Results 61 to 70 of about 7,593 (185)

Leukocyte count affects expression of reference genes in canine whole blood samples

BMC Research Notes, 2011
Background The dog is frequently used as a model for hematologic human diseases. In this study the suitability of nine potential reference genes for quantitative RT-PCR studies in canine whole blood was investigated.
Dekker Aldo, Rothuizen Jan, Brinkhof Bas, Piek Christine J, Penning Louis C   +4 more
doaj   +1 more source

Integrated miRNA–mRNA Analysis Reveals Obesity‐Driven Regulatory Networks in Human Visceral Adipose Tissue With and Without Type 2 Diabetes

Diabetes, Obesity and Metabolism, Volume 28, Issue 7, Page 5992-6008, July 2026.
ABSTRACT Aims Obesity is characterised by pathological alterations in visceral white adipose tissue (vWAT) that may contribute to the development of type 2 diabetes (T2D). While microRNAs (miRNAs) are key post‐transcriptional regulators, comprehensive human vWAT profiling across metabolic states remains limited.
Elsa Villa‐Fernández, Ana Victoria García, Laura Gallardo‐Nuell, Miguel García‐Villarino, Judit Fernández‐García, Aldara Martin Alonso, Claudia Lozano‐Aida, Lorena Suárez Gutiérrez, Pedro Pujante, Jessica Ares, Tomás González‐Vidal, Raquel Rodríguez Uría, Sandra Sanz Navarro, María Moreno Gijón, Lourdes María Sanz Álvarez, Estrella Olga Turienzo Santos, José Manuel Fernández‐Real, Mario Fernández Fraga, Elías Delgado, Carmen Lambert   +19 more
wiley   +1 more source

Ranking of oil palm candidate reference genes using NormFinder analysis.

, 2014
Ranking of oil palm candidate reference genes using NormFinder analysis.
PLOS ONE (2189197)
core   +2 more sources

Selection and validation of appropriate reference genes for RT–qPCR analysis of Nitraria sibirica under various abiotic stresses

BMC Plant Biology, 2022
Background Nitraria sibirica Pall. is a halophytic shrub with strong environmental adaptability that can survive in extremely saline-alkali and drought-impacted environments. Gene expression analysis aids in the exploration of the molecular mechanisms of
Aishuang Hu, Xiuyan Yang, Jianfeng Zhu, Xiuping Wang, Jiaxin Liu, Jiping Wang, Haiwen Wu, Huilong Zhang, Huaxin Zhang   +8 more
doaj   +1 more source

Normfinder stability analysis.

, 2014
Evaluation of gene expression stability by Normfinder for individual brain regions. All HKG candidates presented values that were lower than the cut-off value (0.15). HC, hippocampus; HT, hypothalamus; FC, frontal cortex; TC, temporal cortex.
Juliana Cini Perry (640841), Jair Ribeiro Chagas (640842), Guilherme Silva Julian (640839), Renato Watanabe de Oliveira (640840), Sergio Tufik (74924)   +4 more
core   +1 more source

Selection and validation of reference genes for real-time quantitative PCR in hyperaccumulating ecotype of Sedum alfredii under different heavy metals stresses. [PDF]

PLoS ONE, 2013
Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions.
Jian Sang, Xiaojiao Han, Mingying Liu, Guirong Qiao, Jing Jiang, Renying Zhuo   +5 more
doaj   +1 more source

Normfinder ranking of the stability values of candidate reference genes.

, 2015
Normfinder ranking of the stability values of candidate reference genes.
Andre Baumann (780982), Zdeněk Franta (83127), Rüdiger Lehmann (780983), Andreas Vilcinskas (68058), Annika Beckert (780984)   +4 more
core   +1 more source

Pancreatic Islet Cell Crosstalk: Insight Into α‐/β‐Cell Compensatory Mechanisms

Comprehensive Physiology, Volume 16, Issue 3, June 2026.
α‐cell remodeling compensates for impaired β‐cell function. Intra‐islet glucagon/GLP‐1 signaling is upregulated and becomes essential in prediabetic islets. Flexible receptor engagement reflects functional plasticity in hormonal control of insulin secretion. Created in BioRender. Plecita, L. (2026); https://BioRender.com/pgjjg3m.
Štěpánka Benáková, Blanka Holendová, Jurij Dolenšek, Monika Křivonosková, Andraž Stožer, Lydie Plecitá‐Hlavatá   +5 more
wiley   +1 more source

Optimal use of statistical methods to validate reference gene stability in longitudinal studies.

PLoS ONE, 2019
Multiple statistical approaches have been proposed to validate reference genes in qPCR assays. However, conflicting results from these statistical methods pose a major hurdle in the choice of the best reference genes. Recent studies have proposed the use
Venkat Krishnan Sundaram, Nirmal Kumar Sampathkumar, Charbel Massaad, Julien Grenier   +3 more
doaj   +1 more source

GeNorm and NormFinder expression stability analysis.

, 2013
Ranking of candidate reference genes based on expression stability values calculated by NormFinder and ...
Ailbhe M. McDermott (114313), Nicola Miller (84418), Michael J. Kerin (114334)   +2 more
core   +1 more source