Results 1 to 10 of about 11,356 (189)
Reference Gene Validation for Gene Expression Normalization in Canine Osteosarcoma: A geNorm Algorithm Approach [PDF]
BMC Veterinary Research, 2017 Background Quantitative PCR (qPCR) is a common method for quantifying mRNA expression. Given the heterogeneity present in tumor tissues, it is crucial to normalize target mRNA expression data using appropriate reference genes that are stably expressed ...
Gayathri Thevi Selvarajah +2 more
exaly +6 more sources
GeneChip, geNorm, and Gastrointestinal Tumors: Novel Reference Genes for Real-Time PCR [PDF]
Physiological Genomics, 2007 Accurate quantitation of target genes depends on correct normalization. Use of genes with variable tissue transcription ( GAPDH) is problematic, particularly in clinical samples, which are derived from different tissue sources. Using a large-scale gene database (Affymetrix U133A) data set of 36 gastrointestinal (GI) tumors and ...
Geeta N Eick, Mark Kidd, Boaz Nadler
exaly +3 more sources
A Simple Method to Assess Group Difference in RT-qPCR Reference Gene Selection Using GeNorm: The Case of the Placental Sex [PDF]
Scientific Reports, 2017 AbstractNormalization with proper reference genes is a crucial step in obtaining accurate mRNA expression levels in RT-qPCR experiments. GeNorm and NormFinder are two commonly used software packages that help in selecting the best reference genes, based on their expression stability.
Cathy Vaillancourt
exaly +7 more sources
Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues [PDF]
Frontiers in OncologyBackgroundReal-time quantitative PCR (RT-qPCR), essential for gene expression and biomarker studies, requires stable endogenous reference genes (RGs) for normalization. This study aimed to identify consistently expressed RGs in breast cancer and adjacent
Yue Meng +8 more
doaj +2 more sources
GenORM: Generalizable One-shot Rope Manipulation with Parameter-Aware Policy
CoRR, 2023 Due to the inherent uncertainty in their deformability during motion, previous methods in rope manipulation often require hundreds of real-world demonstrations to train a manipulation policy for each rope, even for simple tasks such as rope goal reaching, which hinder their applications in our ever-changing world.
So Kuroki +9 more
openaire +2 more sources
Cumhuriyet Science Journal, 2022 Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş +2 more
doaj +1 more source
Heliyon, 2023 Objective: To screen and validate reference genes suitable for gene mRNA expression study in peripheral blood mononuclear cells (PBMCs) between septic patients and healthy controls (HC).
Ruoyu Song +10 more
doaj +1 more source
Biologia Plantarum, 2020 Appropriate choice of reference genes for data normalization is of critical importance for accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene expression.
Z.L. DUAN, W.H. HAN, L. YAN, B. WU
doaj +1 more source
Biologia Plantarum, 2020 Reverse transcription quantitative PCR is a widely used method to detect gene expressions. To obtain accurate expression results, the selection of proper reference genes is important and necessary.
C. ZHANG +6 more
doaj +1 more source
Biologia Plantarum, 2020 Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj +1 more source