Results 11 to 20 of about 917,459 (311)
Pathogens, 2021 Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the ...
Laurence Thirion +7 more
doaj +1 more source
Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses
Engineering, 2020 Human noroviruses (HuNoVs) are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide. As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus, detection is mainly dependent on molecular ...
Danlei Liu +6 more
doaj +1 more source
Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
Scientific Reports, 2023 Rift valley fever (RVF) is an important zoonotic disease caused by the Rift valley fever virus (RVFV) which can affect ruminants and humans. In this study, a comparison was done of the reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Changwoo Park +4 more
doaj +1 more source
SARS-CoV-2 nucleic acid shedding is variable for every person
Revista da Sociedade Brasileira de Medicina Tropical, 2021 INTRODUCTION: Considering the persistent positivity on RT-qPCR tests, the results of SARS-CoV-2 were monitored to evaluate the viral RNA shedding period.
Daiana de Lima-Morales +5 more
doaj +1 more source
RT-qPCR and ChIP-qPCR primers.
, 2021 RT-qPCR and ChIP-qPCR primers.
Hilary T. Brewis (11798006) +5 more
core +1 more source
Molekularbiologische Diagnostik von Pflanzenkrankkeiten
Julius-Kühn-Archiv, 2014 In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine +3 more
doaj +1 more source
RT-qPCR half-reaction optimization for the detection of SARS-CoV-2
Revista da Sociedade Brasileira de Medicina Tropical, 2021 INTRODUCTION: The main laboratory test for the diagnosis of coronavirus disease 2019 (COVID-19) is the reverse transcription real-time polymerase chain reaction (RT-qPCR). However, RT-qPCR is expensive because of the number of tests required. This study
Priscila Lamb Wink +7 more
doaj +1 more source
RT-qPCR-based confirmation of RNA-sequencing results using both LNA GapmeRs against TUG1.
, 2022 HUVECs were transfected with two LNA GapmeRs against TUG1—LNA TUG1_1 and LNA TUG1_2 –and LNA Ctrl and expression levels of (A) VAMP4, (B) TOR1AIP2, (C) KAT6B and (D) ABCA1 were measured after 48 hours by RT-qPCR. Expression is relative to P0 (n = 4; SEM;
Tamer Ali (632879) +14 more
core +1 more source
Improving the standardization of mRNA measurement by RT-qPCR [PDF]
Biomolecular Detection and Quantification, 2018 Human health and safety depend on reliable measurements in medical diagnosis and on tests that support the selection and evaluation of therapeutic intervention and newly discovered molecular biomarkers must pass a rigorous evaluation process if they are to be of benefit to patients.
Rebecca Sanders +3 more
openaire +3 more sources