Results 41 to 50 of about 22,071,005 (304)
Veterinary and Animal ScienceThe aim of this study was to establish a highly specific and sensitive method to detect Salmonella spp. based on conventional PCR and quantitative real-time PCR (qPCR) and to validate its application in clinical and animal samples.
Ningning Liu +5 more
doaj +1 more source
PCR primers for quantitative real-time PCR.
, 2013 PCR primers for quantitative real-time PCR.
Delphine M. Vauthay (298928) +9 more
core +1 more source
Quantitative real-time PCR primer sequence.
, 2022 Quantitative real-time PCR primer sequence.
Dong-Bao Chen (12481573) +2 more
core +1 more source
FEBS Letters, EarlyView.Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante +10 more
wiley +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Quantitative real-time PCR for the clinical detection of Helicobacter pylori
Genetics and Molecular Biology, 2007 Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human ...
Marcelo Lima Ribeiro +4 more
doaj +1 more source
Real-time quantitative RT-PCR.
, 2012 Real-time quantitative RT-PCR.
Hongjie Fan (167764) +4 more
core +1 more source
The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers
Molecular Oncology, EarlyView.The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce +11 more
wiley +1 more source
Making standards for quantitative real-time pneumococcal PCR [PDF]
Biomolecular Detection and Quantification, 2014 Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2).
Morpeth, Susan C. +3 more
openaire +2 more sources
Quantitative real-time PCR oligonucleotides.
, 2016 Quantitative real-time PCR oligonucleotides.
Raquel Fucho (85556) +10 more
core +1 more source