Results 31 to 40 of about 7,593 (185)

Identification and Evaluation of Reference Genes for Accurate Transcription Normalization in Safflower under Different Experimental Conditions.

PLoS ONE, 2015
Safflower (Carthamus tinctorius L.) has received a significant amount of attention as a medicinal plant and oilseed crop. Gene expression studies provide a theoretical molecular biology foundation for improving new traits and developing new cultivars ...
Dandan Li, Bo Hu, Qing Wang, Hongchang Liu, Feng Pan, Wei Wu   +5 more
doaj   +1 more source

Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.)

BMC Plant Biology, 2010
Background Quantitative real-time PCR (qRT-PCR) is currently the most accurate method for detecting differential gene expression. Such an approach depends on the identification of uniformly expressed 'housekeeping genes' (HKGs).
Neutelings Godfrey, Hawkins Simon, Huis Rudy   +2 more
doaj   +1 more source

NormFinder analysis and established rankings.

, 2018
NormFinder analysis and established rankings.
Ghows Azzam (282065), Najat Dzaki (4976678)   +1 more
core   +1 more source

Bypassing hazard of housekeeping genes: Their evaluation in rat granule neurons treated with cerebrospinal fluid of Multiple Sclerosis subjects

Frontiers in Cellular Neuroscience, 2015
Gene expression studies employing real-time PCR has become an intrinsic part of biomedical research. Appropriate normalization of target gene transcript(s) based on stably expressed housekeeping genes is crucial in individual experimental conditions to ...
Deepali eMathur, Deepali eMathur, Gerardo eLopez-Rodas, Juan eUreña-Peralta, Bonaventura eCasanova, Francisco eCoret-Ferrer, Maria eBurgal-Marti   +6 more
doaj   +1 more source

Stability value calculated from NormFinder.

, 2014
Stability value calculated from NormFinder.
Benjamin E. Ubi (512506), Takaya Moriguchi (512508), Takanori Saito (512507), Tsuyoshi Imai (512505)   +3 more
core   +1 more source

Ranking of putative housekeeping genes according to NormFinder.

, 2020
Ranking of putative housekeeping genes according to NormFinder.
Boris Sagredo (745060), Adriana Bastias (8595282), Francisco Correa (3147018), Kristen Oviedo (8595285), Ruben Almada (8595288)   +4 more
core   +1 more source

Expression of Mineral and Urea Transport‐Related Genes in the Salivary Glands and Ruminal Epithelium of Young Goats Fed Nitrogen‐ and/or Calcium‐Reduced Diets

Journal of Animal Physiology and Animal Nutrition, EarlyView.
ABSTRACT An adequate supply of protein and minerals, particularly nitrogen (N) and calcium (Ca), is essential for ruminant growth and metabolic function. The rumen and salivary glands are crucial for nutrient and electrolyte homoeostasis via specialised transport proteins.
Christopher‐Yannik Egler, Madlin Heidelberg, Marion Burmester, Karin Hustedt, Nadine Schnepel, Alexandra S. Muscher‐Banse   +5 more
wiley   +1 more source

Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells

BMC Research Notes, 2013
Background The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this
Wei Ruoxin, Stewart Elizabeth Anne, Amoaku Winfried M   +2 more
doaj   +1 more source

Expression Stability of Reference Genes in Larvae of Spodoptera frugipferda Under Azadirachtin Stress by Real-Time Quantitative PCR Analysis

Guangdong nongye kexue
【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU, Yuting HUANG, Xuanyue YU, Cuiting LIU, Xinyi XIE, Hao SHEN, Jintian LIN   +6 more
doaj   +1 more source

Evaluation of stable reference genes in white mustard (Sinapis alba) for qRT-PCR analysis under various stress conditions

The Indian Journal of Agricultural Sciences, 2020
White mustard (Sinapis alba L.) is a member of Brassicaceae family and is a source of various biotics stress resistance genes. S. alba is closely related to the cultivated Brassica juncea which exhibit susceptibility towards various pathogens ...
SHIKHA DIXIT, VINOD KUMAR JANGID, ANITA GROVER   +2 more
doaj   +1 more source