Results 11 to 20 of about 7,593 (185)

Stable Reference Genes for RT-qPCR in House Dust Mite <i>Dermatophagoides farinae</i> (Acari: Pyroglyphidae) at Different Developmental Stages. [PDF]

Ecol Evol
First systematic evaluation of reference genes across different developmental stages of Dermatophagoides farinae. RPL18 and RPS28 are identified as the two stable reference genes. Cytochrome P450 3A8 gene validates that these stable reference genes provide robust and consistent expression patterns.
Jiang F, Ge M, Jin Z, Zhou H, Zhang M, Guan T, Hu H, Yin J, Liu Y.   +8 more
europepmc   +2 more sources

Gene Expression Studies in Formalin-Fixed Paraffin-Embedded Samples of Cutaneous Cancer: The Need for Reference Genes

Current Issues in Molecular Biology, 2021
Formalin-fixed paraffin-embedded (FFPE) tumour samples may provide crucial data regarding biomarkers for neoplasm progression. Analysis of gene expression is frequently used for this purpose.
Omar García-Pérez, Leticia Melgar-Vilaplana, Elizabeth Córdoba-Lanús, Ricardo Fernández-de-Misa   +3 more
doaj   +1 more source

Evaluation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in Pyrus pyrifolia using different tissue samples and seasonal conditions.

PLoS ONE, 2014
We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α ...
Tsuyoshi Imai, Benjamin E Ubi, Takanori Saito, Takaya Moriguchi   +3 more
doaj   +1 more source

Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Cumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş, Şeyda Berk, Ayşe Nur Pektaş   +2 more
doaj   +1 more source

Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of septic patients

Heliyon, 2023
Objective: To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC).
Ruoyu Song, Shijun He, Yongbin Wu, Wanxin Chen, Jie Song, Yaxi Zhu, Huan Chen, Qianlu Wang, Shouman Wang, Sichuang Tan, Sipin Tan   +10 more
doaj   +1 more source

Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

Plant Methods, 2017
Background Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield.
Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro, Silvana Creste   +7 more
doaj   +1 more source

ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP Were Identified as Reference Genes in Neuroendocrine Lung Cancer via the nCounter Technology. [PDF]

PLoS ONE, 2016
Neuroendocrine lung cancer (NELC) represents 25% of all lung cancer cases and large patient collectives exist as formalin-fixed, paraffin-embedded (FFPE) tissue only.
Robert Fred Henry Walter, Robert Werner, Claudia Vollbrecht, Thomas Hager, Elena Flom, Daniel Christian Christoph, Jan Schmeller, Kurt Werner Schmid, Jeremias Wohlschlaeger, Fabian Dominik Mairinger   +9 more
doaj   +1 more source

Gene stability (S) values calculated by Normfinder.

, 2013
Gene stability (S) values calculated by Normfinder.
Hong Zhou (29614), Penglong Li (428329), Dong Han (199240), Jiantao Feng (428326), Gan Chen (428325), Lian Zhao (96144), Guoxing You (428327), Quanmei Sun (428328)   +7 more
core   +2 more sources

Selection of suitable reference genes for quantitative real-time polymerase chain reaction in human meningiomas and arachnoidea

BMC Research Notes, 2011
Findings At first 32 housekeeping genes were analyzed in six randomly chosen meningiomas, brain and dura mater using geNorm, NormFinder, Bestkeeper-1 software and the comparative ΔCt method.
Pfister Christina, Tatabiga Marcos S, Roser Florian   +2 more
doaj   +1 more source

A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model

BMC Genomics, 2017
Background In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D ...
Felix Schulze, Deeksha Malhan, Thaqif El Khassawna, Christian Heiss, Anja Seckinger, Dirk Hose, Angela Rösen-Wolff   +6 more
doaj   +1 more source