Results 11 to 20 of about 952,520 (310)
Tutorial: Guidelines for Single-Cell RT-qPCR [PDF]
Cells, 2021 Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape. Thanks to its precision, sensitivity, flexibility, and cost effectiveness, RT-qPCR has also found utility in advanced ...
Daniel Zucha +2 more
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An RT–qPCR Assay for Rapid Detection of Coxsackievirus A4 [PDF]
VirusesCoxsackievirus A4 (CV-A4) is a major cause of hand, foot, and mouth disease and herpangina in children, and has been linked to severe complications. However, current detection methods, including commercial RT-qPCR kits, suffer from false-negative and ...
Xiaofeng Qiu +3 more
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Primer PICKR: literature-mined scoring platform for robust RT–qPCR primers [PDF]
Nature CommunicationsReliable reverse-transcription quantitative PCR (RT–qPCR) depends on well-designed primers, yet undocumented or poorly validated sequences continue to compromise reproducibility. Existing resources catalog only modest sets of empirically verified primers
Thomas G. Molley +4 more
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Molecules and Cells: RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature ...
Dajeong Bong +2 more
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Rapid and accurate quantification of isomiRs by RT-qPCR
Scientific Reports, 2022 Currently, microRNAs (miRs) are annotated as a single defined sequence (canonical), even though high-throughput small RNA sequencing has identified miR isoforms (isomiRs) that differ from their canonical counterparts in length, sequence, or both. Here we
Sandra Franco +4 more
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COVID-19 diagnosis by RT-qPCR in alternative specimens [PDF]
Memorias do Instituto Oswaldo Cruz, 2021 BACKGROUND The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent ...
Cássia Cristina Alves Gonçalves +22 more
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RT-qPCR Testing of SARS-CoV-2: A Primer [PDF]
International Journal of Molecular Sciences, 2020 Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely ...
Stephen A. Bustin (16654086) +1 more
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Selection of stable reference genes for RT–qPCR in Salmo trutta
Aquaculture Reports, 2022 To identify suitable reference genes for Salmo trutta, real-time fluorescent quantitative PCR (RT–qPCR) technology was used to determine the expression of eight candidate genes in 11 tissues (heart, liver, spleen, head kidney, muscle, brain, gills, ovary,
Shuaijie Sun +10 more
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A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System. [PDF]
PLoS ONE, 2016 Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR).
Coralie Coudray-Meunier +5 more
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, 2023 The primer sequences for RT-qPCR targeting each indicated gene. Baf155, Brg1/Brm-associated factor 155; CISH, cytokine inducible SH2 containing protein; F, forward; Glut, glucose transporter; LDHA, lactate dehydrogenase A; MyHC, myosin heavy chain; Pgk ...
Ji-Yun Seo (400939) +13 more
core +2 more sources