Results 41 to 50 of about 7,593 (185)

Expression Stability Analysis of Candidate References for Normalization of RT-qPCR Data Using RefSeeker R package

Bio-Protocol, 2023
When performing expression analysis either for coding RNA (e.g., mRNA) or non-coding RNA (e.g., miRNA), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used method.
Patrick Petersen, Joanna Lopacinska-Jørgensen, Claus Høgdall, Estrid Høgdall   +3 more
doaj   +1 more source

Dilution‐Driven Regulation of Biostimulatory Signaling in Calcium Hydroxylapatite–PRGF Systems: Implications for Early Dermal Remodeling

Journal of Cosmetic Dermatology, Volume 25, Issue 11, November 2026.
ABSTRACT Background Calcium hydroxylapatite (CaHA)‐based fillers act as both volumizing agents and bioactive scaffolds capable of inducing matrix synthesis and dermal remodeling. Their rheological properties and biological activity can be modulated via dilution strategies, expanding their applicability in regenerative and aesthetic medicine.
Eduardo Anitua, Roberto Tierno, Mohammad Hamdan Alkhraisat   +2 more
wiley   +1 more source

Examining the Impact of Neurodevelopmental Nicotine Exposure on Cortical Excitation/Inhibition Homeostasis Markers in a Cerebral Organoid Model of Schizophrenia

Journal of Neurochemistry, Volume 170, Issue 10, October 2026.
Control‐ and schizophrenia‐derived cerebral organoids received 0, 0.1, or 1 μmol/L nicotine and were analyzed at Day 120 using quantitative polymerase chain reaction and immunofluorescence. Schizophrenia organoids showed reduced inhibitory markers, increased GABA receptor and glutamatergic markers, and altered cholinergic signaling, whereas the effects
Kuralay Zhaksylyk, Mohammed H. Sarikahya, Enzo Pérez‐Valenzuela, Daniel B. Hardy, Walter J. Rushlow, Steven R. Laviolette   +5 more
wiley   +1 more source

Expression of the long noncoding RNA Neat1 in saliva, blood, and ventromedial hypothalamus of pigs exposed to psychosocial stressors

Physiological Reports, Volume 14, Issue 20, October 2026.
Abstract The long noncoding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) is a master regulator of transcriptional and posttranscriptional changes in stress responses. Here we assessed Neat1 lncRNA expression using quantitative PCR in peripheral biological fluids (saliva and blood) and a brain region that regulates stress and emotion ...
Dane Webster, Luoyang Ding, Song Zhang, Darryl D'Souza, Alan Tilbrook, Katelyn Tomas, Sarah Babington, Kate Plush, Dominique Blache, Archa Fox   +9 more
wiley   +1 more source

Candidate HKGs in NormFinder evaluation.

, 2014
Ranking of candidate HKGs by NormFinder for each brain structure and all structures together, as described by the total M-value. Lower stability values indicate more stable genes. A cut-off value of 0.15 was used.Candidate HKGs in NormFinder evaluation.
Juliana Cini Perry (640841), Jair Ribeiro Chagas (640842), Guilherme Silva Julian (640839), Renato Watanabe de Oliveira (640840), Sergio Tufik (74924)   +4 more
core   +1 more source

Heterogeneity of miRNAs in Salivary Gland Neoplasms: An Exploratory Pilot Study

Journal of Oral Pathology &Medicine, Volume 55, Issue 8, Page 906-916, September 2026.
ABSTRACT Introduction MicroRNAs (miRNAs) are small RNAs that have been associated with tumourigenesis and tumour progression and that play an important role in the pathogenesis of salivary gland neoplasms. The aim of this study was to evaluate the global miRNA expression profile in a sample of salivary gland neoplasms.
Dandara Andrade de Santana, Poliana Ramos Braga, Daniel Araki Ribeiro, Patricia Ramos Cury, Ana Carolina Velasco Pondé de Sena, Flávia Caló de Aquino Xavier, Fernando Augusto Soares, Iguaracyra Barreto de Araújo, Fabio Albuquerque Marchi, Valéria Souza Freitas, Cláudia Malheiros Coutinho‐Camillo, Jean Nunes dos Santos   +11 more
wiley   +1 more source

Identification and validation of reference genes for quantitative gene expression analysis under 409 and 415 nm antimicrobial blue light treatment

Frontiers in Molecular Biosciences
IntroductionReverse transcription quantitative real-time polymerase chain reaction Q7 (RT‒qPCR) is a commonly used tool for gene expression quantification.
Beata Kruszewska-Naczk, Mariusz Grinholc, Aleksandra Rapacka-Zdonczyk   +2 more
doaj   +1 more source

Testing reference genes for transcript profiling in Uromyces appendiculatus during urediospore infection of common bean.

PLoS ONE, 2020
Uromyces appendiculatus is a major pathogen on common bean. Like other rust fungi, it uses effectors to influence its host plant. Effectors are assumed to possess characteristic expression profiles, reflecting their activity during the infection process.
Tobias I Link
doaj   +1 more source

Reference gene selection for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus semilaevis) at different developmental stages, in various tissue types and on exposure to chemicals. [PDF]

PLoS ONE, 2014
Quantitative real time RT-PCR has been described as the most sensitive method for the detection of low abundance mRNA. To date, no reference genes have been screened in the half-smooth tongue sole (Cynoglossus semilaevis).
Conghui Liu, Nian Xin, Yi Zhai, Liming Jiang, Jieming Zhai, Quanqi Zhang, Jie Qi   +6 more
doaj   +1 more source

Normfinder analysis (ungrouped).

, 2019
Representative outputs of the Normfinder algorithm. Stability values (left to right: least stable to most stable) for the reference gene candidates are shown for the entire dataset or dystrophic or healthy samples alone (as indicated).
Dominic J. Wells (205562), Amber M. Finch (6278357), John C. W. Hildyard (4637164)   +2 more
core   +1 more source