Results 41 to 50 of about 6,430 (223)

Verification of suitable and reliable reference genes for quantitative real-time PCR during adipogenic differentiation in porcine intramuscular stromal-vascular cells

Animal, 2016
Intramuscular fat (IMF) is an important trait influencing meat quality, and intramuscular stromal-vascular cell (MSVC) differentiation is a key factor affecting IMF deposition.
X. Li, K. Huang, F. Chen, W. Li, S. Sun, X.-E. Shi, G. Yang   +6 more
doaj   +1 more source

Selection and validation of reference genes for real-time quantitative PCR in hyperaccumulating ecotype of Sedum alfredii under different heavy metals stresses. [PDF]

PLoS ONE, 2013
Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions.
Jian Sang, Xiaojiao Han, Mingying Liu, Guirong Qiao, Jing Jiang, Renying Zhuo   +5 more
doaj   +1 more source

Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells

BMC Research Notes, 2013
Background The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this
Wei Ruoxin, Stewart Elizabeth Anne, Amoaku Winfried M   +2 more
doaj   +1 more source

Identification of stably expressed Internal Control Genes (ICGs) for normalization of expression data in liver of C57BL/6 mice injected with beta casomorphins.

PLoS ONE, 2023
In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk have drawn a lot of attention world over because of their proposed impact on human health.
Anurag Kumar, Monika Sodhi, Manishi Mukesh, Amandeep Kaur, Gaurav Bhakri, Vipul Chaudhary, Preeti Swami, Vishal Sharma, Ashok Kumar Mohanty, Ranjit S Kataria   +9 more
doaj   +1 more source

Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

Scientific Reports, 2021
Choroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for
Kim Hoa Ho, Annarita Patrizi
semanticscholar   +1 more source

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Frontiers in Plant Science, 2017
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang   +5 more
doaj   +1 more source

Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells

Adipocyte, 2023
Normalization is a crucial step in gene expression analysis to avoid misinterpretation. Reverse transcription-quantitative polymerase chain reaction was used to measure the expression of 10 candidate housekeeping genes in non-differentiated (ND) and ...
D. Cahyadi, T. Warita, Nanami Irie, Kana Mizoguchi, J. Tashiro, Y. Hosaka, K. Warita   +6 more
semanticscholar   +1 more source

Identification of a novel reference gene for apple transcriptional profiling under postharvest conditions. [PDF]

PLoS ONE, 2015
Reverse Transcription quantitative PCR (RT-qPCR) is one of the most important techniques for gene expression profiling due to its high sensibility and reproducibility.
Tatiane Timm Storch, Camila Pegoraro, Taciane Finatto, Vera Quecini, Cesar Valmor Rombaldi, César Luis Girardi   +5 more
doaj   +1 more source

Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression in Nicotiana benthamiana upon Single Infections by 11 Positive-Sense Single-Stranded RNA Viruses from Four Genera

Plants, 2023
Quantitative real-time PCR (RT-qPCR) is a widely used method for studying alterations in gene expression upon infections caused by diverse pathogens such as viruses.
Ge Zhang, Zhuo Zhang, Q. Wan, Huijie Zhou, Mengting Jiao, Hong-Ying Zheng, Yuwen Lu, Shaofei Rao, Guan-Wei Wu, Jianping Chen, Fei Yan, Jiejun Peng, Jian Wu   +12 more
semanticscholar   +1 more source

Screening and Validation of Appropriate Reference Genes for Real-Time Quantitative PCR under PEG, NaCl and ZnSO4 Treatments in Broussonetia papyrifera

International Journal of Molecular Sciences, 2023
Real-time quantitative PCR (RT-qPCR) has a high sensitivity and strong specificity, and is widely used in the analysis of gene expression. Selecting appropriate internal reference genes is the key to accurately analyzing the expression changes of target ...
Mengdi Chen, Zhengbo Wang, Ziyuan Hao, Hongying Li, Qi Feng, Xue Yang, Xiaojiao Han, Xi-Ping Zhao   +7 more
semanticscholar   +1 more source