Results 11 to 20 of about 11,356 (189)
Stable Reference Genes for RT-qPCR in House Dust Mite <i>Dermatophagoides farinae</i> (Acari: Pyroglyphidae) at Different Developmental Stages. [PDF]
Ecol EvolFirst systematic evaluation of reference genes across different developmental stages of Dermatophagoides farinae. RPL18 and RPS28 are identified as the two stable reference genes. Cytochrome P450 3A8 gene validates that these stable reference genes provide robust and consistent expression patterns.
Jiang F +8 more
europepmc +2 more sources
LEMming: A Linear Error Model to Normalize Parallel Quantitative Real-Time PCR (qPCR) Data as an Alternative to Reference Gene Based Methods. [PDF]
PLoS ONE, 2015 Gene expression analysis is an essential part of biological and medical investigations. Quantitative real-time PCR (qPCR) is characterized with excellent sensitivity, dynamic range, reproducibility and is still regarded to be the gold standard for ...
Ronny Feuer +6 more
doaj +1 more source
Selection of Reference Genes for Quantitative Real-time PCR Analysis in Canine Mammary Tumors Using the GeNorm Algorithm [PDF]
Veterinary Pathology, 2006 Eleven reference genes (18s ribosomal ribonucleic acid [RNA], 28s ribosomal RNA, ubiquitin, beta-actin, glycerine aldehyde dehydrogenase, ATP-synthase subunit 5B, hydroxymethylbilane synthase, hypoxanthine-phosphoribosyl transferase, ribosomal protein L32, tryptophan 5monooxygenase activation protein (zeta polypeptide), and TATA-Box binding protein ...
B, Etschmann +4 more
openaire +2 more sources
Selection of reference genes for gene expression studies in Siberian Apricot (Prunus sibirica L.) Germplasm using quantitative real-time PCR. [PDF]
PLoS ONE, 2014 Quantitative real time reverse transcription polymerase chain reaction has been applied in a vast range of studies of gene expression analysis. However, real-time PCR data must be normalized with one or more reference genes.
Jun Niu +11 more
doaj +1 more source
Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.). [PDF]
PLoS ONE, 2015 Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts.
Roberta Fogliatto Mariot +7 more
doaj +1 more source
Frontiers in Microbiology, 2021 Alicyclobacillus acidoterrestris is a major concern in fruit juice industry due to its spoilage potential of acidic fruit juice. Quantifying the expression levels of functional genes by real-time quantitative polymerase chain reaction (RT-qPCR) is ...
Ning Zhao +6 more
doaj +1 more source
Scientific Reports, 2021 Reverse transcription-quantitative PCR (RT-qPCR) is an analytical tool for gene expression quantification. Reference genes are not yet available for gene expression analysis during interactions of Ralstonia solanacearum with ‘Hawaii 7996’ (the most ...
Greecy M. R. Albuquerque +7 more
doaj +1 more source
Genetic determinants of mycotoxin synthesis in genus Fusarium
Czech Journal of Food Sciences, 2011 The fungi of the genus Fusarium occur worldwide mainly on cereal grains. They are considered to be not only plant-pathogenic, but also the producers of secondary metabolites - mycotoxins.
Hana Havránková +2 more
doaj +1 more source
PLoS ONE, 2014 We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR) analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the literature such as β-Tubulin, Histone H3, Actin, Elongation factor-1α ...
Tsuyoshi Imai +3 more
doaj +1 more source
Cells, 2020 Gene expression analysis by quantitative real-time polymerase chain reaction (RT-qPCR) is routinely used in biomedical studies. The reproducibility and reliability of the data fundamentally depends on experimental design and data interpretation.
Joanna Brzeszczyńska +6 more
doaj +1 more source