Results 1 to 10 of about 22,071,005 (304)

Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR [PDF]

open access: yesBiomarker Insights, 2012
1p/19q (1p and/or 19q) deletions are prognostic factors in oligodendroglial tumors (OT) and predict better survival after both chemotherapy and radiotherapy. While studying 1p/19q status as a potential variable within multivariate prognosis models for OT,
Abhishek Chaturbedi   +3 more
doaj   +3 more sources

Real-Time PCR Assay for Quantitative Mismatch Detection [PDF]

open access: yesBioTechniques, 2003
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively   +5 more
doaj   +3 more sources

Gene Dosage by Quantitative Real-Time PCR

open access: yesBioTechniques, 1999
J.-L. Boulay   +5 more
doaj   +4 more sources

Quantitative analysis of the dystrophin gene by real-time PCR [PDF]

open access: yesArchives of Biological Sciences, 2012
Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Maksimovic Nela   +12 more
doaj   +1 more source

Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose

open access: yesFermentation, 2022
Rummeliibacillus sp. TYF-LIM-RU47, isolated from the fermentation substrate of grain vinegar, could produce acetoin using a variety of carbon sources, including pentose, hexose and lignocellulose.
Guoyang Feng   +5 more
doaj   +1 more source

Real time quantitative PCR. [PDF]

open access: yesGenome Research, 1996
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies.
C A, Heid   +3 more
openaire   +2 more sources

Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

open access: yesMolecular Plant-Microbe Interactions, 2003
Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf   +9 more
doaj   +1 more source

Real-Time PCR for mRNA Quantitation

open access: yesBioTechniques, 2005
(Uploaded by Plazi for the Bat Literature Project) Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput.
Marisa L. Wong, Juan F. Medrano
openaire   +4 more sources

Differential gene expression in response to cold stress in Lepidium apetalum during seedling emergence

open access: yesBiologia Plantarum, 2012
Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived
H. X. Zhao, Q. Li, G. Li, Y. Du
doaj   +1 more source

Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR

open access: yesMicrobiology Spectrum, 2022
In countries where the incidence of typhoid fever is high, fecal material from short-term carriers of Salmonella Typhi contaminates inadequately treated water supplies.
Ellen E. Higginson   +9 more
doaj   +1 more source

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