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Scientific ReportsFew studies have investigated the subcellular effects of low temperature on gene expression in shrimp and most other crustaceans. Before gene expression analysis is conducted, suitable housekeeping genes (HKGs) must be confirmed to account for ...
Yen-Po Chen +4 more
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BMC MicrobiologyPseudomonas aeruginosa can efficiently degrades petroleum hydrocarbons, but its degradation rate is affected by hydrocarbon pollutant concentrations, which must be neither too high nor too low. To quantitatively analyze P.
Huan Wang +8 more
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Validation of reference genes aiming accurate normalization of qRT-PCR data in Dendrocalamus latiflorus Munro. [PDF]
PLoS ONE, 2014 BACKGROUND: Dendrocalamus latiflorus Munro distributes widely in subtropical areas and plays vital roles as valuable natural resources. The transcriptome sequencing for D.
Mingying Liu +4 more
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Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.
PLoS ONE, 2020 INTRODUCTION:Real-time polymerase chain reaction (RT-qPCR) is an important tool for analyzing gene expression. However, before analyzing the expression of target genes, it is crucial to normalize the reference genes, in order to find the most stable gene
Juliana Gracielle Gonzaga Gromboni +7 more
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Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties
Plants, 2023 miRNA plays an important role in plant growth and development and in response to various stresses. Quantitative real-time PCR (qRT-PCR) technology is often used to detect the expression level of miRNAs and genes by comparing with reference genes.
Jiajia Shen +4 more
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Current Issues in Molecular Biology, 2021 Formalin-fixed paraffin-embedded (FFPE) tumour samples may provide crucial data regarding biomarkers for neoplasm progression. Analysis of gene expression is frequently used for this purpose.
Omar García-Pérez +3 more
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Study of Some Reference Genes Expression Stability in Rice Using Real-Time PCR Method Under Biotic Stress [PDF]
مجله بیوتکنولوژی کشاورزی, 2019 Objective Gene expression studies by Real-Time PCR constitute a powerful tool to analyze the mechanisms underlying plant biotic-stress tolerance. One of the crucial steps of this technique is the selection and validation of reference genes to normalize ...
Hamidreza Ghorbani +2 more
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PLoS ONE, 2023 Background Anemia and retinopathy of prematurity (ROP) are common comorbidities experienced by preterm infants, yet the role of anemia on the pathogenesis of ROP remains unclear. Reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR) is a
Mandkhai Molomjamts +1 more
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Screening of Reference Genes for Quantitative Real-time PCR in Globba spp.
Guangdong nongye kexue【Objective】Screening for stable reference genes that are applicable to the study of different varieties of Globba spp. bracts and different tissue parts of G.
Lishan HUANG +8 more
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Journal of Integrative Agriculture, 2019 Mealybugs, such as Phenacoccus solenopsis, are highly sexually dimorphic. Winged adult males present such remarkable morphological differences from females that, to the untrained eye, conspecific adults of both sexes of P. solenopsis may be considered as
Lu-ying ZHENG +8 more
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