Results 31 to 40 of about 952,520 (310)

Improving the standardization of mRNA measurement by RT-qPCR [PDF]

open access: yesBiomolecular Detection and Quantification, 2018
Human health and safety depend on reliable measurements in medical diagnosis and on tests that support the selection and evaluation of therapeutic intervention and newly discovered molecular biomarkers must pass a rigorous evaluation process if they are to be of benefit to patients.
Rebecca Sanders   +3 more
openaire   +3 more sources

Results of RT-qPCR-positive blood samples.

open access: yes, 2022
Results of RT-qPCR-positive blood samples.
Tadashi Furuyama (8084270)   +6 more
core   +1 more source

Stem‐Loop RT‐qPCR for miRNAs [PDF]

open access: yesCurrent Protocols in Molecular Biology, 2011
AbstractThis unit presents a specific and sensitive quantitative reverse‐transcription PCR (RT‐qPCR) method for measuring individual microRNAs (miRNAs) in tissue or cultured cells. miRNAs are 17 to 24 nucleotides (nt) in length. Standard and quantitative PCR methods require a template that is at least two times the length of either of the specific ...
openaire   +2 more sources

RT-qPCR Data

open access: yes, 2023
RT-qPCR raw data for cytokine expression using the ddct ...
Samir Casseb (757504)
core   +1 more source

Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

open access: yesBMC Infectious Diseases, 2020
Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya   +4 more
doaj   +1 more source

Saliva as alternative to naso-oropharyngeal swab for SARS-CoV-2 detection by RT-qPCR: a multicenter cross-sectional diagnostic validation study

open access: yesScientific Reports, 2022
Saliva has been demonstrated as feasible alternative to naso-oropharyngeal swab (NOS) for SARS-CoV-2 detection through reverse transcription quantitative/real-time polymerase chain reaction (RT-qPCR).
Michael L. Tee   +9 more
doaj   +1 more source

Impact of Capsid and Genomic Integrity Tests on Norovirus Extraction Recovery Rates

open access: yesFoods, 2023
Human norovirus (HuNoV) is the leading pathogen responsible for food-borne illnesses. However, both infectious and non-infectious HuNoV can be detected by RT-qPCR.
Philippe Raymond   +2 more
doaj   +1 more source

Analysis of RT-qPCR Data

open access: yesInternational Journal of Statistics in Medical Research, 2012
We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.
Perkins, James R.   +2 more
openaire   +2 more sources

INVESTIGAÇÃO DE PARECHOVIRUS, AICHIVIRUS E COSAVIRUS EM AMOSTRAS DE GASTROENTERITE AGUDA NA REGIÃO NORTE E NORDESTE DO BRASIL

open access: yesBrazilian Journal of Infectious Diseases, 2023
Introdução/objetivos: As gastroenterites agudas (GEA) são doenças que se constituem como a segunda maior causa de morte em crianças de todo mundo, especialmente em crianças menores de cinco anos, ocorrendo principalmente em países de baixa e média renda.
Ana Luiza da Mota Raminho   +5 more
doaj   +1 more source

RT-qPCR and qPCR assay details and performance for the viral targets.

open access: yes, 2023
RT-qPCR and qPCR assay details and performance for the viral targets.
Kata Farkas (3174156)   +7 more
core   +1 more source

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