Results 51 to 60 of about 6,430 (223)

BestKeeper rankings.

, 2019
BestKeeper rankings.
Dominic J. Wells (205562), Amber M. Finch (6278357), John C. W. Hildyard (4637164)   +2 more
core   +1 more source

Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

Journal of Animal Science and Biotechnology, 2012
Background Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results.
McCulloch Ryan S, Ashwell Melissa S, O’Nan Audrey T, Mente Peter L   +3 more
doaj   +1 more source

Comparative Analysis of the Expression Profiles of Pathogenesis-Related Genes in Tomato Systemically Infected with Tobacco Mosaic and Cucumber Mosaic Viruses

International Journal of Plant Biology, 2023
In this study, we used RT-qPCR to examine how PR genes were expressed in model tomato (Solanum lycopersicum L.) plants that had been infected with TMV or CMV. Under greenhouse conditions, the indirect ELISA data showed that both viruses were detected for
Dalia G. Aseel, Sherien E. Sobhy, M. Samy, Esraa Hamdy, S. Behiry, A. Abdelkhalek   +5 more
semanticscholar   +1 more source

BestKeeper pair-wise correlation.

, 2017
BestKeeper pair-wise correlation.
Cristina Pirrone (3737356), Mario Cherubino (3737353), Silvia Palombella (3737350), Giovanni Bernardini (842729), Luigi Valdatta (3737359), Rosalba Gornati (842723)   +5 more
core   +1 more source

Identification of Reference Genes for Reverse Transcription-Quantitative PCR Analysis of Ginger Under Abiotic Stress and for Postharvest Biology Studies

Frontiers in Plant Science, 2022
Gene expression analysis largely improves our understanding of the molecular basis underpinning various plant biological processes. Stable reference genes play a foundational role during the normalization of gene expression levels.
Gang Li, Jiawei Ma, Junliang Yin, F. Guo, Keyong Xi, Peihua Yang, Xiaodong Cai, Qie Jia, Lulu Li, Yiqing Liu, Yong-Xing Zhu   +10 more
semanticscholar   +1 more source

Abundance, efficiency, and stability of reference transcript expression in a seasonal rodent: The Siberian hamster

PLoS ONE, 2022
Quantitative PCR (qPCR) is a common molecular tool to analyse the expression of transcripts in non-traditional animal models. Most animals experience tissue-specific seasonal changes in cell structure, growth, and cellular function. As a consequence, the
Calum Stewart, Timothy A. Liddle, Tyler J. Stevenson   +2 more
doaj   +2 more sources

Expression Stability of Reference Genes in Larvae of Spodoptera frugipferda Under Azadirachtin Stress by Real-Time Quantitative PCR Analysis

Guangdong nongye kexue
【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU, Yuting HUANG, Xuanyue YU, Cuiting LIU, Xinyi XIE, Hao SHEN, Jintian LIN   +6 more
doaj   +1 more source

Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi

Scientific Reports, 2023
Naegleria gruberi is a free-living amoeboflagellate commonly found in freshwater and in soils around the world. It is a non-pathogenic relative of Naegleria fowleri, which is the etiologic agent of Primary Amoebic Meningoencephalitis (PAM).
Tania Martín-Pérez, Martina Köhsler, Julia Walochnik   +2 more
doaj   +1 more source

BestKeeper analysis.

, 2019
Representative outputs of the BestKeeper algorithm. Coefficient of correlation values for the reference gene candidates are shown for the entire dataset, or dystrophic or healthy samples alone (as indicated), ranked (left to right) from least stable to ...
Dominic J. Wells (205562), Amber M. Finch (6278357), John C. W. Hildyard (4637164)   +2 more
core   +1 more source

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus

Frontiers in Microbiology
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective tools for studying gene expression. Inonotus obliquus is a rare and edible medicinal fungus.
Lulu Li, Xiaofang Guo, Shou-Ming Wang
semanticscholar   +1 more source