Results 1 to 10 of about 21,194,222 (201)

Establishment of methods for the detection of Salmonella species by conventional and quantitative real-time PCR [PDF]

Veterinary and Animal Science
The aim of this study was to establish a highly specific and sensitive method to detect Salmonella spp. based on conventional PCR and quantitative real-time PCR (qPCR) and to validate its application in clinical and animal samples.
Ningning Liu, Cong Liu, Lijun Chen, Guijuan Hao, Gang Zhu, Fangkun Wang   +5 more
doaj   +2 more sources

Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR [PDF]

Biomarker Insights, 2012
1p/19q (1p and/or 19q) deletions are prognostic factors in oligodendroglial tumors (OT) and predict better survival after both chemotherapy and radiotherapy. While studying 1p/19q status as a potential variable within multivariate prognosis models for OT,
Abhishek Chaturbedi, Liping Yu, Mark E Linskey, Yi-hong Zhou   +3 more
doaj   +3 more sources

Real-Time PCR Assay for Quantitative Mismatch Detection [PDF]

BioTechniques, 2003
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively, L. Chang, J.M. LeBon, Q. Liu, A.D. Riggs, J. Singer-Sam   +5 more
doaj   +3 more sources

Quantitative real time PCR for distinction between Pneumocystis jirovecii infection/colonization in hospitalized patients [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundIdentification of the opportunistic fungus Pneumocystis jirovecii in respiratory specimens presents challenges, particularly in differentiating between colonization and active infection.
Faezeh Rouhi, Mahzad Erami, Sepide Rastgufar, Maryam Jahani, Shima Aboutalebian, Shima Aboutalebian, Sajedeh Soltani, Hamed Fakhim, Hossein Mirhendi, Hossein Mirhendi   +9 more
doaj   +2 more sources

Making standards for quantitative real-time pneumococcal PCR [PDF]

Biomolecular Detection and Quantification, 2014
Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2).
John Anthony Gerard Scott, Jim Huggett, Susan Morpeth   +2 more
exaly   +3 more sources

Gene Dosage by Quantitative Real-Time PCR

BioTechniques, 1999
J.-L. Boulay, J. Reuter, R. Ritschard, L. Terracciano, R. Herrmann, C. Rochlitz   +5 more
doaj   +4 more sources

Quantitative analysis of the dystrophin gene by real-time PCR [PDF]

Archives of Biological Sciences, 2012
Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Maksimovic Nela, Anđelkovic Ana, Milic-Rasic Vedrana, Rakocevic-Stojanovic Vidosava, Kastratovic-Kotlica Biljana, Brankovic S., Damnjanovic Tatjana, Jekic Biljana, Bunjevacki Vera, Lukovic Ljiljana, Perovic Dijana, Cvjeticanin Suzana, Novakovic Ivana   +12 more
doaj   +1 more source

Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose

Fermentation, 2022
Rummeliibacillus sp. TYF-LIM-RU47, isolated from the fermentation substrate of grain vinegar, could produce acetoin using a variety of carbon sources, including pentose, hexose and lignocellulose.
Guoyang Feng, Xiaojun Fan, Yanna Liang, Chen Li, Junde Xing, Yongji He   +5 more
doaj   +1 more source

Real time quantitative PCR. [PDF]

Genome Research, 1996
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies.
C A, Heid, J, Stevens, K J, Livak, P M, Williams   +3 more
openaire   +2 more sources

Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

Molecular Plant-Microbe Interactions, 2003
Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf, Katja Manthey, Jasmin Doll, Andreas M. Perlick, Burkhard Linke, Thomas Bekel, Folker Meyer, Philipp Franken, Helge Küster, Franziska Krajinski   +9 more
doaj   +1 more source