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Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]
, 2012 Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Van Peer, Gert +2 more
core +1 more source
High-throughput microfluidic single-cell RT-qPCR [PDF]
Proceedings of the National Academy of Sciences, 2011 A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run.
Adam K, White +8 more
openaire +2 more sources
Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]
, 2013 Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra +6 more
core +2 more sources
Impact of Capsid and Genomic Integrity Tests on Norovirus Extraction Recovery Rates
Foods, 2023 Human norovirus (HuNoV) is the leading pathogen responsible for food-borne illnesses. However, both infectious and non-infectious HuNoV can be detected by RT-qPCR.
Philippe Raymond +2 more
doaj +1 more source
The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing [PDF]
, 2011 Background The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo, mainly relating to inefficient delivery and non-specific effects of short RNA ...
Mike Herbert +4 more
core +2 more sources
BMC Infectious Diseases, 2020 Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya +4 more
doaj +1 more source
Scientific Reports, 2022 Saliva has been demonstrated as feasible alternative to naso-oropharyngeal swab (NOS) for SARS-CoV-2 detection through reverse transcription quantitative/real-time polymerase chain reaction (RT-qPCR).
Michael L. Tee +9 more
doaj +1 more source
Brazilian Journal of Infectious Diseases, 2023 Introdução/objetivos: As gastroenterites agudas (GEA) são doenças que se constituem como a segunda maior causa de morte em crianças de todo mundo, especialmente em crianças menores de cinco anos, ocorrendo principalmente em países de baixa e média renda.
Ana Luiza da Mota Raminho +5 more
doaj +1 more source
Stem‐Loop RT‐qPCR for miRNAs [PDF]
Current Protocols in Molecular Biology, 2011 AbstractThis unit presents a specific and sensitive quantitative reverse‐transcription PCR (RT‐qPCR) method for measuring individual microRNAs (miRNAs) in tissue or cultured cells. miRNAs are 17 to 24 nucleotides (nt) in length. Standard and quantitative PCR methods require a template that is at least two times the length of either of the specific ...
openaire +2 more sources
COVID-19 diagnosis by RT-qPCR in alternative specimens
Memorias do Instituto Oswaldo Cruz, 2021 BACKGROUND The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent ...
Cássia Cristina Alves Gonçalves +22 more
doaj +1 more source