Results 1 to 10 of about 7,389 (186)

Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.). [PDF]

open access: yesPLoS ONE, 2015
Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts.
Roberta Fogliatto Mariot   +7 more
doaj   +1 more source

Reference gene selections for real time quantitative PCR analysis of gene expression in different oat tissues and under salt stress

open access: yesBiologia Plantarum, 2020
Appropriate choice of reference genes for data normalization is of critical importance for accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene expression.
Z.L. DUAN, W.H. HAN, L. YAN, B. WU
doaj   +1 more source

Transcriptome-based validation of proper reference genes for reverse trascription quantitative PCR analysis of Sinocalycanthus chinensis

open access: yesBiologia Plantarum, 2020
Reverse transcription quantitative PCR is a widely used method to detect gene expressions. To obtain accurate expression results, the selection of proper reference genes is important and necessary.
C. ZHANG   +6 more
doaj   +1 more source

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

open access: yesBiologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato ‘Hawaii 7996’ interactions

open access: yesScientific Reports, 2021
Reverse transcription-quantitative PCR (RT-qPCR) is an analytical tool for gene expression quantification. Reference genes are not yet available for gene expression analysis during interactions of Ralstonia solanacearum with ‘Hawaii 7996’ (the most ...
Greecy M. R. Albuquerque   +7 more
doaj   +1 more source

Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)

open access: yesScientific Reports, 2021
Although reference genes have previously been used in the expression analysis of genes involved in sugarcane flowering they had not been experimentally validated for stability and consistency of expression between different samples over a wide range of ...
Paulo H. da Silva Santos   +7 more
doaj   +1 more source

Selection of reference genes for normalization of real-time PCR data in visceral adipose tissue of female rats on a fructose-enriched diet [PDF]

open access: yesArchives of Biological Sciences, 2012
Considering the association of increased fructose intake with the rise in metabolic syndrome and gender differences in its incidence, this study was aimed at evaluating and identifying the optimal reference genes for the relative quantification of ...
Elaković Ivana   +3 more
doaj   +1 more source

Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis. [PDF]

open access: yesPLoS ONE, 2015
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes.
Chunxiao Yang   +8 more
doaj   +1 more source

Gene Expression Studies in Formalin-Fixed Paraffin-Embedded Samples of Cutaneous Cancer: The Need for Reference Genes

open access: yesCurrent Issues in Molecular Biology, 2021
Formalin-fixed paraffin-embedded (FFPE) tumour samples may provide crucial data regarding biomarkers for neoplasm progression. Analysis of gene expression is frequently used for this purpose.
Omar García-Pérez   +3 more
doaj   +1 more source

Reference gene validation for RT-qPCR, a note on different available software packages. [PDF]

open access: yesPLoS ONE, 2015
An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is ...
Ward De Spiegelaere   +10 more
doaj   +1 more source

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