Results 11 to 20 of about 1,295,509 (307)
Frontiers in Cellular and Infection Microbiology, 2022 Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming ...
Di Wang +7 more
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Biologia Plantarum, 2012 Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived
H. X. Zhao, Q. Li, G. Li, Y. Du
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Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR
Microbiology Spectrum, 2022 In countries where the incidence of typhoid fever is high, fecal material from short-term carriers of Salmonella Typhi contaminates inadequately treated water supplies.
Ellen E. Higginson +9 more
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Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2
Theranostics, 2020 In December 2019, a new coronavirus disease (COVID-19) outbreak occurred in Wuhan, China. Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the seventh coronavirus known to infect humans, is highly contagious and has rapidly expanded
Dandan Li, Jiawei Zhang, Jin-Ming Li
semanticscholar +1 more source
Quantitative real-time RT-PCR – a perspective [PDF]
Journal of Molecular Endocrinology, 2005 Abstract The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel ...
S A, Bustin +3 more
openaire +2 more sources
Biomolecular Detection and Quantification, 2017 Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids.
A. Forootan +5 more
semanticscholar +1 more source
Detection of Pork-Derived Ingredients Based on Real-time Quantitative PCR
Shipin gongye ke-ji, 2022 In order to accurately and reliably quantify pork-derived ingredients in meat products, single-copy gene (carcinoembryonic antigen-related cell adhesion molecule 2-like, CACA) in pig cell nucleus was screened by bioinformatics methods, with CACA gene as ...
Yao YANG +5 more
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Revista Brasileira de Zootecnia, 2020 To screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets ( Sus scrofa ) with intestinal damage using quantitative real-time PCR (qPCR) and ...
Xiaoyi Qi +5 more
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Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)
Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022 Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra +6 more
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Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
Plant Methods, 2019 A suitable reference gene is an important prerequisite for guarantying accurate and reliable results in quantitative real-time PCR (qRT-PCR) analyses. However, there is no absolute universality in reference genes among different species.
Canbin Chen +10 more
semanticscholar +1 more source