Results 71 to 80 of about 21,194,222 (201)

Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae

Biotechnology & Biotechnological Equipment, 2017
Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods.
Parisa Azizi, Mohd Y. Rafii, Maziah Mahmood, Siti Nor Akmar Abdullah, Mohamed Musa Hanafi, Muhammad Abdul Latif, Mahbod Sahebi, Sadegh Ashkani   +7 more
doaj   +1 more source

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

Virology Journal, 2018
Background Human adenovirus (HAdV) serotypes 2, 3 and 7 are more prevalent than other serotypes and have been associated with severe pneumonia in pediatric children.
Fang-zhou Qiu, Xin-xin Shen, Meng-chuan Zhao, Li Zhao, Su-xia Duan, Chen Chen, Ju-Ju Qi, Gui-xia Li, Le Wang, Zhi-shan Feng, Xue-jun Ma   +10 more
doaj   +1 more source

PCR primers for quantitative real-time PCR.

, 2013
PCR primers for quantitative real-time PCR.
Delphine M. Vauthay (298928), Philippe Klee (298940), Audrey Guérardel (298931), Petra S. Hüppi (298943), Audrey Toulotte (298934), Paolo Meda (134110), Philippe Cettour-Rose (298937), Emmanuel Somm (298925), Michel L. Aubert (298942), Valérie M. Schwitzgebel (298944)   +9 more
core   +1 more source

自发性高血压大鼠血液miRNAs表达分析

Zhongguo shiyan zhenduanxue, 2014
目的本试验检测自发性高血压大鼠（SHR）血液中miR-448-5p、miR-128、miR-146a和miR-24-2*四种小分子RNA（miRNA）的表达,探讨高血压疾病与miRNA表达水平相关性,为人类高血压诊断及治疗提供新的靶标。方法应用荧光定量PCR方法对SHR大鼠和正常大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2*表达量进行检测。结果与正常对照组相比,SHR大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2 ...
鞠传静, 王旭渤, 梁斌斌, 李颖, 于冰   +4 more
doaj  

Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus

Frontiers in Veterinary Science
IntroductionThe African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques.
Silvia Dei Giudici, Piero Bonelli, Maria Giovanna Tilocca, Simona Cau, Pier Paolo Angioi, Anna Maria Sechi, Bruna Vodret, Annalisa Oggiano   +7 more
doaj   +1 more source

[Real time quantitative PCR].

Experimental & molecular medicine, 2002
So far, quantitative techniques, such as PCR and FISH, have been used to detect of DNA and RNA. However, it is difficult to measure and compare the exact amount of amplified products with the results of endpoint analysis in conventional PCR techniques.
openaire   +1 more source

Detection of Mycosphaerella graminicola in Wheat Leaves by a Microsatellite Dinucleotide Specific-Primer [PDF]

, 2011
Early detection of infection is very important for efficient management of Mycosphaerella graminicola leaf blotch. To monitor and quantify the occurrence of this fungus during the growing season, a diagnostic method based on real-time PCR was developed ...
Bahkali, A.H., Verreet, J.A., Abd-Elsalam, K., Moslem, M., Mohamed Moslem, Ali H. Bahkali, Wit, P.J.G.M., de, Pierre J. G. M. De Wit, Kamel Abd-Elsalam, Joseph-Alexander Verreet   +9 more
core   +1 more source

Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

Journal of Animal Science and Biotechnology, 2012
MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation.
Kang Kang, Peng Xiao, Luo Jun, Gou Deming   +3 more
doaj   +1 more source

HUMAN HERPESVIRUS 8 UPREGULATES ACTIVATING TRANSCRIPTION FACTOR 4 (ATF4) and REAL TIME PCR TO ASSESS TOTAL BACTERIAL LOAD IN CHRONIC WOUNDS. [PDF]

, 2010
I. Human Herpesvirus 8 Upregulates Activator Transcription Factor 4 BACKGROUND: Human herpesvirus 8 (HHV-8) is the primary etiologic agent of Kaposi’s sarcoma, a highly vascularised neoplasm of endothelial origin characterized by inflammation ...
Gentili, Valentina
core  

Quantitative real-time RT-PCR.

, 2013
Expression of (A) AR and PSA and (B) c-jun, KLK2, NKX3.1 and c-Myc mRNA levels in response to Doc or Pac was evaluated by quantitative real time PCR (qPCR).
Binshen Chen (481747), Martina Tinzl (481746), Julius Semenas (481749), Shao-Yong Chen (481748), Per-Anders Abrahamsson (481750), Nishtman Dizeyi (443546)   +5 more
core   +1 more source