Results 31 to 40 of about 2,168 (134)

Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi

Scientific Reports, 2023
Naegleria gruberi is a free-living amoeboflagellate commonly found in freshwater and in soils around the world. It is a non-pathogenic relative of Naegleria fowleri, which is the etiologic agent of Primary Amoebic Meningoencephalitis (PAM).
Tania Martín-Pérez, Martina Köhsler, Julia Walochnik   +2 more
doaj   +1 more source

Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues

Frontiers in Oncology
BackgroundReal-time quantitative PCR (RT-qPCR), essential for gene expression and biomarker studies, requires stable endogenous reference genes (RGs) for normalization. This study aimed to identify consistently expressed RGs in breast cancer and adjacent
Yue Meng, Ya-Wen Wang, Zhi-Bao Xu, Zhong-Qi Qiao, Zhong-Qi Qiao, Can Liu, Yan-Duo Chen, Yao Xu, Kai Zhang   +8 more
doaj   +1 more source

Stable Reference Genes for RT‐qPCR in House Dust Mite Dermatophagoides farinae (Acari: Pyroglyphidae) at Different Developmental Stages

Ecology and Evolution, Volume 16, Issue 11, November 2026.
First systematic evaluation of reference genes across different developmental stages of Dermatophagoides farinae. RPL18 and RPS28 are identified as the two stable reference genes. Cytochrome P450 3A8 gene validates that these stable reference genes provide robust and consistent expression patterns.
Feng Jiang, Mingxia Ge, Zeyu Jin, Haoting Zhou, Mengmeng Zhang, Tong Guan, Haoyuan Hu, Jianhai Yin, Ying Liu   +8 more
wiley   +1 more source

Expression stability of candidate reference genes as determined by BestKeeper.

, 2022
Expression stability of candidate reference genes as determined by BestKeeper.
María Cristina Romero-Rodríguez (13205687), Belén Gaete Humada (13205681), Julio C. M. Iehisa (3947534), Aníbal Arévalos (13205675), Amiliana Pineda-Fretez (13205684), María Eugenia Flores Giubi (13205690), Man Mohan Kohli (12989922), Belén Ortíz (13205678), Adriana Orrego (13205669), María Cecilia Gavilán (13205672)   +9 more
core   +1 more source

Hepatic HIF2α Modulates Extra‐Hepatic Disease‐Associated Phenotypes During Metabolic Dysfunction‐Associated Steatotic Liver Disease

Comprehensive Physiology, Volume 16, Issue 5, October 2026.
High fat, high fructose, high cholesterol “GAN” diet feeding in mice induces MASLD, cardiac dysfunction, loss of lean mass, and hyperinsulinaemia. Hepatocyte‐specific deletion of HIF2α also causes cardiac dysfunction and loss of lean mass but prevents GAN induced hyperinsulinaemia and cardiac sympathetic dominance without preventing MASLD.
Lorenz M. W. Holzner, Rosa M. Korpershoek, Youguo Niu, Anna Cochrane, Paula M. Darwin, Julia Babuta, Areesha Nazeer, Cecilia Castro, Alice P. Sowton, Alice E. Knapton, Benjamin D. Thackray, Julian L. Griffin, Zoe Hall, Dino A. Giussani, Rob C. I. Wüst, Andrew J. Murray   +15 more
wiley   +1 more source

Ranking of candidate genes based from BestKeeper.

, 2018
Ranking of candidate genes based from BestKeeper.
Ghows Azzam (282065), Najat Dzaki (4976678)   +1 more
core   +1 more source

Cross‐Talk Between Histamine H2 Receptor and Glucocorticoid Receptor: Potential Implications in Acute Myeloid Leukemia Treatment

Pharmacology Research &Perspectives, Volume 14, Issue 5, October 2026.
ABSTRACT This study investigates the impact of histamine H2 receptor activation on glucocorticoid receptor (GR)‐mediated transcriptional activity, as well as its effects on leukemic cell proliferation and chemotherapy resistance. We demonstrate that activation of the histamine H2 receptor by its specific agonist, amthamine, enhances GR transcriptional ...
Valeria Torralba‐Agu, Natalia Fernández, Carina Shayo, Carlos Davio, C. Daniel Zappia, Federico Monczor   +5 more
wiley   +1 more source

Stability ranking of 20 candidate reference genes using geNorm,Normfinder and Bestkeeper.

, 2014
Notes: LP, leaf-expansion period; FS, the flower stage; GFS, the green fruit stage; RFS, the red fruit stages; RGS, the root growing after fruit stage. G,geNorm software; N, Normfinder software; B, Bestkeeper software.
Yu Zhao (104852), Minying Sun (660055), Linlin Zhu (514231), Qun Wang (102815), Michael Yang (660056), Jing Liu (38537)   +5 more
core   +1 more source

Influence of heat stress, sex and genetic groups on reference genes stability in muscle tissue of chicken. [PDF]

PLoS ONE, 2017
Quantitative RT-PCR is an important technique for assessing gene expression. However, a proper normalization of reference genes prior to expression analyses of target genes is necessary.
Haniel Cedraz de Oliveira, Antonio Amandio Pinto Garcia, Juliana Gracielle Gonzaga Gromboni, Ronaldo Vasconcelos Farias Filho, Carlos Souza do Nascimento, Amauri Arias Wenceslau   +5 more
doaj   +1 more source

Examining the Impact of Neurodevelopmental Nicotine Exposure on Cortical Excitation/Inhibition Homeostasis Markers in a Cerebral Organoid Model of Schizophrenia

Journal of Neurochemistry, Volume 170, Issue 10, October 2026.
Control‐ and schizophrenia‐derived cerebral organoids received 0, 0.1, or 1 μmol/L nicotine and were analyzed at Day 120 using quantitative polymerase chain reaction and immunofluorescence. Schizophrenia organoids showed reduced inhibitory markers, increased GABA receptor and glutamatergic markers, and altered cholinergic signaling, whereas the effects
Kuralay Zhaksylyk, Mohammed H. Sarikahya, Enzo Pérez‐Valenzuela, Daniel B. Hardy, Walter J. Rushlow, Steven R. Laviolette   +5 more
wiley   +1 more source