Results 51 to 60 of about 21,194,222 (201)

Desenvolvimento de iniciadores e sondas para detecção de cry1A.105 e cry2Ab2 e aplicação de PCR e PCR em tempo real para detecção de OGM em alimentos [PDF]

, 2011
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Ciência dos Alimentos, Florianópolis, 2011Reação em Cadeia da Polimerase (PCR) e PCR quantitativa em tempo real são usadas para detecção ...
Dinon, Andréia Zilio
core  

Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. [PDF]

, 2011
One of the greatest challenges of implementing fast molecular detection methods as part of Legionella surveillance systems is to limit detection to live cells. In this work, a protocol for sample treatment with propidium monoazide (PMA) in combination
Soria-Soria, Elena, Lorena Martínez, Vicente Catalán, Raquel Múrtula, Múrtula, Raquel, Elena Soria-Soria, Nocker, Andreas, M. Adela Yáñez, Yáñez, M. Adela, Martínez, Lorena, Catalán, Vicente, Andreas Nocker   +11 more
core   +1 more source

Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon [PDF]

, 2008
PCR inhibition by nucleic acid extracts is a well known yet poorly described phenomenon. Inhibition assessment generally depends on the assumption that inhibitors affect all PCR reactions to the same extent; i.e.
Miller Robert F, Morris-Jones Stephen D, Tanya Novak, Garson Jeremy A, Jim F Huggett, Morris-Jones, SD, Zumla, A, Huggett, JF, Miller, RF, Green Clare, Zumla Alimuddin, Garson, JA, Novak Tanya, Novak, T, Jeremy A Garson, Clare Green, Stephen D Morris-Jones, Robert F Miller, Green, C, Huggett Jim F, Alimuddin Zumla   +20 more
core   +1 more source

Quantitative Determination of Lentiviral Vector Particle Numbers by Real-Time PCR

BioTechniques, 2001
Here, we describe a quantitative, DNAbased, real-time PCR approach to determine the number of lentivirus particles that are present in vector preparations.
M. Scherr, K. Battmer, U. Blömer, A. Ganser, M. Grez   +4 more
doaj   +1 more source

Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations

Journal of Veterinary Research, 2022
Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease.
Souza Camilla de, Coelho Mariana, Antonelo Daniel Silva, Passarelli Danielle, Rochetti Arina Lázaro, Fukumasu Heidge, Leite-Dellova Deise Carla Almeida   +6 more
doaj   +1 more source

Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control [PDF]

, 2005
Quantitation of circulating hepatitis B virus (HBV) DNA is important for monitoring disease progression and for assessing the response to antiviral therapy. Several commercial and 'in house' assays for HBV DNA quantitation have been described but many of
Tedder, RS, Ayliffe, U, Grant, PR, Ferns, RB, Garson, JA   +4 more
core  

Serum CCAT2 as a biomarker for adjuvant diagnosis and prognostic prediction of cervical cancer

Journal of Ovarian Research, 2022
Growing evidence indicates that lncRNA colon cancer-associated transcript 2 (CCAT2) is associated with cancers. However, the clinical value of CCAT2 in cervical cancer (CC) remains unclear.
Xiaoli Cao, Juan Yao, Meiqun Jia, Xianjuan Shen, Jinye Zhang, Shaoqing Ju   +5 more
doaj   +1 more source

Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages

BMC Genomics, 2022
Background Quantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require normalization with validated reference gene(s).
Jeongah Song, Jeonghee Cho, Jeongsik Park, Jeong Ho Hwang   +3 more
doaj   +1 more source

Development of real-time PCR for detection of Mycoplasma hominis [PDF]

, 2004
Background: Mycoplasma hominis is associated with pelvic inflammatory disease, bacterial vaginosis, post partum fever, sepsis and infections of the central nervous system often leading to serious conditions.
Christiansen, G, Fedder Jens, Birkelund, S., Christiansen Gunna, Gunna Christiansen, Fedder, J., Christiansen, G., Svenstrup, HF, Svenstrup Helle F, Baczynska Agata, Helle F Svenstrup, Baczynska, A, Fedder, J, Birkelund Svend, Baczynska, A., Svendstrup, H.F., Agata Baczynska, Jens Fedder, Svend Birkelund, Birkelund, S   +19 more
core   +1 more source

BactQuant: An enhanced broad-coverage bacterial quantitative real-time PCR assay

BMC Microbiology, 2012
Background Bacterial load quantification is a critical component of bacterial community analysis, but a culture-independent method capable of detecting and quantifying diverse bacteria is needed. Based on our analysis of a diverse collection of 16 S rRNA
Liu Cindy M, Aziz Maliha, Kachur Sergey, Hsueh Po-Ren, Huang Yu-Tsung, Keim Paul, Price Lance B   +6 more
doaj   +1 more source