Isoform-level gene signature improves prognostic stratification and accurately classifies glioblastoma subtypes. [PDF]
, 2014 Molecular stratification of tumors is essential for developing personalized therapies. Although patient stratification strategies have been successful; computational methods to accurately translate the gene-signature from high-throughput platform to a ...
Bi, Yingtao +5 more
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RNA-Based Detection Does not Accurately Enumerate Living Escherichia coli O157:H7 Cells on Plants [PDF]
, 2016 The capacity to distinguish between living and dead cells is an important, but often unrealized, attribute of rapid detection methods for foodborne pathogens. In this study, the numbers of enterohemorrhagic Escherichia coli O157:H7 after inoculation onto
Anne-Laure Moyne +2 more
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Large-Scale RT-qPCR Diagnostics for Seed Potato Certification [PDF]
Potato Research, 2021 AbstractEvery year, Agroscope examines nearly 300,000 tubers for the presence of viruses, as regulated for the certification of seed potatoes intended for Swiss growers. Since 2016, this examination has been performed via RT-qPCR on dormant tubers directly after harvest.
Olivier Schumpp +7 more
openaire +2 more sources
Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]
, 2012 Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Kaiser, Sebastian +4 more
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Validation of extracellular miRNA quantification in blood samples using RT‐qPCR
FASEB BioAdvances, 2019 Extracellular microRNAs (miRs) have been proposed as important blood‐based biomarkers for several diseases. Contrary to proteins and other RNA classes, miRs are stable and easily detectable in body fluids.
Maria Fauth +4 more
doaj +1 more source
Commercial stocks of SARS-CoV-2 RNA may report low concentration values, leading to artificially increased apparent sensitivity of diagnostic assays [PDF]
, 2020 In response to the rapidly evolving COVID-19 pandemic, the U.S. Food and Drug Administration (FDA) has rapidly issued 49 emergency use authorizations (EUAs) for SARS-CoV-2 in vitro diagnostic test-kits. A critical metric in the performance evaluation for
Ismagilov, Rustem F., Jue, Erik
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International Journal of Statistics in Medical Research, 2012 We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.
Perkins, James R. +2 more
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Journal of Integrative Agriculture, 2014 Two microRNA (miRNA) quantification methods, namely, poly(A) reverse transcription (RT)-quantitative real-time polymerase chain reaction (qPCR) and stem-loop RT-qPCR, have been developed for quantifying miRNA expression. In the present study, five miRNAs,
Ran HAN +3 more
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Effect of tungstate on acetate and ethanol production by the electrosynthetic bacterium Sporomusa ovata [PDF]
, 2016 Additional file 1: Table S1.
Ammam, Fariza +3 more
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, 2021 Protocol-RT-qPCR to the paper: Viral mapping in COVID-19 deceased in the Augsburg autopsy series of the first wave: a multiorgan and multimethodological approach Full organ mapping of patients with COVID-19: an autopsy study
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