Results 31 to 40 of about 6,430 (223)

Screening and Verification of Reference Genes for Analysis of Gene Expression in Garlic (Allium sativum L.) under Cold and Drought Stress

open access: yesPlants, 2023
The principal objective of this study was to screen and verify reference genes appropriate for gene expression evaluation during plant growth and development under distinct growth conditions.
Qi-Zhang Wang   +4 more
semanticscholar   +1 more source

Reference gene selection for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus semilaevis) at different developmental stages, in various tissue types and on exposure to chemicals. [PDF]

open access: yesPLoS ONE, 2014
Quantitative real time RT-PCR has been described as the most sensitive method for the detection of low abundance mRNA. To date, no reference genes have been screened in the half-smooth tongue sole (Cynoglossus semilaevis).
Conghui Liu   +6 more
doaj   +1 more source

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

open access: yesMethods and Protocols, 2023
Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes and recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used in data normalization differ between tissue ...
Andrea H. Dragon   +5 more
semanticscholar   +1 more source

A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model

open access: yesBMC Genomics, 2017
Background In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D ...
Felix Schulze   +6 more
doaj   +1 more source

Reference Genes for Expression Analyses by qRT-PCR in Phthorimaea operculella (Lepidoptera: Gelechiidae)

open access: yesInsects, 2022
Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is a momentous tool for calculating the expression levels of targeted genes across various experimental conditions.
Chen-Hui Shen   +6 more
semanticscholar   +1 more source

Analysis of reference genes by BestKeeper algorithm.

open access: yes, 2023
Analysis of reference genes by BestKeeper algorithm.
Shehla Izhar (16898619)   +10 more
core   +1 more source

Screening of reliable reference genes for the normalization of RT-qPCR in chicken gastrointestinal tract

open access: yesPoultry Science, 2023
The application of reverse transcription quantitative real-time PCR technology for the production of gene tissue expression profiles is a widely employed approach in molecular biology research.
Jianfei Chen   +4 more
semanticscholar   +1 more source

Expression stability of 11 candidate reference genes counted via BestKeeper.

open access: yes, 2022
Expression stability of 11 candidate reference genes counted via BestKeeper.
Hao Zhan (6853280)   +5 more
core   +1 more source

Expression stability evaluated by BestKeeper.

open access: yes, 2013
Expression stability evaluated by BestKeeper.
Hong Zhou (29614)   +7 more
core   +2 more sources

Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

open access: yesBMC Research Notes, 2019
Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome ...
Marcel Fernando da Silva   +7 more
doaj   +1 more source

Home - About - Disclaimer - Privacy