Results 41 to 50 of about 21,194,222 (201)

Real-time quantitative PCR primers.

open access: yes, 2020
Real-time quantitative PCR primers.
Samantha A. Brooks (8693760)   +4 more
core   +1 more source

Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

open access: yesBioTechniques, 2022
For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian   +6 more
doaj   +1 more source

Quantitative Real-Time PCR

open access: yes, 2006
Changes in mRNA expression levels occur during physiological and pathological processes in the cardiovascular system. An increase inDNAtranscription results in increasedmRNAlevels and will subsequently result in increased protein levels that regulate processes inside and outside the cell.
Sluijter, J. P. G.   +2 more
openaire   +1 more source

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

open access: yesApplied Sciences
The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results.
Tianyu Zhu, Xin Liu, Xinqing Xiao
doaj   +1 more source

Development of a droplet digital PCR method for detection of porcine circovirus 4

open access: yesBMC Veterinary Research, 2023
Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu   +4 more
doaj   +1 more source

A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control [PDF]

open access: yes, 2005
We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of
Vázquez-Boland, JA   +9 more
core  

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR [PDF]

open access: yes, 2015
Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories.
Mitterbauer, G.   +316 more
core   +3 more sources

A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection [PDF]

open access: yes, 2012
PMCID: PMC3393739This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
Bustin, SA   +14 more
core   +2 more sources

Development and field assessment of a quantitative PCR for the detection and enumeration of the noxious bloom-former Anabaena planktonica [PDF]

open access: yes, 2007
Anabaena planktonica is a harmful, bloom-forming freshwater cyanobacterium, which has arrived recently in New Zealand. In the short time since its incursion (
Rueckert, Andreas   +5 more
core   +2 more sources

Quantitative real-time PCR for the clinical detection of Helicobacter pylori

open access: yesGenetics and Molecular Biology, 2007
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human ...
Marcelo Lima Ribeiro   +4 more
doaj   +1 more source

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