Results 21 to 30 of about 2,311,393 (299)

Reference Gene Screening for Analyzing Gene Expression Across Goat Tissue [PDF]

Asian-Australasian Journal of Animal Sciences, 2013
Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating the changes in mRNA expression levels in cells and tissues. Selection of the proper reference genes is very important when calibrating the results of real-time quantitative PCR.
Yu Zhang, Xiao-Dong Zhang, Xing Liu, Yun-Sheng Li, Jian-Ping Ding, Xiao-Rong Zhang, Yun-Hai Zhang   +6 more
openaire   +3 more sources

Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)

Insects, 2022
Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) is a widely used tool for measuring gene expression; however, its accuracy relies on normalizing the data to one or more stable reference genes.
Ying-Na Pan, Ru-Na Zhao, Di Fu, Chun Yu, Chun-Ni Pan, Wei Zhou, Wen-Long Chen   +6 more
doaj   +1 more source

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]

, 2013
Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra, Deforce, Dieter, Heindryckx, Björn, O'Leary, Thomas, Van Neste, Christophe, Vandesompele, Jo, Vossaert, Liesbeth   +6 more
core   +2 more sources

Selection and Validation of Reliable Reference Genes for qRT-PCR Normalization of Bursaphelenchus xylophilus from Different Temperature Conditions and Developmental Stages

Applied Sciences, 2022
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a powerful technique for studying gene expression. The key to quantitative accuracy depends on the stability of the reference genes used for data normalization under different ...
Yajing Wu, Jiao Zhou, Yaning Liu, Yutong Gu, Hongxia Zhang, Faheem Ahmad, Guochang Wang, Lili Ren   +7 more
doaj   +1 more source

Reference materials (RMs) for analysis of the human factor II (prothrombin) gene G20210A mutation [PDF]

, 2005
The Scientific Committee of Molecular Biology Techniques (C-MbT) in Clinical Chemistry of the IFCC has initiated a joint project in co-operation with the European Commission, Joint Research Centre, Institute of Reference Materials and Measurements to ...
Cooper, S., Corbisier, Philippe, Gancberg, David, Gemmati, Donato, Halbmayer, Walter-Michael, Kitchen, Steve, Klein, Christoph L., Mannhalter, Christine, Marki-Zay, János, Melegh, Béla, Neumaier, Michael, Oldenburg, Johannes, Opplinger Leibundgut, Elisabeth, Reitsma, Pieter H., Rieger, Sandra, Schimmel, Heinz G., Spannagl, Michael, Tordai, Attilia, Tosetto, Alberto, Visvikis, Sophie, Zadro, Renata   +20 more
core   +4 more sources

Effective Alu repeat based RT-qPCR normalization in cancer cell perturbation experiments [PDF]

, 2013
Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories.
Beckers, Anneleen, De Brouwer, Sara, Kumps, Candy, Leonelli, Carina, Mestdagh, Pieter, Mets, Evelien, Pattyn, Filip, Rihani, Ali, Rondou, Pieter, Speleman, Franki, Van der Meulen, Joni, Van Maerken, Tom, Van Peer, Gert, Vandesompele, Jo   +13 more
core   +10 more sources

Visualizing Viral Infection In Vivo by Multi-Photon Intravital Microscopy

Viruses, 2018
Viral pathogens have adapted to the host organism to exploit the cellular machinery for virus replication and to modulate the host cells for efficient systemic dissemination and immune evasion.
Xaver Sewald
doaj   +1 more source

Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats

Animals, 2020
Brown adipose tissues have unique non-shivering thermogenesis functions, can be found in newborn ruminate animals, and then are gradually replaced by white adipose tissues in adulthood.
Linjie Wang, Xingyue Chen, Tianzeng Song, Xujia Zhang, Siyuan Zhan, Jiaxue Cao, Tao Zhong, Jiazhong Guo, Li Li, Hongping Zhang, Yan Wang   +10 more
doaj   +1 more source

Reference gene selection and RNA preservation protocol in the cat flea, Ctenocephalides felis, for gene expression studies [PDF]

, 2016
This work was supported by a Knowledge Transfer Network BBSRC Industrial Case (#414 BB/L502467/1) studentship in association Zoetis Inc.Peer ...
Baird, John, Bowman, Alan S, Campbell, Ewan M, McIntosh, Catriona H, Woods, Debra J., Zinser, Erich   +5 more
core   +1 more source

Gene Expression Commons: an open platform for absolute gene expression profiling. [PDF]

, 2012
Gene expression profiling using microarrays has been limited to comparisons of gene expression between small numbers of samples within individual experiments.
Bhattacharya, Deepta, Dill, David L, Ehrlich, Lauren IR, Fathman, John W, Inlay, Matthew A, Rossi, Derrick J, Sahoo, Debashis, Seita, Jun, Serwold, Thomas, Weissman, Irving L   +9 more
core   +2 more sources