Becky BA +7 more
openaire +4 more sources
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Bland-Altman plots of PRN vs. MN-RT-qPCR assays.
, 2019 Neutralization assays were performed with low-passage Edmonston MV using a panel of human serum samples (N = 136). The concentrations were log transformed prior to the analysis.
Susette Audet (7251428) +3 more
core +1 more source