Results 11 to 20 of about 495,188 (306)
Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection
International Journal of Infectious Diseases, 2021 Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated to diagnose several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. This study
Masato Inaba +7 more
doaj +1 more source
Pathogens, 2021 Toscana virus (TOSV) can cause central nervous system infections in both residents of and travelers to Mediterranean countries. Data mining identified three real-time RT-qPCR assays for detecting TOSV RNA targeting non-overlapping regions in the ...
Laurence Thirion +7 more
doaj +1 more source
Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses
Engineering, 2020 Human noroviruses (HuNoVs) are major foodborne pathogens that cause nonbacterial acute gastroenteritis worldwide. As the tissue-culture system for HuNoVs is not mature enough for routine detection of the virus, detection is mainly dependent on molecular ...
Danlei Liu +6 more
doaj +1 more source
Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
Scientific Reports, 2023 Rift valley fever (RVF) is an important zoonotic disease caused by the Rift valley fever virus (RVFV) which can affect ruminants and humans. In this study, a comparison was done of the reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
Changwoo Park +4 more
doaj +1 more source
BDNF Expression in Cortical GABAergic Interneurons [PDF]
, 2020 Brain-derived neurotrophic factor (BDNF) is a major neuronal growth factor that is widely expressed in the central nervous system. It is synthesized as a glycosylated precursor protein, (pro)BDNF and post-translationally converted to the mature form, (m ...
Barreda Tomás, Federico José +6 more
core +1 more source
SARS-CoV-2 nucleic acid shedding is variable for every person
Revista da Sociedade Brasileira de Medicina Tropical, 2021 INTRODUCTION: Considering the persistent positivity on RT-qPCR tests, the results of SARS-CoV-2 were monitored to evaluate the viral RNA shedding period.
Daiana de Lima-Morales +5 more
doaj +1 more source
Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]
, 2016 Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude +5 more
core +2 more sources
Molekularbiologische Diagnostik von Pflanzenkrankkeiten
Julius-Kühn-Archiv, 2014 In der Abteilung für Molekularbiologische Diagnose von Pflanzenkrankheiten der AGES werden molekularbiologische Diagnoseverfahren eingesetzt um gesetzliche Kontroll- und Vollzugsaufgaben zu erfüllen, aber auch um Untersuchungen auf pflanzliche ...
Grausgruber-Gröger, Sabine +3 more
doaj +1 more source
Analytic lymph node number establishes staging accuracy by occult tumor burden in colorectal cancer. [PDF]
, 2012 BACKGROUND AND OBJECTIVES: Recurrence in lymph node-negative (pN0) colorectal cancer suggests the presence of undetected occult metastases. Occult tumor burden in nodes estimated by GUCY2C RT-qPCR predicts risk of disease recurrence.
Barkun, Alan +4 more
core +2 more sources
RT-qPCR half-reaction optimization for the detection of SARS-CoV-2
Revista da Sociedade Brasileira de Medicina Tropical, 2021 INTRODUCTION: The main laboratory test for the diagnosis of coronavirus disease 2019 (COVID-19) is the reverse transcription real-time polymerase chain reaction (RT-qPCR). However, RT-qPCR is expensive because of the number of tests required. This study
Priscila Lamb Wink +7 more
doaj +1 more source