Results 21 to 30 of about 6,430 (223)

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

open access: yesBiologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

Reference gene selections for real time quantitative PCR analysis of gene expression in different oat tissues and under salt stress

open access: yesBiologia Plantarum, 2020
Appropriate choice of reference genes for data normalization is of critical importance for accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene expression.
Z.L. DUAN, W.H. HAN, L. YAN, B. WU
doaj   +1 more source

Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis. [PDF]

open access: yesPLoS ONE, 2015
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes.
Chunxiao Yang   +8 more
doaj   +1 more source

CDK7 as a Potential Exploratory Biomarker for Distinguishing Acute Myocardial. [PDF]

open access: yesClin Cardiol
Clinical Cardiology, Volume 49, Issue 6, June 2026.
Mehmood A, Tahir R.
europepmc   +2 more sources

Pairwise comparison using Pearson correlation coefficient (r) and BestKeeper vs reference genes index using BestKeeper.

open access: yes, 2023
Pairwise comparison using Pearson correlation coefficient (r) and BestKeeper vs reference genes index using BestKeeper.
Anurag Kumar (679730)   +9 more
core   +1 more source

I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues in Arabidopsis thaliana

open access: yesBiomolecules, 2023
Quantitative real-time polymerase chain reaction (qPCR) is a widely used method to analyse the gene expression pattern in the reproductive tissues along with detecting gene levels in mutant backgrounds.
Maria João Ferreira   +3 more
semanticscholar   +1 more source

Housekeeping gene evaluation with BestKeeper.

open access: yes, 2022
Housekeeping gene evaluation with BestKeeper.
Berta Levavi-Sivan (2456017)   +3 more
core   +1 more source

Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of patients with Takayasu arteritis [PDF]

open access: yesJichu yixue yu linchuang, 2021
Objective To validate proper reference genes for quantitative real-time polymerase chain reaction (RT-qPCR) used for comparing mRNA expression levels in Takayasu arteritis' (TAK) and healthy controls' (HC) peripheral blood mononuclear cells (PBMC ...
TIAN Yi-xiao, LI Jing
doaj  

Expression Stability Analysis of Candidate References for Normalization of RT-qPCR Data Using RefSeeker R package

open access: yesBio-Protocol, 2023
When performing expression analysis either for coding RNA (e.g., mRNA) or non-coding RNA (e.g., miRNA), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used method.
Patrick Petersen   +3 more
doaj   +1 more source

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae)

open access: yesFrontiers in Physiology, 2023
Introduction: Gene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects.
Qing-Fang Hou   +5 more
semanticscholar   +1 more source

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