Results 31 to 40 of about 1,295,509 (307)

The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas

Brazilian Journal of Medical and Biological Research, 2009
Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR)
A.C.M.F. Souza, D.R.V. Souza, S.S. Sanabani, R.R. Giorgi, I. Bendit   +4 more
doaj   +1 more source

MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines.

Clinical Chemistry
BACKGROUND In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines established standards for the design, execution, and reporting of quantitative PCR (qPCR) in research.
Stephen A. Bustin, J. M. Ruijter, M. V. D. van den Hoff, Mikael Kubista, M. Pfaffl, Gregory L. Shipley, Nham Tran, S. Rödiger, A. Untergasser, R. Mueller, T. Nolan, M. Milavec, M. Burns, Jim F. Huggett, J. Vandesompele, C. Wittwer   +15 more
semanticscholar   +1 more source

Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti

Scientific Reports, 2017
The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic expression studies utilizing quantitative real-time PCR (qPCR) have been conducted with ...
Najat Dzaki, Karima N. Ramli, Azali Azlan, I. Ishak, Ghows Azzam   +4 more
semanticscholar   +1 more source

Validation of internal control for gene expression study in soybean by quantitative real-time PCR

BMC Molecular Biology, 2008
BackgroundNormalizing to housekeeping gene (HKG) can make results from quantitative real-time PCR (qRT-PCR) more reliable. Recent studies have shown that no single HKG is universal for all experiments.
Bo Jian, Bin Liu, Yu-Rong Bi, Wen-Sheng Hou, Cun-Xiang Wu, T. Han   +5 more
semanticscholar   +1 more source

Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR

Scientific Reports, 2018
For reliable results, Reverse Transcription Quantitative real-time Polymerase Chain Reaction (RT-qPCR) analyses depend on stably expressed reference genes for data normalization purposes.
A. E. I. Gomes, Leonardo P Stuchi, Nathália Maria Gonçalves Siqueira, João Henrique, R. Vicentini, M. Ribeiro, Michelle Darrieux, L. Ferraz   +7 more
semanticscholar   +1 more source

Quantitative Real-Time PCR

, 2006
Changes in mRNA expression levels occur during physiological and pathological processes in the cardiovascular system. An increase inDNAtranscription results in increasedmRNAlevels and will subsequently result in increased protein levels that regulate processes inside and outside the cell.
Sluijter, J. P. G., Pasterkamp, G., de Kleijn, D. P. V.   +2 more
openaire   +1 more source

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

Applied Sciences
The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results.
Tianyu Zhu, Xin Liu, Xinqing Xiao
doaj   +1 more source

Development of a droplet digital PCR method for detection of porcine circovirus 4

BMC Veterinary Research, 2023
Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu, Xinru Zhang, Xueying Han, Jiaxing Liu, Lunguang Yao   +4 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

BioTechniques, 2022
For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian, Zhigang Chu, Linqing Wang, Huiyu Wang, Xiuyun Yuan, Si Wu, Yuzhen Yang   +6 more
doaj   +1 more source