Results 51 to 60 of about 7,389 (186)

Identification and validation of reference genes for quantitative gene expression analysis under 409 and 415 nm antimicrobial blue light treatment

Frontiers in Molecular Biosciences
IntroductionReverse transcription quantitative real-time polymerase chain reaction Q7 (RT‒qPCR) is a commonly used tool for gene expression quantification.
Beata Kruszewska-Naczk, Mariusz Grinholc, Aleksandra Rapacka-Zdonczyk   +2 more
doaj   +1 more source

Testing reference genes for transcript profiling in Uromyces appendiculatus during urediospore infection of common bean.

PLoS ONE, 2020
Uromyces appendiculatus is a major pathogen on common bean. Like other rust fungi, it uses effectors to influence its host plant. Effectors are assumed to possess characteristic expression profiles, reflecting their activity during the infection process.
Tobias I Link
doaj   +1 more source

Integrated miRNA–mRNA Analysis Reveals Obesity‐Driven Regulatory Networks in Human Visceral Adipose Tissue With and Without Type 2 Diabetes

Diabetes, Obesity and Metabolism, Volume 28, Issue 7, Page 5992-6008, July 2026.
ABSTRACT Aims Obesity is characterised by pathological alterations in visceral white adipose tissue (vWAT) that may contribute to the development of type 2 diabetes (T2D). While microRNAs (miRNAs) are key post‐transcriptional regulators, comprehensive human vWAT profiling across metabolic states remains limited.
Elsa Villa‐Fernández, Ana Victoria García, Laura Gallardo‐Nuell, Miguel García‐Villarino, Judit Fernández‐García, Aldara Martin Alonso, Claudia Lozano‐Aida, Lorena Suárez Gutiérrez, Pedro Pujante, Jessica Ares, Tomás González‐Vidal, Raquel Rodríguez Uría, Sandra Sanz Navarro, María Moreno Gijón, Lourdes María Sanz Álvarez, Estrella Olga Turienzo Santos, José Manuel Fernández‐Real, Mario Fernández Fraga, Elías Delgado, Carmen Lambert   +19 more
wiley   +1 more source

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Frontiers in Plant Science, 2017
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang   +5 more
doaj   +1 more source

Reference gene selection for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus semilaevis) at different developmental stages, in various tissue types and on exposure to chemicals. [PDF]

PLoS ONE, 2014
Quantitative real time RT-PCR has been described as the most sensitive method for the detection of low abundance mRNA. To date, no reference genes have been screened in the half-smooth tongue sole (Cynoglossus semilaevis).
Conghui Liu, Nian Xin, Yi Zhai, Liming Jiang, Jieming Zhai, Quanqi Zhang, Jie Qi   +6 more
doaj   +1 more source

Expression stability for 11 candidate reference genes counted through NormFinder.

, 2022
Expression stability for 11 candidate reference genes counted through NormFinder.
Hao Zhan (6853280), Lin Liu (74495), Tianchong Wang (12475355), Xiujun Lu (8482328), Wanfeng Ai (12475358), Hanzhang Liu (12183474)   +5 more
core   +1 more source

Pancreatic Islet Cell Crosstalk: Insight Into α‐/β‐Cell Compensatory Mechanisms

Comprehensive Physiology, Volume 16, Issue 3, June 2026.
α‐cell remodeling compensates for impaired β‐cell function. Intra‐islet glucagon/GLP‐1 signaling is upregulated and becomes essential in prediabetic islets. Flexible receptor engagement reflects functional plasticity in hormonal control of insulin secretion. Created in BioRender. Plecita, L. (2026); https://BioRender.com/pgjjg3m.
Štěpánka Benáková, Blanka Holendová, Jurij Dolenšek, Monika Křivonosková, Andraž Stožer, Lydie Plecitá‐Hlavatá   +5 more
wiley   +1 more source

NormFinder and BestKeeper analysis of expression stability.

, 2013
Ranking of candidate reference genes based on stability values calculated by NormFinder (a) and BestKeeper (b) softwares for systemic PILO-model samples.
Maria Luisa Paçó-Larson (407357), Leila Rodrigues de Mendonça (449813), Marília Gabriela Pereira (449814), Norberto Garcia-Cairasco (304805), Tiago Gomes de Andrade (449815), Thalita Ewellyn Batista Sales Marques (449812), Daniel Leite Góes Gitaí (449816)   +6 more
core   +1 more source

Differential Transcriptome Analysis of Intrauterine UPD6pat Fetuses With Distinct Phenotypes

Molecular Genetics &Genomic Medicine, Volume 14, Issue 6, June 2026.
Paternal uniparental disomy of chromosome 6 (UPD6pat) is associated with transient neonatal diabetes mellitus and multisystem developmental anomalies, but the mechanisms underlying its phenotypic variability remain unclear. This study integrates amniotic fluid cfRNA transcriptome analysis and molecular diagnostics, identifying 372 differentially ...
Jiahui Yu, Yan Zhang, Wenlong Shen, Jun Ying, Huanzheng Li, Xiaohua Tang, Xujun He   +6 more
wiley   +1 more source

GeNorm and NormFinder expression stability analysis.

, 2013
Ranking of candidate reference genes based on expression stability values calculated by NormFinder and ...
Ailbhe M. McDermott (114313), Nicola Miller (84418), Michael J. Kerin (114334)   +2 more
core   +1 more source