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Stem‐Loop RT‐qPCR for miRNAs [PDF]
Current Protocols in Molecular Biology, 2011 AbstractThis unit presents a specific and sensitive quantitative reverse‐transcription PCR (RT‐qPCR) method for measuring individual microRNAs (miRNAs) in tissue or cultured cells. miRNAs are 17 to 24 nucleotides (nt) in length. Standard and quantitative PCR methods require a template that is at least two times the length of either of the specific ...
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, 2023 RT-qPCR raw data for cytokine expression using the ddct ...
Samir Casseb (757504)
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BMC Infectious Diseases, 2020 Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya +4 more
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Scientific Reports, 2022 Saliva has been demonstrated as feasible alternative to naso-oropharyngeal swab (NOS) for SARS-CoV-2 detection through reverse transcription quantitative/real-time polymerase chain reaction (RT-qPCR).
Michael L. Tee +9 more
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Impact of Capsid and Genomic Integrity Tests on Norovirus Extraction Recovery Rates
Foods, 2023 Human norovirus (HuNoV) is the leading pathogen responsible for food-borne illnesses. However, both infectious and non-infectious HuNoV can be detected by RT-qPCR.
Philippe Raymond +2 more
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International Journal of Statistics in Medical Research, 2012 We give a brief overview of the necessary steps in the analysis of real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) data. We cover determination of amplification efficiency, background correction, normalization, quality control, and statistical analysis.
Perkins, James R. +2 more
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Brazilian Journal of Infectious Diseases, 2023 Introdução/objetivos: As gastroenterites agudas (GEA) são doenças que se constituem como a segunda maior causa de morte em crianças de todo mundo, especialmente em crianças menores de cinco anos, ocorrendo principalmente em países de baixa e média renda.
Ana Luiza da Mota Raminho +5 more
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Results of RT-qPCR-positive blood samples.
, 2022 Results of RT-qPCR-positive blood samples.
Tadashi Furuyama (8084270) +6 more
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Validation of extracellular miRNA quantification in blood samples using RT‐qPCR
FASEB BioAdvances, 2019 Extracellular microRNAs (miRs) have been proposed as important blood‐based biomarkers for several diseases. Contrary to proteins and other RNA classes, miRs are stable and easily detectable in body fluids.
Maria Fauth +4 more
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