Results 21 to 30 of about 7,761,125 (307)

Reference genes identification for qRT-PCR normalization of gene expression analysis in Cucumis sativus under Meloidogyne incognita infection and Pseudomonas treatment

Frontiers in Plant Science, 2022
qRT-PCR is a common and key technical means to study gene expression in biological research. However, reliability and accuracy of quantification by qRT-PCR is entirely dependent on the identification of appropriate reference genes.
Tingting Ji, Si Ma, Meiting Liang, Xueyun Wang, Lihong Gao, Yongqiang Tian   +5 more
doaj   +1 more source

Linking Cytoscape and the corynebacterial reference database CoryneRegNet [PDF]

, 2008
Baumbach J, Apeltsin L. Linking Cytoscape and the corynebacterial reference database CoryneRegNet. BMC Genomics. 2008;9(1): 184.Background: Recently, the research community has seen an influx of data relating to transcriptional regulatory interactions of
Baumbach Jan, Jan Baumbach, Apeltsin, Leonard, Baumbach, Jan, Apeltsin Leonard, Leonard Apeltsin   +5 more
core   +1 more source

Selection and Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Athetis dissimilis (Lepidoptera: Noctuidae) Under Different Conditions

Frontiers in Physiology, 2022
Reference genes are the key to study gene expression patterns using quantitative real-time PCR (qRT-PCR). No studies on the reference genes of Athetis dissimilis, an important agricultural pest, have been reported.
Jinrong Tang, Jinrong Tang, Gemei Liang, Shaoqi Dong, Shuang Shan, Man Zhao, Xianru Guo   +6 more
doaj   +1 more source

Selection and Evaluation of mRNA and miRNA Reference Genes for Expression Studies (qPCR) in Archived Formalin-Fixed and Paraffin-Embedded (FFPE) Colon Samples of DSS-Induced Colitis Mouse Model

Biology, 2023
The choice of appropriate reference genes is essential for correctly interpreting qPCR data and results. However, the majority of animal studies use a single reference gene without any prior evaluation.
Ana Unkovič, Emanuela Boštjančič, Aleš Belič, Martina Perše   +3 more
doaj   +1 more source

Association of the AFF3 gene and IL2/IL21 gene region with juvenile idiopathic arthritis [PDF]

, 2010
Recent genetic studies have led to identification of numerous loci that are associated with susceptibility to autoimmune diseases. The strategy of using information from these studies has facilitated the identification of novel juvenile idiopathic ...
S Eyre, J Worthington, X Ke, P Martin, W Thomson, A Hinks, E Flynn, J Packham, A Barton, Martin, P., Barton, A., Gardner-Medwin, J. [CAPS and BSPAR study groups], UKRAG Consortium, Worthington, J., Thomson, W., Flynn, E., Packham, J., Hinks, A., British Society of Paediatric and Adolescent Rheumatology (BSPAR) Study Group, Eyre, S., Ke, X., Childhood Arthritis Prospective Study (CAPS)   +21 more
core   +1 more source

A Strategy for the Selection of RT-qPCR Reference Genes Based on Publicly Available Transcriptomic Datasets

Biomedicines, 2023
In the next-generation sequencing era, RT-qPCR is still widely employed to quantify levels of nucleic acids of interest due to its popularity, versatility, and limited costs. The measurement of transcriptional levels through RT-qPCR critically depends on
Alice Nevone, Francesca Lattarulo, Monica Russo, Giada Panno, Paolo Milani, Marco Basset, Maria Antonietta Avanzini, Giampaolo Merlini, Giovanni Palladini, Mario Nuvolone   +9 more
doaj   +1 more source

Selection of reference genes for gene expression studies in rats

Journal of Biotechnology, 2011
Selection of the most stable reference gene is critical for a reliable interpretation of gene expression data using RT-PCR. In order so, 17 commonly used genes were analyzed in Wistar rat duodenum, jejunum, ileum and liver following a fat gavage and at two time periods.
Martínez-Beamonte, R., Navarro, M. A., Larraga, A., Strunk, M., Barranquero, C., Acín, S., Guzman, M. A., Iñigo, P., Osada, J.   +8 more
openaire   +3 more sources

The choice of reference gene affects statistical efficiency in quantitative PCR data analysis

BioTechniques, 2013
Quantitative polymerase chain reaction (qPCR), a highly sensitive method of measuring gene expression, is widely used in biomedical research.
Yi Guo, Michael L. Pennell, Dennis K. Pearl, Thomas J. Knobloch, Soledad Fernandez, Christopher M. Weghorst   +5 more
doaj   +1 more source

Reference gene identification for normalisation of RT‐qPCR analysis in plasma samples of the rat middle cerebral artery occlusion model

Veterinary Medicine and Science, 2022
Objective In quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR) studies, the selection and validation of reference genes are crucial for the accurate analysis of MicroRNAs (miRNAs) expression. In this work, the optimal reference genes
Hui Zhou, Xin Yang, Jiayi Yu, Jingyi Xu, Ruiwen Zhang, Ting Zhang, Xijie Wang, Jing Ma   +7 more
doaj   +1 more source

Selection and Validation of miRNA Reference Genes by Quantitative Real-Time PCR Analysis in Paeonia suffruticosa

Horticulturae, 2023
The miRNA, a kind of endogenous non-coding small RNA, plays an essential role in regulation of gene expression in plants. Quantitative real-time PCR (qRT-PCR) assay is one of the most common methods used for quantification of miRNA expression, and levels
Chenjie Zhang, Chengwei Song, Linfeng Chen, Huili Ma, Yabing Zhang, Dalong Guo, Lili Guo, Xiaogai Hou   +7 more
doaj   +1 more source