Results 41 to 50 of about 21,194,347 (300)

Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

open access: yesBioTechniques, 2022
For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian   +6 more
doaj   +1 more source

Quantitative Real-Time PCR

open access: yes, 2006
Changes in mRNA expression levels occur during physiological and pathological processes in the cardiovascular system. An increase inDNAtranscription results in increasedmRNAlevels and will subsequently result in increased protein levels that regulate processes inside and outside the cell.
Sluijter, J. P. G.   +2 more
openaire   +1 more source

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

open access: yesApplied Sciences
The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results.
Tianyu Zhu, Xin Liu, Xinqing Xiao
doaj   +1 more source

Development of a droplet digital PCR method for detection of porcine circovirus 4

open access: yesBMC Veterinary Research, 2023
Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu   +4 more
doaj   +1 more source

A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control [PDF]

open access: yes, 2005
We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of
Vázquez-Boland, JA   +9 more
core  

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR [PDF]

open access: yes, 2015
Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories.
Mitterbauer, G.   +316 more
core   +3 more sources

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

open access: yesFEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg   +14 more
wiley   +1 more source

Establishment of methods for the detection of Salmonella species by conventional and quantitative real-time PCR

open access: yesVeterinary and Animal Science
The aim of this study was to establish a highly specific and sensitive method to detect Salmonella spp. based on conventional PCR and quantitative real-time PCR (qPCR) and to validate its application in clinical and animal samples.
Ningning Liu   +5 more
doaj   +1 more source

A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection [PDF]

open access: yes, 2012
PMCID: PMC3393739This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
Bustin, SA   +14 more
core   +2 more sources

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