Reference Gene Validation for RT–qPCR in PBMCs from Asthmatic Patients with or without Obesity
Methods and Protocols, 2022 Obesity is known to impair the efficacy of glucocorticoid medications for asthma control. Glucocorticoid-induced gene expression studies may be useful to discriminate those obese asthmatic patients who present a poor response to glucocorticoids.
Cesar Picado +2 more
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Animals, 2020 Brown adipose tissues have unique non-shivering thermogenesis functions, can be found in newborn ruminate animals, and then are gradually replaced by white adipose tissues in adulthood.
Hongping Zhang, Linjie Wang
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Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues [PDF]
Frontiers in OncologyBackgroundReal-time quantitative PCR (RT-qPCR), essential for gene expression and biomarker studies, requires stable endogenous reference genes (RGs) for normalization. This study aimed to identify consistently expressed RGs in breast cancer and adjacent
Yue Meng +8 more
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Frontiers in Microbiology, 2023 Pseudomonas aeruginosa is an opportunistic human pathogen notorious for its remarkable capacity of multi-drug resistance, and has become one of the most important model bacteria in clinical bacteriology research.
Lingning Meng +8 more
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Selection of appropriate reference genes in Apoe-/- mouse brain [PDF]
IBRO Neuroscience ReportsBackground: Reverse transcription quantitative real-time polymerase chain reaction is the gold standard for gene expression quantification. Yet, this method’s accuracy heavily depends on choosing appropriate reference genes for data normalization ...
Naz Mengi Camur +4 more
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BMC Plant Biology, 2022 Background Nitraria sibirica Pall. is a halophytic shrub with strong environmental adaptability that can survive in extremely saline-alkali and drought-impacted environments. Gene expression analysis aids in the exploration of the molecular mechanisms of
Aishuang Hu +8 more
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IDENTIFICATION OF RELIABLE REFERENCE GENES FOR GENE EXPRESSION STUDIES OF OIL PALM PLANTLETS USING NORMFINDER AND BESTKEEPER ALGORITHMS [PDF]
Journal of Oil Palm Research, 2019 Gene expression profiling analysis using real-time quantitative polymerase chain reaction (qRT-PCR) is commonly used due to its reliability and accuracy.
Muhamad Afiq A. H
openaire +2 more sources
Identification of stable internal reference genes for expression analysis in the liver and pancreas of diabetic mouse (Mus musculus L.) models under physiological, pathological and treatment conditions. [PDF]
PLoS ONEAccurate gene expression analysis via reverse transcription quantitative real-time PCR is vital for studying diabetes mellitus. Accurate and reliable normalization of RT-qPCR data, in accordance with the MIQE guidelines, demands a group of at least two ...
Van Diep Nong +7 more
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Characterization of reference genes in rare minnow, Gobiocypris rarus (Actinopterygii: Cypriniformes: Cyprinidae), in early postembryonic development and in response to EDCs treatment [PDF]
Acta Ichthyologica et Piscatoria, 2013 Background. Endocrine disrupting chemicals (EDCs) are natural and anthropogenic compounds discharged into the environment known to disrupt the endocrine system of humans and animals by mimicking functions of steroids in vivo.
F. Qin, L. Wang, S. Liu, Z. Wang
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Scientific Reports, 2021 Reverse transcription-quantitative PCR (RT-qPCR) is an analytical tool for gene expression quantification. Reference genes are not yet available for gene expression analysis during interactions of Ralstonia solanacearum with ‘Hawaii 7996’ (the most ...
Greecy M. R. Albuquerque +7 more
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